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Rapid propagation method for tissue culture of loropetalum chinense

A technique of tissue culture and loropetalum, applied in the biological field, can solve the problems of low reproduction rate and achieve the effects of high survival rate of tissue culture, short growth cycle and stable genetic traits

Inactive Publication Date: 2016-11-09
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of sowing or cutting is mainly used for propagation, and the reproduction rate is generally low. Tissue culture can provide a new way for the propagation of loropetalum, but it has not been reported so far.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Select the seeds of loropetalum, rinse them with tap water for about 1 hour, then bathe them in 50% (v / v) sulfuric acid at 30°C for 10 minutes, rinse them with tap water for 5 minutes, grind them with a mortar for 5 minutes to remove the seed coat, rinse them with water, and use 75 for disinfection. % (v / v) alcohol solution for 20 seconds, then sterilized with 0.1% (w / v) mercuric solution for 8 minutes, rinsed with sterile water 5 times, each time for 3 minutes, inoculated in the modified GD+ZT2mg / L+NAA0.05mg / L+30g / L sucrose+7g / L agar medium for aseptic seedling culture, cut off the root of the induced aseptic seedlings and insert them into the medium to improve GD+6-BA2.0mg / L+NAA0.5mg / L +IBA0.15g / L+Ce(NO 3 ) 2 0.5mg / L+Na 2 MoO 4 0.5mg / L + sucrose 15mg / L + 7g / L agar medium for cluster bud proliferation culture, sterile seedlings and cluster bud induction culture conditions are natural light, temperature 25 ℃, humidity 65%, proliferation culture subculture 2 times T...

Embodiment 2

[0011] Select the seeds of loropetalum, rinse them with tap water for about 1 hour, then bathe them in 50% (v / v) sulfuric acid at 30°C for 10 minutes, rinse them with tap water for 5 minutes, grind them with a mortar for 5 minutes to remove the seed coat, rinse them with water, and use 75 for disinfection. % (v / v) alcohol solution for 20 seconds, then sterilized with 0.1% (w / v) mercuric solution for 8 minutes, rinsed with sterile water 5 times, each time for 3 minutes, inoculated in the modified GD+ZT2mg / L+NAA0.05mg / L+30g / L sucrose+7g / L agar medium for aseptic seedling culture, cut off the root of the induced aseptic seedlings and insert them into the medium to improve GD+6-BA2.0mg / L+NAA0.5mg / L +IBA0.15g / L+Ce(NO 3 ) 2 1.0mg / L+Na 2 MoO 4 1.0mg / L + sucrose 15mg / L + 7g / L agar medium for cluster bud proliferation culture, sterile seedlings and cluster bud induction culture conditions are natural light, temperature 25 ℃, humidity 65%, proliferation culture subculture 2 times T...

Embodiment 3

[0013] Select the seeds of loropetalum, rinse them with tap water for about 1 hour, then bathe them in 50% (v / v) sulfuric acid at 30°C for 10 minutes, rinse them with tap water for 5 minutes, grind them with a mortar for 5 minutes to remove the seed coat, rinse them with water, and use 75 for disinfection. % (v / v) alcohol solution for 20 seconds, then sterilized with 0.1% (w / v) mercuric solution for 8 minutes, rinsed with sterile water 5 times, each time for 3 minutes, inoculated in the modified GD+ZT2mg / L+NAA0.05mg / L+30g / L sucrose+7g / L agar medium for aseptic seedling culture, cut off the root of the induced aseptic seedlings and insert them into the medium to improve GD+6-BA2.0mg / L+NAA0.5mg / L +IBA0.15g / L+Ce(NO 3 ) 2 1.0mg / L+Na 2 MoO 4 0.5mg / L + sucrose 15mg / L + 7g / L agar medium for cluster bud proliferation culture, sterile seedlings and cluster bud induction culture conditions are natural light, temperature 25 ℃, humidity 65%, proliferation culture subculture 2 times T...

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Abstract

The invention researches a rapid propagation method for tissue culture of loropetalum chinense. The rapid propagation method comprises the following steps: sterilizing a seed, culturing a sterile seedling, inducing an axillary bud, propagating a cluster bud, inducing rooting, strengthening the seedling, transplanting, and the like. According to the rapid propagation method, loropetalum chinense is taken as a raw material, the influence of different hormone combinations to rapid propagation of loropetalum chinense is compared and analyzed, and thus a certain basis is made for establishment of a rapid propagation system, blastogenesis improvement and the like of the plant.

Description

technical field [0001] The invention relates to the culture of loropetalum tissue culture under the condition of tissue culture, and belongs to the field of biotechnology. Background technique [0002] Loropetalum, Loropetalum chinensis (R. Br.) Oliv., Hamamelidaceae, also known as P. chinensis, shrub, rarely a small tree, up to 12 meters high, 30 cm in diameter; branchlets have rust-colored stellate hairs, 4 species and 1 variety, distributed In the subtropical region of eastern Asia, there are 3 species and 1 variant in my country, which are produced in the middle and lower reaches of the Yangtze River and the areas south of and north of the Tropic of Cancer. It is a shade-loving plant, but it does not reject sunlight. It is often used as green seedlings, such as fences and green belts, and it is mostly born in mountains and hills. Flowers contain quercetin and isoquercitrin; leaves contain gallic acid, tannins, flavonoids (mainly quercetin). The roots, leaves, flowers ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 杨成东
Owner NANJING ZELANG AGRI DEV
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