Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for achieving overall genetic transformation of wheat

A genetic transformation and overall technology, applied in the field of genetic engineering, can solve the problems of low genetic transformation efficiency, long transformation cycle, and cumbersome operation process of wheat, and achieve the effects of reducing identification workload, improving transformation efficiency, and simplifying the experimental process

Inactive Publication Date: 2016-11-23
JILIN NORMAL UNIV
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above deficiencies, the purpose of the present invention is to overcome the difficulties of low genetic transformation efficiency of existing wheat, long transformation period and cumbersome operation process, and provide a method for overall infection of wheat seeds to improve the transformation efficiency of wheat

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for achieving overall genetic transformation of wheat
  • Method for achieving overall genetic transformation of wheat
  • Method for achieving overall genetic transformation of wheat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] Below in conjunction with implementation case the present invention will be further described:

[0036] 1. Preparation and sterilization of culture medium

[0037] 1. Preparation of LB liquid medium: Weigh 10 g NaCl, 10 g peptone and 5 g yeast extract into a 1 L Erlenmeyer flask, add distilled water to 1 L, dissolve, adjust the pH value to 7.0, Seal with parafilm, then use 121 o C autoclave for 20 minutes, cool and place at 4 o C in the refrigerator, spare;

[0038] 2. Preparation of LB solid medium: Weigh 10 g NaCl, 10 g peptone, 5 g yeast extract and 15 g agar into a 1 L Erlenmeyer flask, add distilled water to 1 L, dissolve, and adjust the pH Value to 7.0, seal with parafilm, then use 121 o C autoclave with moist heat for 20 minutes, pour into a sterile petri dish, cool and place at 4 o C in the refrigerator, spare;

[0039] 3. Preparation of sterile water: Add distilled water into a 1 L Erlenmeyer flask, seal it with a parafilm, and then use 121 o C autoclave...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for achieving overall genetic transformation of wheat in the technical field of genetic engineering, and particularly relates to a method for achieving overall impregnation of wheat seeds. The method comprises the steps of measuring a physiological imbibition curve of mature wheat seeds, culturing agrobacterium tumefaciens containing target genes, sterilizing the wheat seeds, transferring the sterilized wheat seeds into an agrobacterium tumefaciens solution for impregnation, conducting co-culturing, screening resistant plants, conducting rooting culture on the resistant plants and conducting seedling exercising on transformation plants and transplanting. According to the wheat genetic transformation method mediated by the agrobacterium tumefaciens, the transformation efficiency is greatly improved, the number of obtained transformation plants is greatly increased, the experimental period is shortened by two months to three months, the experimental process and identification workload after transformation are simplified, and important significance in theoretical research of wheat genetic engineering and genetic breeding is achieved.

Description

technical field [0001] The invention relates to a method for genetic transformation of wheat, which belongs to the technical field of genetic engineering, in particular to a method for overall infection and transformation of wheat seeds. Background technique [0002] wheat( Triticun aestivum L. ) is one of the most important food crops in the world, and is also an important source of plant protein for humans. The protein content of wheat accounts for about 39% of grain protein. The planting area and production of wheat account for about 30% of the cereal planting area. Wheat flour contains about 70%-80% starch, 10%-15% protein, 1%-2% lipid and other components. The protein content and quality of wheat play an important role in flour processing and amino acid nutritional quality . The total protein content of wheat in my country is not lower than that in foreign countries, but there is a big difference in processing quality, as long as it is because of the low content of p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/84A01H4/00A01H5/00
Inventor 王晶周晓馥未晓巍
Owner JILIN NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com