A kind of vaccine adjuvant and its application

A vaccine and adjuvant technology, applied in the field of veterinary biological products, can solve the problems of sterile abscess, affecting the accuracy of test results, easy degradation or denaturation, etc.

A vaccine and adjuvant technology, applied in the field of veterinary biological products, can solve the problems of sterile abscess, affecting the accuracy of test results, easy degradation or denaturation, etc.

CN106177939BActive Publication Date: 2021-01-19PU LIKE BIO ENG
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  • A kind of vaccine adjuvant and its application
  • A kind of vaccine adjuvant and its application
  • A kind of vaccine adjuvant and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation of embodiment 1 antigen

[0051] 1.1 Preparation of porcine circovirus antigen

[0052] According to Chinese patent CN103083655A, porcine circovirus type 2 SH strain virus liquid was prepared and the virus liquid content was measured, and the result: the content of porcine circovirus type 2 SH strain was 5×10 6.0 TCID 50 / ml; and inactivate the virus liquid and detect the inactivation effect with the indirect immunofluorescence method IFA, the result: no green PCV2 positive cells are produced, indicating that the porcine circovirus type 2 SH strain virus liquid is successfully inactivated.

[0053] 1.2 Preparation of Mycoplasma hyopneumoniae antigen

[0054] According to the Chinese patent CN103083655A, the bacterial liquid of Mycoplasma hyopneumoniae HN0613 strain was prepared and the content of bacterial liquid was determined, and the result: the content of Mycoplasma hyopneumoniae strain HN0613 was 1×10 9 CCU / ml; and inactivate and test the bacteri...

Embodiment 2

[0057] The preparation of embodiment 2 adjuvants

[0058] Aluminum glue: purchased from General Chemical, LLC of the United States HPA adjuvant.

[0059] Levamisole mother solution: The levamisole raw material purchased from Xi'an Saino Biotechnology Co., Ltd. was heated and dissolved into a 40 mg / ml solution, and sterilized by filtration to obtain a 40 mg / ml levamisole mother solution.

[0060] Sodium polyacrylate mother liquor: mix 1g sodium polyacrylate purchased from Shanghai Yiji Industrial Co., Ltd. with 100% distilled water, heat and boil until completely dissolved, then cool to room temperature, that is, configure a sodium polyacrylate mother liquor with a concentration of 10mg / ml, and Autoclave at 121°C for 30 minutes before use.

Embodiment 3

[0061] Preparation and animal test of embodiment 3 inactivated vaccine composition

[0062] 3.1 Preparation of inactivated vaccine composition

[0063] The antigen prepared in Example 1 and the adjuvant prepared in Example 2 were diluted with PBS solution, and each antigen solution after dilution was mixed with the final concentration of the adjuvant according to the components contained in the inactivated vaccine composition in Table 1, with 500 Stir at -800 rpm for 10-15 minutes, add 1% V / V of thimerosal (purchased from Sinopharm Chemical Reagent Co., Ltd.) solution before terminating the stirring, so that the final concentration does not exceed 1 / 10,000, fully shake Mix evenly, aliquot, and store at 2-8°C after aliquoting.

[0064]

[0065]

[0066] 3.2 Animal Test of Porcine Circovirus Antigen of Inactivated Vaccine Composition

[0067] Vaccines A1, A2, A3, A6, A7, A9, B1, B2, B3, B6, B7, B9 prepared in Example 3.1 and a reference vaccine control group (PCV2 vaccin...

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Abstract

The invention provides an adjuvant for vaccines, which includes aluminum colloid, levamisole or derivatives thereof, and polyacrylate. The invention also discloses a vaccine composition containing the adjuvant and its application. The adjuvant can not only be used as an adjuvant for inactivated vaccines, but can also be used to prepare protein stabilizing solutions. Vaccine compositions containing this adjuvant can not only cause the body to produce humoral immunity but also cellular immunity. It can also produce a better immune response when the antigen content is low and a single immunization is performed; it can also stabilize the protein without degradation or irreversibility. Variety.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and specifically relates to an adjuvant for enhancing antigen immune response, the adjuvant includes aluminum gel, levamisole or its derivatives, and polyacrylic acid salt, and is used in vaccine compositions and proteins , without producing toxicity or unwanted side effects in the subject. Background technique [0002] Bacterial, viral and parasitic infections are widespread in humans and animals, and diseases caused by these infectious agents are often resistant to antimicrobial therapy, leaving no effective treatment available. Therefore, more and more people use vaccinology to control infection, specifically through vaccination with live pathogens, inactivated pathogens or their products, or genetically engineered pathogen protein subunits to prepare vaccines to induce infection. specific immunity. However, certain antigens do not produce sufficient stimulation of the...

Claims

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Application Information

Patent Timeline
19 Jan 2021
Publication
CN106177939B
IPC
A61K39/39; A61K47/22; A61P37/04
Inventors
张许科; 孙进忠