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A fat graft material containing highly concentrated adipose-derived stem cells and extracellular matrix and its purely physical preparation method and application

A fat-derived, highly concentrated technology, applied in the field of fat transplantation and stem cell therapy, can solve the problems of complex separation process, high cost, limited capacity, etc., and achieve the effect of rich source, low cost, and no rejection.

Active Publication Date: 2019-07-19
鲁峰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there are many limitations in the application of SVF, for example: 1. After transplantation, because SVFs are out of the microenvironment under the physiological state, as a cell suspension, their ability to colonize the target tissue is very limited; 2. The separation process is complicated, And exogenous digestive enzymes need to be added; 3. The extraction process needs to be operated in a specific experimental environment, which is expensive and requires professional technicians

Method used

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  • A fat graft material containing highly concentrated adipose-derived stem cells and extracellular matrix and its purely physical preparation method and application
  • A fat graft material containing highly concentrated adipose-derived stem cells and extracellular matrix and its purely physical preparation method and application
  • A fat graft material containing highly concentrated adipose-derived stem cells and extracellular matrix and its purely physical preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1) Process the obtained fat particles by Coleman technology: centrifuge at 1200g / min for 3 minutes, recover the upper layer of fat after centrifugation, discard the bottom liquid, and transfer the high-density fat tissue in the middle layer to a needle-free syringe, and use scissors The adipose tissue is further shredded.

[0046] The above-mentioned granular fat is obtained by wet liposuction technology, using a liposuction needle to suck fat tissue from the patient's abdomen or thigh.

[0047] 2) Connect the syringe filled with fat in the previous step with a new syringe through the Luer connector (such as figure 1 As shown) Connect the injection ports of the two syringes to achieve a sealed butt joint, push the plunger of the syringe containing adipose tissue, and inject the adipose tissue into the opposite syringe through the connector at a speed of 20ml / s, and continuously push back and forth in this way Note 2min (eg figure 2 shown).

[0048] 3) Take the oil r...

Embodiment 2

[0054] 1) Process the obtained granular fat with Coleman technology: centrifuge at 800g / min for 5 minutes, recover the upper layer of fat after centrifugation, discard the bottom liquid, and transfer the high-density fat tissue in the middle layer to a needle-free syringe, and use scissors The adipose tissue is further shredded.

[0055] The above-mentioned granular fat is obtained by wet liposuction technology, using a liposuction needle to suck fat tissue from the patient's abdomen or thigh.

[0056] 2) Connect the syringe filled with fat in the previous step and a new syringe through the luer connector to connect the two syringe injection ports to achieve a sealed connection, push the syringe piston containing fat tissue, and push the fat tissue through the connector to the In the contralateral syringe, the bolus injection speed is 10ml / s, and the back-and-forth bolus injection is continued for 1min.

[0057] 3) Take the oil retained in step 1) and mix it into the adipose ...

Embodiment 3

[0063] 1) Process the obtained granular fat with Coleman technology: centrifuge at 1200g / min for 5 minutes, recover the upper layer of fat after centrifugation, discard the bottom liquid, and transfer the high-density fat tissue in the middle layer to a needle-free syringe, and use scissors The adipose tissue is further shredded.

[0064] The above-mentioned granular fat is obtained by wet liposuction technology, using a liposuction needle to suck fat tissue from the patient's abdomen or thigh.

[0065] 2) Connect the syringe filled with fat in the previous step and a new syringe through the luer connector to connect the two syringe injection ports to achieve a sealed connection, push the syringe piston containing fat tissue, and push the fat tissue through the connector to the In the contralateral syringe, the bolus injection speed is 20ml / s, and the back-and-forth bolus injection is continued for 1min.

[0066] 3) Take the oil retained in step 1) and mix it into the adipose...

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Abstract

The invention discloses an adipose transplantation material containing highly concentrated adipose-derived stem cells and an extracellular matrix (ECM), and a pure physical preparation method and an application thereof; with application of a fluid vortex principle, most of mature adipocytes in an adipose tissue are broken; at the same time, with application of a flocculation sedimentation technology, oil droplets generated from breaking the adipocytes are gathered and removed. A series of mechanical processing processes are adopted, the pure physical means is adopted, and no any exogenous substances are added. The multiple physical methods are used for removing the mature adipocytes and grease affecting the tissue retention rate after transplantation. In a prepared SVF-gel, the adipose-derived stem cells and endothelial progenitor cells are highly concentrated; the concentrated stromal vascular fraction cells (SVFs) still adhere on the ECM, the number of stem cells fix-planted in local after transplantation is far greater than the ordinary adipose transplantation number; as a stem cell therapy method, the adipose transplantation material has better long-term curative effect.

Description

technical field [0001] The invention belongs to the field of fat transplantation and stem cell therapy, and relates to the technology of rejuvenation after fat facial transplantation and repair of soft tissue damage, specifically a transplant material for enriching stem cells and extracellular matrix after purely physical treatment of granular fat, its preparation method and application . Background technique [0002] Adipose-derived stem / stromal cells (ASCs) and stromal vascular fraction cells (SVFs) are more and more widely used in the field of stem cell therapy, especially the application of SVF has been carried out in clinical trials in many countries. It has obvious advantages in improving the preservation rate after fat transplantation and wound repair: 1. SVFs obtained from freshly isolated fat tissue contain a large number of biologically active autologous ASCs; 2. It comes from autologous fat tissue without immune rejection; 3. There are abundant reserves in the hu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/60A61L27/38A61L27/36
CPCA61L27/36A61L27/3834A61L27/60
Inventor 鲁峰姚尧董自清廖云君张攀马菁晶高建华
Owner 鲁峰