Method for extracting tea saponin from camellia oleosa seed meal

A technology of camellia oleifera seed meal and tea saponin, which is applied in the field of tea saponin extraction, can solve the problems of inability to achieve high purity, limit the application range of tea saponin, and low yield and purity of tea saponin, so as to improve the utilization value, Broad market application prospects and the effect of increasing income sources

Inactive Publication Date: 2016-11-09
福建三九军大生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem that the present invention mainly solves: for the traditional tea saponin that occurs in the extraction process, the yield and purity of tea saponin are low during water immersion extraction, which limits the application range of tea saponin, and during organic solvent extraction, The methanol used is poisonous. Although the purity of ethanol extraction is improved compared with the water extraction method, the problem of high purity cannot be achieved. A method for extracting tea saponin from camellia oleifera seed dregs is provided. The present invention uses camellia oleifera seed dregs as raw material. After pulverization, leaching and defatting with ethyl acetate, and then treating with enzymes to fully release the active ingredients in the cells, then using ethanol and ultrasonic extraction, and then using urea and dry ice to cool down and recrystallize to precipitate tea saponin. Heat the precipitate to volatilize the residual ethanol, decompose the urea, and finally obtain high-purity tea saponin solid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0018] Weigh 200g of Camellia oleifera seed meal, put it on a stone mill and grind it into powder, collect 80 mesh Camellia oleifera seed meal powder, transfer it to a Soxhlet extractor filled with 1L ethyl acetate, and extract at 75°C After 1 hour, remove the filtrate by suction filtration, place the obtained filter cake in an oven at 60°C, and dry it for 2 hours to obtain defatted camellia oleifera seed meal powder; weigh 30 g of the above obtained defatted camellia oleifera seed meal powder and add it to a beaker filled with 100 mL of deionized water , then add 2g of cellulase and 2g of pectinase, then place the beaker on a digital speed measuring constant temperature magnetic stirrer, heat up to 45°C, adjust the speed to 300r / min, stir for 30min, then heat up to 80°C , add 200mL of ethanol solution with a mass concentration of 70%, then transfer the beaker to an ultrasonic oscillator, adjust the ultrasonic frequency to 25kHz, oscillate for 10min, transfer the mixed solution...

example 2

[0021]Weigh 260g of Camellia oleifera seed meal, put it on a stone mill and grind it into powder, collect 90 mesh Camellia oleifera seed meal powder, transfer it to a Soxhlet extractor filled with 2L ethyl acetate, and extract at 77°C After 1.5 hours, remove the filtrate by suction filtration, place the obtained filter cake in an oven at 63°C, and dry it for 3 hours to obtain the degreased camellia oleifera seed meal powder; weigh 40 g of the degreased camellia oleifera seed meal powder obtained above, and add it to a beaker filled with 180 mL of deionized water Add 3g of cellulase and 3g of pectinase, and then place the beaker on a digital speed measuring constant temperature magnetic stirrer, heat up to 48°C, adjust the speed to 360r / min, stir for 40min, then heat up to 82 ℃, add 260mL ethanol solution with a mass concentration of 75%, then transfer the beaker to an ultrasonic oscillator, adjust the ultrasonic frequency to 30kHz, and shake for 15 minutes, then transfer the mi...

example 3

[0024] Weigh 300g of Camellia oleifera seed meal, put it on a stone mill and grind it into powder, collect 100 mesh Camellia oleifera seed meal powder, transfer it to a Soxhlet extractor filled with 1-3L of ethyl acetate, and place it at 80°C , extract for 2 hours, remove the filtrate by suction filtration, place the obtained filter cake in an oven at 65°C, and dry it for 4 hours to obtain defatted camellia oleifera seed meal powder; weigh 50 g of the above obtained defatted camellia oleifera seed meal powder, add 200 mL of deionized water Add 4g of cellulase and 4g of pectinase to the beaker, then place the beaker on a digital display speed measuring constant temperature magnetic stirrer, heat up to 50°C, adjust the speed to 400r / min, stir and react for 50min, then heat up to 85°C, add 300mL ethanol solution with a mass concentration of 80%, then transfer the beaker to an ultrasonic oscillator, adjust the ultrasonic frequency to 35kHz, shake for 20min, transfer the mixed solut...

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Abstract

The invention discloses a method for extracting tea saponin from camellia oleosa seed meal, and belongs to the field of tea saponin extraction. The method comprises the steps that the camellia oleosa seed meal is taken as raw materials to be pulverized and then sequentially subjected to ethyl acetate leaching degreasing and enzyme treating, so that effective ingredients in cells of the camellia oleosa seed meal are fully released; ultrasonic extraction is conducted through cooperation of ethyl alcohol, and urea is utilized to cooperate with dry ice for cooling and recrystallizing, so that tea saponin precipitates; the precipitates are heated, so that residual ethyl alcohol is volatilized and urea is decomposed, and the high-purity tea saponin solid is finally obtained. According to the method, the obtained tea saponin is free of organic solvent residues, the urea can be completely decomposed to be taken away by nitrogen flow after being heated, and the purity of the obtained tea saponin can reach 98% or above.

Description

technical field [0001] The invention discloses a method for extracting tea saponin from camellia oleifera seed dregs, belonging to the field of tea saponin extraction. Background technique [0002] Camellia oleifera seeds of camellia oil tree can be pressed to obtain high-quality edible oil. The oleic acid content of camellia oleifera seeds is as high as 86%, and the linoleic acid content is 7-10%, which can be compared with olive oil. Camellia oleifera is mostly distributed in the southern region. China produces 1 million tons of camellia oleifera seeds per year, and the output of the remaining camellia oleifera seed meal is about 690,000 tons per year, and it shows an increasing trend every year. [0003] In ancient times, tea oil was obtained by pressing tea seeds at high temperature, and tea seed meal can be used as shampoo, mosquito coils, soap, etc. Tea seed meal is rich in nutrients, such as residual oil, protein, tea saponin, crude fiber and sugar, and can be widely...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08
CPCC07H1/08C07H15/256C07J63/008
Inventor 周荣许博伟
Owner 福建三九军大生物科技有限公司
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