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Limulus plasma proteolytic peptide with antioxidant activity and its preparation method and application

An antioxidant activity and plasma protein technology, which is applied in the field of Limulus plasma proteolytic peptide and its preparation, can solve problems such as waste and discard of Limulus plasma, and achieve the effects of improving life quality, simple preparation method and easy mass production.

Active Publication Date: 2020-02-14
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on the utilization of horseshoe crab blood in my country is mainly to extract the amebocytes in the horseshoe crab blood, and then isolate the small molecular active peptide with antibacterial and anti-endotoxin activity from the amebocytes and produce the limulus reagent. Peptides are used as lead compounds to develop new antibacterial, antiviral, and antitumor drugs. However, the remaining Limulus plasma after the separation of amebocytes from Limulus blood exceeds the human consumption standard, and the Limulus plasma is generally discarded as waste, which has caused great harm. waste
Up to now, no direct research on Limulus plasma proteolytic peptides has been reported in China. With people's demand for peptide foods and medicines, especially some patients and special groups, such as patients with digestive tract diseases, hypoallergenic patients, and the elderly , athletes, etc., it is of great significance to study Limulus blood hydrolyzed peptide and its preparation method

Method used

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  • Limulus plasma proteolytic peptide with antioxidant activity and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0026] (1) heat-treat Limulus blood in a 90°C water bath for 3 hours, filter it with suction, and wash the denatured protein with distilled water;

[0027] (2) Use trypsin to enzymatically hydrolyze the denatured protein in step (1) to obtain hydrolyzed peptides, centrifuge, and take the supernatant for later use;

[0028] (3) The supernatant was passed through a SephadexG-50 Sephadex column, and the component with the largest absorbance at a wavelength of 230nm was collected to obtain the hydrolyzed peptide of limulus plasma protein, which was freeze-dried to obtain the hydrolyzed peptide powder.

Embodiment 2

[0030] (1) Heat the horseshoe crab blood in a water bath at 100°C for 3 hours, filter it with suction, and wash the denatured protein with distilled water;

[0031] (2) Use trypsin to hydrolyze the denatured protein in step (1) to obtain hydrolyzed peptides, centrifuge, and take the supernatant for later use;

[0032] (3) The supernatant was passed through a SephadexG-50 Sephadex column, and the component with the largest absorbance at a wavelength of 230nm was collected to obtain the hydrolyzed peptide of limulus plasma protein, which was freeze-dried to obtain the hydrolyzed peptide powder.

Embodiment 3

[0034] (1) heat-treat Limulus blood in a water bath at 90°C for 4 hours, filter it with suction, and wash the denatured protein with distilled water;

[0035] (2) Use a mixture of trypsin and papain to hydrolyze the denatured protein in step (1) to obtain hydrolyzed peptides, centrifuge, and take the supernatant for later use;

[0036] (3) The supernatant was passed through a SephadexG-50 Sephadex column, and the component with the largest absorbance at a wavelength of 230nm was collected to obtain the hydrolyzed peptide of limulus plasma protein, which was freeze-dried to obtain the hydrolyzed peptide powder.

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Abstract

The invention relates to a limuloid plasma protein hydrolysis-peptide with antioxidative activity and a preparation method and application thereof. The preparation method comprises the following steps: a step S1 of causing limuloid plasma to undergo thermostatic waterbath for 2-4 h at 70-100 DEG C, performing suction filtration on the limuloid plasma and using water to wash the limuloid plasma, and reserving the limuloid plasma; a step S2 of using protease to hydrolyze the limuloid plasma obtained through the step S1, and then performing centrifugal separation on the limuloid plasma hydrolysate to obtain supernatant for standby application; a step S3 of causing the supernatant obtained through the step S2 to pass a SephadexG-50 column, and collecting a component with the maximum absorbance at the position of the wavelength of 230 nm, namely obtaining the limuloid plasma protein hydrolysis-peptide. The limuloid plasma is taken as a raw material, the limuloid plasma protein hydrolysis-peptide obtained through hydrolysis has high DPPH free radical scavenging capacity and total antioxidant capacity, and the limuloid plasma protein hydrolysis-peptide is non-toxic and can be prepared into a health care product which maintains beauty and delays senescence. The reutilization of the limuloid plasma is achieved, and the use value of the limuloid plasma is improved.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a limulus plasma proteolytic peptide with antioxidant activity, a preparation method and application thereof. Background technique [0002] The human body is in a dynamic balance of continuous production and elimination of free radicals under normal metabolism, but due to the influence of the external environment, such as solar radiation, inhalation of polluted gases, smoking, etc., this dynamic balance It is easily destroyed. When the balance is disrupted, excessive accumulation of free radicals will not only cause damage to normal tissues in the body, but may also cause some serious diseases such as tumors, heart disease and senile dementia. In order to prevent or reduce the damage caused by free radicals, suitable free radical scavengers are needed to remove excess free radicals in the body and ensure people's health. [0003] Existing free radical scavengers have the disadvanta...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/16A61P39/06
CPCC07K1/16C12P21/06
Inventor 邓春梅杜建喜康信煌何兰珍温燕梅
Owner GUANGDONG OCEAN UNIVERSITY
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