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A kind of extraction method of high purity and high molecular weight chitin

A technology with high molecular weight and extraction method, applied in the biological field, can solve the problems of low purity and molecular weight of chitin, high acid-base wastewater, high equipment requirements, etc., and achieve the effect of high molecular weight, low ash content and high dissolution rate

Active Publication Date: 2018-09-07
英诺世家生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Chinese patent CN104031176A provides a method for extracting chitin from fly maggots. After stirring, soaking and centrifuging the shells of fly maggots with hydrochloric acid, take the solids and add sodium hydroxide aqueous solution, bathe in water at 80-90°C for 20 minutes, take the solids again after centrifugation, and wash them with water. 1. To obtain chitin after drying and pulverizing. This method has many steps, and there are many acid-base wastewater, which requires high equipment, and the purity and molecular weight of chitin obtained are not high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Decalcification: After crushing 10g of insect carapaces, place them in 200g of dilute acid with a mass concentration of 10% and soak for 4 hours at 50°C, filter, wash the filter cake with water for 3-5 times and dry to obtain 7.2g of decalcified Product A;

[0023] (2) Deproteinization: put 7.2g decalcification product A, 0.09g trypsin, 0.09g papain, 0.18g alkaline lipase and 144g water in a three-necked flask, control the pH of the reaction solution to 7.5, and stir at 40°C After hydrolysis for 3 hours, filter, wash and dry the filter cake to obtain 4.3 g of deproteinized product B;

[0024] (3) Purification: 4.3g of deproteinized product B, 28g of hexanoic acid, 22g of oxalic acid, 14g of pyridine and 0.43g of NaCl were stirred and dissolved at 60°C for 3 hours, then filtered, and the filtrate was dried under reduced pressure to remove the solvent, and then passed through NaCl was removed by washing with water, and 3.2 g of chitin was obtained by drying. The HPLC...

Embodiment 2

[0026] (1) Decalcification: After crushing 10g of insect carapaces, place them in 250g of dilute acid with a mass concentration of 15% and soak for 6 hours at 30°C, filter, wash the filter cake with water for 3-5 times and dry to obtain 7.8g of decalcified Product A;

[0027] (2) Deproteinization: put 7.8g decalcified product A, 0.12g trypsin, 0.12g papain, 0.24g alkaline lipase and 78g water in a three-necked flask, control the pH of the reaction solution to 8, and stir at 50°C After hydrolysis for 2.5 hours, filter, wash and dry the filter cake to obtain 5.1 g of deproteinized product B;

[0028] (3) Purification: 5.1g deproteinized product B, 40g hexanoic acid, 32g oxalic acid, 20g pyridine and 1g MgSO 4 , stirred and dissolved at 70°C for 2 hours, filtered, and the filtrate was dried under reduced pressure to remove the solvent, and then washed with water to remove MgSO 4 , dried to obtain 3.8g chitin, the HPLC purity was 93.8%, the recovery rate was 35.6%, and the ash c...

Embodiment 3

[0030] (1) Decalcification: After crushing 10g of insect carapaces, put them in 300g of dilute acid with a mass concentration of 20% and soak for 5 hours at 40°C, filter, wash the filter cake with water for 3-5 times and dry to obtain 6.9g of decalcified Product A;

[0031] (2) Deproteinization: put 6.9g decalcification product A, 0.14g trypsin, 0.14g papain, 0.28g alkaline lipase and water in a three-necked flask, control the pH of the reaction solution to 8.5, stir and hydrolyze at 55°C After 2 hours, filter, take the filter cake and wash and dry to obtain 4.6g of deproteinized product B;

[0032] (3) Purification: 4.6g deproteinized product B, 40g hexanoic acid, 32g oxalic acid, 20g pyridine and 1.4g Na 2 SO 4 , stirred and dissolved at 80°C for 1 hour, filtered, and the filtrate was dried under reduced pressure to remove the solvent, and then washed with water to remove Na 2 SO 4 , dried to obtain 3.5g of chitin, the HPLC purity was 93.1%, the recovery rate was 32.6%, ...

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Abstract

The invention provides an extraction method for high-purity high molecular weight chitin. The method comprises the following steps: (1) crushing insect chitin, adding into dilute acid, digesting for 4-6 hours at 30-50 DEG C, filtering, taking filter cake, washing with water and drying, thereby obtaining a decalcifying product A; (2) putting the decalcifying product A, complex enzyme and water into a reaction vessel, controlling reaction liquid PH at 7.5-8.5, stirring at 40-55 DEG C, hydrolyzing for 2-3 hours, and then filtering, taking the filter cake, washing with water and drying, thereby obtaining a deproteinization product B; (3) putting the deproteinization product B into a mixed solvent, adding neutral salt, dissolving for 1-3 hours by stirring at 60-80 DEG C, filtering, reducing pressure and drying the filtrate, removing solvent, washing with water, removing the neutral salt and drying, thereby obtaining the required chitin. According to the invention, the chitin extraction technique is simple, the device is conventional, the acid-alkali wastewater yield is low, the requirement for green and environment-friendly production is met and the obtained chitin is high in purity, high in molecular weight and low in ash content.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting chitin, in particular to a method for extracting chitin with high purity and high molecular weight. Background technique [0002] Chitin, also known as chitin, chitin, etc., is an important component (about 10-30%) of the shells of many lower animals, especially arthropods such as shrimps, crabs, and insects, and also exists in lower plants such as fungi and algae In the cell wall of fungi, it is a natural high molecular polymer linked by β(1→4) glycosidic bonds from the basic units of 2-acetylamino-2-deoxy-D-glucose, and is the only cationic animal fiber found so far and the only alkaline polysaccharide. Chitin and its derivatives have many unique functions and characteristics, and have been widely used in the fields of environmental protection, food, medicine, making functional materials, agriculture and forestry, light textile industry, etc., a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/003
Inventor 吴冰滢
Owner 英诺世家生物科技(北京)有限公司
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