Immunocolloidal gold test strip capable of detecting aspergillus and cryptococcus simultaneously

Abstract

The invention discloses an immunocolloidal gold test strip capable of detecting aspergillus and cryptococcus simultaneously. The immunocolloidal gold test strip comprises a test strip cassette and a matched test strip body, wherein the test strip cassette is provided with a sample cell and an observation window. The test strip body is formed by a rigid polyvinyl chloride lining plate, a nitrocellulose membrane, a left colloidal gold conjugate pad, a middle colloidal gold conjugate pad and a water absorption pad in a sequential lap joint mode, wherein the left colloidal gold conjugate pad is coated with colloidal-gold-labeled galactomannan resisting monoclonal antibodies and colloidal-gold-labeled bovine serum albumin-dinitrobenzene, the middle colloidal gold conjugate pad is coated with colloidal-gold-labeled cryptococcus capsular polysaccharide resisting monoclonal antibodies and colloidal-gold-labeled bovine serum albumin-dinitrobenzene, the nitrocellulose membrane is provided with a left detection line coated with galactomannan resisting monoclonal antibodies, a middle detection line coated with cryptococcus capsular polysaccharide resisting monoclonal antibodies, and a quality control line coated with dinitrobenzene resisting antibodies in sequence in parallel. The test strip is capable of detecting aspergillus and cryptococcus simultaneously, easy and convenient to operate, rapid and accurate in detection and high in sensitivity and specificity.

Description

technical field [0001] The invention belongs to the technical field of clinical medical diagnosis and in vitro fungus detection, in particular to an immune colloidal gold test paper for simultaneously detecting aspergillus and cryptococcus. Background technique [0002] In recent years, with the extensive abuse of corticosteroids, immunosuppressants, and various antibiotics, as well as the development of medical technologies such as organ transplantation and invasive examinations, the incidence of invasive fungal infections has increased. In particular, common clinical fungi such as Aspergillus and Cryptococcus have extremely high mortality rates. At present, the clinical treatment of fungal infections is relatively simple and effective drugs are lacking, so early, rapid and convenient in vitro fungal detection and diagnosis are very important. At present, the commonly used clinical fungal detection methods can be divided into culture method and non-culture method, specific...

AI Technical Summary

Problems solved by technology

At present, there is no mature immunocolloidal gold test paper detection technology for Aspergillus or cryptococcus in China, and all tests require pretreatment of blood samples to obtain serum for detection, and it is impossible to detect multiple fungal infections at the same time, which limits the actual fungal infection Detection efficiency and clinical promotion

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