Kit for determining homocysteine and preparation method thereof

The technology of a homocysteine ​​and a kit is applied in the field of a kit for the determination of homocysteine ​​and its preparation, which can solve the problems of complicated operation, poor accuracy and high cost, and achieve accurate detection results, simplified operation, The effect of short detection time

Inactive Publication Date: 2016-10-26
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a kit for measuring homocysteine ​​an...

Method used

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  • Kit for determining homocysteine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein:

[0073] Reagent R1:

[0074] Phosphate buffer 50mmol / L

[0075] Sodium chloride 80mmol / L

[0076] S-adenosylmethionine 0.8 mmol / L

[0077] Reduced coenzyme Ⅱ 5 KU / L

[0078] Tris(2-carboxyethyl)phosphine hydrogen chloride 1.8mmol / L

[0079] Sodium azide 0.7 g / L

[0080] The solvent is purified water

[0081] Reagent R2:

[0082] Phosphate buffer 50 mmol / L

[0083] HCY methyltransferase 2 KU / L

[0084] Glutamate dehydrogenase 2.5 KU / L

[0085] S-adenosyl homocysteine ​​hydrolase 3.0 KU / L

[0086] Bovine Serum Albumin 25 g / L

[0087] Sodium azide 0.7g / L

[0088] Its solvent is purified water.

Embodiment 2

[0090] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein:

[0091] Reagent R1:

[0092] Phosphate buffer 20mmol / L

[0093] Sodium chloride 120 mmol / L

[0094] S-adenosylmethionine 0.2mmol / L

[0095] Reduced coenzyme Ⅱ 2 KU / L

[0096] Tris(2-carboxyethyl)phosphine hydrogen chloride 0.6 mmol / L

[0097] Sodium azide 0.3g / L

[0098] The solvent is purified water

[0099] Reagent R2:

[0100] Phosphate buffer 80 mmol / L

[0101] HCY methyltransferase 1.0 KU / L

[0102] Glutamate dehydrogenase 1.5 KU / L

[0103] S-adenosyl homocysteine ​​hydrolase 4.0 KU / L

[0104] Bovine Serum Albumin 15 g / L

[0105] Sodium azide 1.1 g / L

[0106] Its solvent is purified water.

Embodiment 3

[0108] Kit preparation and method of use

[0109] 1. Prepare the reagent according to the content of the following components:

[0110] Reagent R1:

[0111] Phosphate buffer 50mmol / L

[0112] Sodium chloride 80mmol / L

[0113] S-adenosylmethionine 0.8 mmol / L

[0114] Reduced coenzyme Ⅱ 5 KU / L

[0115] Tris(2-carboxyethyl)phosphine hydrogen chloride 1.8mmol / L

[0116] Sodium azide 0.7 g / L

[0117] The solvent is purified water

[0118] Reagent R2:

[0119] Phosphate buffer 50 mmol / L

[0120] HCY methyltransferase 2 KU / L

[0121] Glutamate dehydrogenase 2.5 KU / L

[0122] S-adenosyl homocysteine ​​hydrolase 3.0 KU / L

[0123] Bovine Serum Albumin 25 g / L

[0124] Sodium azide 0.7g / L

[0125] The solvent is purified water;

[0126] 2. Parameter setting of automatic biochemical analyzer

[0127] (a) Detection temperature: 37°C;

[0128] (b) Detection wavelength: main wavelength 340nm, secondary wavelength 405nm;

[0129] (c) Reaction time: 8 minutes, among them, the i...

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Abstract

The invention discloses a kit for determining homocysteine and a preparation method thereof. The kit is composed of dual-liquid components of a reagent R1and a reagent R2 which are independent from each other. The reagent R1 is prepared from a phosphate buffer, sodium chloride, S-adenosylmethionine, a reduced coenzyme II, tri(2-carboxyl) phosphine hydrogen chloride and sodium azide; the reagent R2 is prepared from phosphate buffer, HCY transmethylase, glutamate dehydrogenase, S-adenosylhomocysteine hydrolase, bovine serum albumin and sodium azide. The preparation method comprises the steps that the reagents are prepared according to the component content; a to-be-tested sample is mixed with the reagent R1 and the reagent R2 to perform a sufficient reaction; a fully automatic biochemical analyzer is used for determining the reacted absorptivity difference; according to the absorptivity change value, the concentration of homocysteine in the sample is worked out. The kit has the advantages of being convenient to operate, high in accuracy and the like.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring homocysteine ​​and a preparation method thereof. Background technique [0002] Homocysteine, also known as homocysteine, is a sulfur-containing amino acid formed after demethylation of methionine. It is also the substrate for the synthesis of cystathionine by cystathionine β synthase. The total homocysteine ​​in the blood includes homocysteine, homocysteine ​​disulfide compound and cystine-homocysteine, three forms, most of them exist in protein binding, a small part in a free state. [0003] Plasma homocysteine ​​is an intermediate product of the methionine cycle, which is affected by genetic factors, nutritional status, liver and kidney functions, and metabolic status in some diseases. Many studies have shown that homocysteine ​​can be called the heart , an independent risk factor for brain and peripheral vascular disease, vitamin B6, f...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6815
Inventor 蔡晓辉庄庆华吴铮徐运
Owner ANHUI IPROCOM BIOTECH CO LTD
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