System for preparing biomass monomer through microbial coculture

A co-cultivation and biomass technology, applied in the field of biomass monomer preparation system, can solve the problems of high energy consumption, low fermentation efficiency, continuous fermentation, etc., and achieve the effect of improving efficiency and reducing energy consumption

Pending Publication Date: 2016-11-09
吉林中之林农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing microbial fermentation to prepare biomass monomers still has problems such as the need for biomass sterilization, huge energy consumption, fermentation batches, continuous fermentation, obvious antagonism, and low fermentation efficiency.

Method used

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  • System for preparing biomass monomer through microbial coculture

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Experimental program
Comparison scheme
Effect test

Embodiment １

[0021] Select goats as ruminant animals, select the biomass to be used as the carbon source after straw separation and treatment, feed the experimental animals, gradually reduce the addition of concentrated feed, and feed the prepared co-cultivation of ethanol-producing microorganisms, which can use five-carbon sugar and Six-carbon sugar Candida, fed to experimental animals for one month. Absorb the rumen content by using negative pressure gastric tube method, anaerobically roll the tube, purify goat rumen microorganisms, and cultivate them in an anaerobic incubator. After culturing for a period of time, gradually add ethanol-producing microbial metabolites to the medium for co-cultivation direction acclimatization Cultivation, sent to a small continuous culture and membrane separation device for rumen microbial amplification culture, the purified rumen microbial fiber decomposing fungi include Caecomyes single-center thallus, single-flagellate zoospores, spherical, branched rh...

Embodiment 2

[0023]Select ruminant dairy cows, select the biomass to be used as the carbon source after straw separation and treatment, feed the experimental animals, gradually reduce the addition of concentrated feed, and add at the same time to prepare ethanol-producing microorganisms for co-cultivation, which can use five carbons Candida of sugars and six-carbon sugars, fed to experimental animals for more than one month. Absorb the rumen content by negative pressure gastric tube method, anaerobically roll the tube, purify the rumen microorganisms of cows, and cultivate them in an anaerobic incubator. After a period of cultivation, gradually add ethanol-producing microbial metabolites to the medium for co-cultivation direction acclimatization Cultivated, sent to a small continuous culture and membrane separation device for enlarged culture of rumen microorganisms, and purified rumen microorganisms Ruminococcus. Rumen microorganisms are put into medium-sized anaerobic continuous culture ...

Embodiment 3

[0025] Select ruminant yellow cattle, select the biomass to be used as the carbon source after straw separation and treatment, feed the experimental animals, gradually reduce the addition of concentrated feed, and feed the prepared co-culture ethanol-producing microorganisms, which can use five-carbon sugar and Six-carbon sugar ethanol-producing bacteria, fed to experimental animals for more than one month. Absorb the rumen content by negative pressure gastric tube method, anaerobically roll the tube, purify cattle rumen microorganisms, and cultivate them in an anaerobic incubator. After culturing for a period of time, gradually add ethanol-producing microbial metabolites to the medium for co-cultivation direction acclimatization Culture, sent to small continuous culture and membrane separation device for rumen microorganism amplification culture, purified rumen microorganism Neocallimastix single-center bacteria, multi-flagellated zoospores, rich silky rhizoids N.frontalis, N....

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Abstract

The invention discloses a system for preparing a biomass monomer through microbial coculture. With biomass as a substrate, through process regulation and control over special equipment, coculture of biomass degrading microbes and target monomer generating microbes is achieved to prepare a target monomer. The system is characterized in that by coculturing rumen microbes and target product microbes and by regulating and controlling the equipment capable of separating a product in situ, commensalism of the rumen microbes and the target product microbes and high-efficiency preparation of a target product are achieved.

Description

technical field [0001] The invention relates to a system for preparing biomass monomers, in particular to a system for preparing biomass monomers through co-cultivation of microorganisms. Background technique [0002] In recent years, a variety of methods and systems for preparing biomass monomers using biological methods using non-food biomass as raw materials have emerged. Some choose enzyme preparations to saccharify biomass and then combine fermentation with yeast, but the cost of enzyme preparations is too high. Some choose the integrated fermentation of aerobic microorganisms and yeasts to prepare biomass ethanol, because the yield of aerobic fermentation is low. The existing microbial fermentation to prepare biomass monomers still has problems such as the need for biomass sterilization, huge energy consumption, fermentation batches, continuous fermentation, obvious antagonism, and low fermentation efficiency. Contents of the invention [0003] The purpose of the i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12N1/14C12N1/16C12M1/00C12R1/01C12R1/145C12R1/645C12R1/84
CPCC12M23/58C12M43/00C12N1/14C12N1/16C12P7/10Y02E50/10
Inventor 侯哲生宋德武马云海郑勇王文刚佟金石敏刘鹏刘海亮于丛鸣薛振军韩国顺连建伟王九刚刘发现梁嗣超张金翠刘跃宋家升王德林陈胜任露泉殷敬华
Owner 吉林中之林农业科技有限公司
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