Method for selecting wild-cabbage-type rape varieties and materials by rape double haploid inducing line

A technology of Brassica napus and double haploids, which is applied in botany equipment and methods, plant genetic improvement, angiosperms/flowering plants, etc., can solve the problems of low induction efficiency, achieve excellent lodging resistance, increase yield, long time effect

Active Publication Date: 2016-11-09
CHENGDU ACAD OF AGRI & FORESTRY SCI
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  • Application Information

AI Technical Summary

Problems solved by technology

The earliest maize haploid induction line is stock6, which can only induce haploids in maize, and then the haploid plants undergo artificial chromosome doubling to form homozygous diploids (double haploids), and the induction efficiency Low, generally the induction efficiency is below 10% (calculated based on the number of haploids obtained from harvested seeds)

Method used

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  • Method for selecting wild-cabbage-type rape varieties and materials by rape double haploid inducing line
  • Method for selecting wild-cabbage-type rape varieties and materials by rape double haploid inducing line
  • Method for selecting wild-cabbage-type rape varieties and materials by rape double haploid inducing line

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Experimental program
Comparison scheme
Effect test

Embodiment １

[0082] see figure 1 , figure 2 , Figure 5 , Figure 7 , the Brassica oleracea double-low material "925100" was crossed with the recovery material "2150×Lijinte", and selected excellent single plants were self-crossed to F 9 Generations are still separated, F 10 Artificial emasculation during generation, and pollination with rapeseed double haploid induction line Y3380 obtained by the applicant, to obtain a large number of induced progeny seeds. f 11 The offspring (induced offspring) were planted and tested by flow cytometry, the breeding performance was normal, the ploidy (tetraploid) was normal, and there was no dominant trait of the induced line (dwarf) plants. The line was testcrossed with the polima cytoplasmic sterile line "Rong A0068", and the offspring of the testcross were all fertile and the traits of the individual plants within the line were consistent, indicating that the line was a restorer line. Therefore, a stable cytoplasmic male sterile restorer line ...

Embodiment 2

[0160] see figure 1 , figure 2 , Figure 5 , Figure 9 , the early-generation stable line P3-2 of Brassica napus was crossed with Zhongshuang 11, the hybrid offspring F1 was artificially emasculated, pollinated with the rapeseed double haploid induction line Y3380 obtained by the applicant, and the offspring were induced to self-bagging, F 2 The generation (induced offspring) was planted and flow cytometry was performed, the selection was normal, the ploidy (tetraploid) was normal, and there was no dominant trait (dwarf) of the induced line. Single bagging self-crossing of plants, F 3 Purity identification was carried out in several generations, among which the stable line 4653 was selected for test crossing with the polimaCMS cytoplasmic sterile line "Rong A0068", and the offspring of the test cross were all sterile, indicating that the induced stable line 4653 was a maintainer line, and the polimaCMS cytoplasmic maintainer line had a high oil content (more than 49%), lo...

Embodiment 3

[0162] see figure 1 , figure 2 , Figure 4 , Figure 10 , radish cytoplasmic ogura CMS type, whose restorer genes are not easy to obtain Brassica napus Chuanyou 36 is a radish cytoplasmic three-line hybrid rapeseed approved in the upper, middle and lower reaches of the Yangtze River in Sichuan Province, which contains radish cytoplasmic restorer genes. Chuanyou 36 was crossed with P3-2 to obtain aggregate hybrid F1, and F1 was emasculated with rapeseed double haploid induction line Y3560 obtained by the applicant for pollination. The F2 generation (induced offspring) was planted and subjected to flow cytometry detection, the selection was normal, the ploidy (tetraploid) was normal, and there was no dominant trait (dwarf) of the induced line. The plant was self-bagged, and the F3 generation was carried out. The purity was identified, and the stable strain 4707 was obtained. At the same time, the pollen of 4707 was tested with the stable radish cytoplasmic male sterile line...

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Abstract

A method for selecting wild-cabbage-type rape varieties and materials by a rape double haploid inducing line includes 1), determining target traits of a restorer line, a maintainer line and conventional variety breeding of wild-cabbage-type rapes; 2), performing crossing or convergent crossing on two or more wild-cabbage-type rapes with the target traits; 3), pollinating cross or backcross descendants by the rape double haploid inducing line; 4), verifying stability of the induced descendants; 5), performing test-crossing verification or yield and resistance verification on the stable descendants; 6), forming the stable restorer line and the stable maintainer line to enter crossbreeding combination or forming a conventional variety. The method has the advantages that rape materials or conventional varieties with breeding application values can be obtained rapidly and efficiently in a large scale, and the solid foundation is laid for wild-cabbage-type rape genetic breeding, breeding resource innovation, conventional rape variety breeding and breeding of new varieties of hybrid rapes; by the method, breeding speed and efficiency of hybrid or conventional varieties of the wild-cabbage-type rapes can be increased greatly, and manpower and material resources can be reduced.

Description

Technical field: [0001] The invention is related to agriculture, in particular to a method for breeding Brassica napus varieties and materials with rapeseed double haploid induction lines. Background technique: [0002] Rapeseed is the main oil crop in my country, Brassica napus ( Brassica napus , a compound species evolved from Brassica (aa, n=10) and cabbage (cc, n=9) through natural interspecific hybridization, which is tetraploid (2n=38 ), is the main type of cultivated rapeseed in the current rapeseed production. Breeding of new varieties of Brassica napus is firstly to select new inbred lines or genetically stable homozygous lines - homozygous lines (inbred lines), such as these homozygous lines in terms of resistance, yield, quality and other requirements It meets the production needs above all, and is finally recognized or approved as a new rapeseed variety (conventional variety) through the regional test. Secondly, test cross the homozygous line with the sterile ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/08
CPCA01H1/02A01H1/08A01H6/202A01H4/00
Inventor 付绍红杨进王继胜邹琼陶兰蓉康泽明李云唐蓉
Owner CHENGDU ACAD OF AGRI & FORESTRY SCI
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