Micro-fluidic chip and preparation method of alginate magnetic microspheres

Abstract

The invention discloses a micro-fluidic chip and a preparation method of alginate magnetic microspheres. Silicon oil, a sodium alginate solution, a magnetic bead solution and a calcium chloride solution are separately injected to an oil phase inlet, a sodium alginate liquid phase inlet, a magnetic bead liquid phase inlet and a calcium chloride liquid phase inlet by virtue of an injection pump at the same time; under the extrusion-shear effect of the silicon oil, a laminar flow is formed by the sodium alginate solution, the magnetic bead solution and the calcium chloride solution in the part of a droplet generating structure, and finally, three-phase droplets, which consist of three substances, namely sodium alginate, magnetic beads and calcium chloride, and are not completely mixed, are formed in the part of the droplet generating structure; the three-phase droplets are fully mixed in a snake-shaped passage, so that the magnetic microspheres, which are uniform in morphology, are formed; and finally, the magnetic microspheres flow out of the micro-fluidic chip. The shortcomings of a conventional microsphere preparation method which is strict in requirements on preparation conditions, complex in equipment and the like are overcome; and mild internal environment of the microspheres and the physicochemical characteristics of the magnetic beads are guaranteed.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and relates to a method for preparing a microfluidic chip and alginate magnetic microspheres. Background technique [0002] Microfluidic chip technology originated in the 1990s. Fluids are controlled by networked microchannels, and the functions of the entire laboratory, including sample pretreatment, reaction, separation, and detection, are integrated on the microchip, which greatly improves the analysis speed. improve. [0003] Microsphere technology can improve the physical properties (color, appearance, apparent density, etc.) of encapsulated substances by encapsulating substances; improve the stability and durability of substances. Alginate is a natural polysaccharide compound derived from brown algae. It has the advantages of wide sources, safety and non-toxicity. Alginate has been more and more used in drug sustained and controlled release, especially micro / nano sustained and c...

AI Technical Summary

Problems solved by technology

Among them, coaxial electrostatic spraying requires a high voltage of nearly 10KV, complex equipment, and high temperature requirements. If it is lower than 0°C or higher than 50°C, the preparation of magnetic microspheres will fail; the co-precipitation method is simple to synthesize, but the shape is poor. , low purity; the sol-gel method has complex synthesis process, poor sinterability, and narrow application range; hydrothermal method, high temperature requirements, long cycle; microemulsion method, the synthetic magnetic material is a core-shell mixed type

[0005] In the preparation process of the above method, organic solvents or relatively large amounts of surfactants are often added, which has potential toxicity hazards in the medical field, food industry, water pollution control and other fields.

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