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Polypeptide monomer molecule based on intracellular enzyme-catalyzed polymerization and its application in the construction of nanomaterials

A technology for catalyzing polymerization and intracellular enzymes, which is applied in the field of polypeptide monomer molecules and their application in the construction of nanomaterials, and can solve the problems of low assembly efficiency and poor controllability.

Active Publication Date: 2019-06-25
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the above achievements have been made, the supramolecular assembly of peptides in vivo still faces problems such as poor controllability and low assembly efficiency in complex environments.

Method used

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  • Polypeptide monomer molecule based on intracellular enzyme-catalyzed polymerization and its application in the construction of nanomaterials
  • Polypeptide monomer molecule based on intracellular enzyme-catalyzed polymerization and its application in the construction of nanomaterials
  • Polypeptide monomer molecule based on intracellular enzyme-catalyzed polymerization and its application in the construction of nanomaterials

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Experimental program
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Effect test

Embodiment 1

[0085] Polypeptide monomer molecule based on intracellular enzyme-catalyzed polymerization, its structure is designed as follows: functional molecule, glutamine end of polymerization active site, elastin-like repeat polypeptide and lysine end of polymerization active site are connected sequentially.

[0086] Among them, the functional molecule is a DBD fluorescent signal molecule; the glutamine terminal sequence of the polymerization active site is the sequence RQL shown in SEQ ID NO.2; the elastin-like repeat polypeptide sequence is the sequence shown in SEQ ID NO.25: GVGFP; The lysine end of the polymerization active site is K (Lys), and the structure of the polypeptide monomer molecule is shown below.

[0087]

Embodiment 2

[0089] Polypeptide monomer molecule based on intracellular enzyme-catalyzed polymerization, its structure is designed as follows: functional molecule, glutamine end of polymerization active site, elastin-like repeat polypeptide and lysine end of polymerization active site are connected sequentially.

[0090] Among them, the functional molecule is a DBD fluorescent signal molecule; the glutamine terminal sequence of the polymerization active site is the sequence LQR shown in SEQ ID NO.1; the elastin-like repeat polypeptide sequence is the sequence shown in SEQ ID NO.24: VPHVG; The lysine end of the polymerization active site is K(Lys).

Embodiment 3

[0092] Polypeptide monomer molecule based on intracellular enzyme-catalyzed polymerization, its structure is designed as follows: functional molecule, glutamine end of polymerization active site, elastin-like repeat polypeptide and lysine end of polymerization active site are connected sequentially.

[0093] Among them, the functional molecule is a DBD fluorescent signal molecule; the glutamine terminal sequence of the polymerization active site is the sequence RQL shown in SEQ ID NO.2; the elastin-like repeat polypeptide sequence is the sequence shown in SEQ ID NO.3: GVGVP; The lysine end of the polymerization active site is K(Lys).

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Abstract

The invention relates to a polypeptide monomer molecule based on intracellular enzyme catalyzed polymerization and application thereof in construction of nano materials especially elastin-like nano materials. The polypeptide monomer molecule is composed of a functional molecule, a polymerization active site and elastin-like repeat polypeptide, and can efficiently polymerize under the catalysis of transglutaminase, the formed polymer has temperature sensitive characteristic, and by changing the polypeptide sequence of monomer molecules, and the three-dimensional structure, conversion temperature, pore size and other parameters of the polymer can be adjusted. The polymer can achieve in situ controllable synthesis in living cells, and form a nano structure. The preparation method of the nano material can realize real-time in-situ imaging or high retention rate drug delivery in living cells, and has good application prospects.

Description

technical field [0001] The invention relates to the technical field of nanomaterial production, in particular to a polypeptide monomer molecule and its application in the construction of nanomaterials, in particular to a polypeptide monomer molecule based on intracellular enzyme-catalyzed polymerization and its application in the construction of elastin-like nanomaterials. application in materials. Background technique [0002] Controllable preparation of nanomaterials is a consistent pursuit in the field of materials. This controllability is not only reflected in the uniformity of the nanoscale and the uniformity of surface chemical properties, but also in the controllability of the preparation method. In recent years, for the application in the biological field, the preparation of nanomaterials in vitro and their biological functions have been widely developed, but the biological safety, the stability of materials in complex physiological environments, and the metabolism o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/06C07K7/08C07K1/107A61K47/42
CPCA61K47/42C07K7/06C07K7/08
Inventor 王浩李莉莉乔圣林刘伟娇马洋
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA