A method for preparing Lycium barbarum polysaccharides with different molecular weights by ultrasonic coupling membrane separation and fractionation

Abstract

The invention relates to a method for preparing lycium barbarum polysaccharides different in molecular weight through ultrasonic coupling membrane separation and classification. The method includes: utilizing ultrasonic waves to extract lycium barbarum residue to obtain polysaccharide liquid, and standing overnight; using a nylon net for solid-liquid separation, and centrifuging; using a membrane of 67000 in molecular weight for ultrafiltration; using a membrane of 100000 in molecular weight for ultrafiltration, and respectively collecting ultrafiltrates with molecular weight less than 67000, from 67000 to 100000 and more than 100000 to obtain three lycium barbarum polysaccharide solutions different in molecular weight; concentrating and lyophilizing to obtain lycium barbarum polysaccharide powder different in application. The method has the advantages of being energy-saving, time-saving, pollution-free and environment-friendly and can be used for industrial large-scale production; the lycium barbarum polysaccharides which are classified after membrane separation and different in molecular weight are suitable for different food industries, application range is expanded, and effect is obvious.

Description

technical field [0001] The invention relates to a preparation method of Lycium barbarum polysaccharide, in particular to a method for preparing Lycium barbarum polysaccharide with different molecular weights by ultrasonic coupling membrane separation and fractionation. [0002] technical background [0003] Lycium bararum (Lycium bararumL ) is a traditional Chinese herbal medicine with the same origin as medicine and food in my country. It has the functions of nourishing yin and kidney, nourishing liver and improving eyesight. In recent years, the artificial cultivation of wolfberry in the Qaidam Basin of Qinghai has developed rapidly. By 2015, the total planting area reached 300,000 mu, surpassing that of Ningxia. The unique natural conditions and clean ecological environment of the Qaidam Basin, without any pollution within a radius of 100 kilometers, large temperature difference between day and night, light diseases and insect pests, less frequency of pesticide application...

AI Technical Summary

Problems solved by technology

The ethanol precipitation method takes advantage of the fact that most sugars are insoluble in organic solvents such as ethanol and acetone. By adding organic solvents, the polysaccharides insoluble in solvents are precipitated, so as to achieve and soluble small molecules (such as small pigment molecules, salts, etc.) small molecules, etc.) to obtain crude polysaccharides; column chromatography has high separation efficiency, but gel columns are expensive, and are usually used in laboratories to prepare a small amount of pure products, which are not suitable for large-scale industrial use; Sevage The protein removal method has the disadvantages of large consumption of organic reagents, complicated operation, and serious loss of polysaccharides.

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