Method for establishing damp-heat diarrhea animal model

Abstract

The invention relates to a method for establishing a damp-heat diarrhea animal model. The method includes the steps of putting a Wistar rat in a metabolism cage and letting the rat freely drink hydromel; alternatively carrying out fasting, sufficient forage feeding, and lard gavage every other day for 10 days; filling a proper amount of liquor on the 10-15th days, and placing the rat in high temperature and humidity environment for 5 days; and injecting Escherichia coli into the abdominal cavity on the 16th day, and injecting again in 24 hours. By observing the general condition of the rat and detecting the blood, blood viscosity, blood biochemistry and serum cytokines, it is shown that the damp-heat diarrhea animal model is established successfully by the method. The animal model constructed by the invention has good reproducibility and stability, and can withstand the test of drug test, so as to provide an ideal animal model for developing new therapeutic drugs.

Description

technical field [0001] The invention relates to the field of Chinese (veterinary) medicine evaluation, in particular to a method for establishing an animal model of damp-heat diarrhea. Background technique [0002] Diarrhea is due to exogenous pathogenic factors or internal injury caused by diet, resulting in dysfunction of the spleen, loss of conduction in the large intestine, internal resistance due to excessive dampness, and the main manifestations are increased stool frequency, loose stools or unresolved grains, even watery samples. Symptoms are very common in Chinese (veterinary) medicine. In recent years, scholars at home and abroad have done a lot of research on animal models of diarrhea in order to study the mechanism of diarrhea and develop drugs for treating diarrhea. The modeling methods mainly include: diarrhea models induced by bacteria, viruses, traditional Chinese medicine, and western medicine. Most of these models ignore the coincidence with traditional Ch...

AI Technical Summary

Problems solved by technology

[0005] Existing animal models of damp-heat diarrhea have the following disadvantages in their modeling methods: (1) Some modeling methods are not close to clinical practice, such as high sugar and high fat, high temperature and high humidity + dextran Sodium sulfate combined with acetic acid, high sugar and high fat, high temperature and high humidity + senna, etc., can simulate the symptoms of damp heat diarrhea, but clinically these reasons cause diarrhea rarely; (2) most of the models use high The method of using sugar and high-fat feed to create "internal dampness" does not include the factors of "disorder of hunger and fullness" that cause damage to the spleen and stomach. This method takes a long time to cause damage to the spleen and stomach, and it is difficult to accumulate enough "internal dampness" in a short period of time. "; (3) Most of the models adopt the method of intragastric administration of Escherichia coli, and the ability of Escherichia coli to cause diarrhea is greatly weakened after being digested by the stomach and in a low pH environment

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