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Microorganism polysaccharide and its preparation method and application

A microbial polysaccharide and seed technology, applied in the direction of fermentation, can solve the problems of unreported immune activity, high cost, long cycle, etc., and achieve the effects of enhancing immune function, low production cost, and easy operation.

Inactive Publication Date: 2008-05-28
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the direct extraction of polysaccharides from fruiting bodies of wild fungi is strictly limited by natural resources. The production of polysaccharides by artificially cultivating fruiting bodies or liquid fermentation mycelium has a long cycle, low yield, and high cost, resulting in a polysaccharide content of only 0.1g per tube. The price of the injection is 40-200 yuan
(2) Bacterial exopolysaccharides can be industrially produced on a large scale through fermentation. The main products that have been put into production are xanthan gum, thermosetting polysaccharide and gellan gum, etc., but no immune activity has been reported. They are currently mainly used in food and chemical industry etc
(3) It has been reported that exopolysaccharides of bacteria with immune activity can be synthesized, including exopolysaccharides of lactic acid bacteria and Agrobacterium 1202 strains, but they are still in the shake flask test stage, and the yield is low

Method used

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  • Microorganism polysaccharide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Using glucose as carbon source, batch fermentation production test with 10L fermenter

[0040] slant seed culture

[0041] Inoculate the preserved Caragana rhizobium species on the slant medium, and cultivate it in an incubator at 30°C for 18h, wherein the composition of the medium is (in g / L): glucose 5, (NH 4 ) 2 SO 4 0.3, KH 2 PO 4 12H 2 O 0.3, K 2 HPO 4 0.2, yeast hydrolyzed powder 0.5, H 3 BO 3 0.002, Na 2 MoO 4 0.002, agar 20, the balance is water; medium pH 7.0.

[0042] shake flask seed culture

[0043] Insert the slant seeds into the liquid medium, and cultivate them on a shaker at 150r / min for 16h under the condition of 28°C, and use them as seed liquid for later use, wherein the composition of the medium is (in g / L): glucose 10, (NH 4 ) 2 SO 4 0.8, KH 2 PO 4 12H 2 O0.61,K 2 HPO 4 0.39, yeast hydrolyzed powder 1, H 3 BO 3 0.002, Na 2 MoO 4 0.002, the balance is water; the pH of the medium is 7.0.

[0044] 10L fermenter...

Embodiment 2

[0048] Example 2 Using sucrose as carbon source, batch fermentation production test with 10L fermenter

[0049] slant seed culture

[0050] The preserved Caragana rhizobium strains were inoculated on the slant medium, and cultivated in an incubator at 30°C for 20h, wherein the composition of the medium was (in g / L): glucose 7, (NH 4 ) 2 SO 4 0.5, KH 2 PO 4 12H 2 O 0.5,K 2 HPO 4 0.3, NaCl 0.1, yeast hydrolyzed powder 0.75, agar 22, medium pH 7.1, and all the other ingredients are the same as in Example 1.

[0051] shake flask seed culture

[0052] Put the slant seeds into the liquid culture medium, and cultivate them on a shaker at 190 r / min for 17 hours at 30° C., and use them as seed liquid for later use, wherein the composition of the culture medium is the same as that in Example 1.

[0053] 10L fermenter batch fermentation

[0054] Put the seed liquid into the liquid culture medium according to the inoculum amount of 7.5%, and cultivate it in a 10L fermenter. The...

Embodiment 3

[0057] Embodiment 3 takes starch hydrolyzate as carbon source, with 100L fermentor batch fermentation production test

[0058] slant seed culture

[0059] The preserved Caragana rhizobium strains were inoculated on the slant medium, and cultivated in an incubator at 31°C for 24h, wherein the composition of the medium was (in g / L): glucose 10, (NH 4 ) 2 SO 4 0.8, KH 2 PO 4 12H 2 O 0.61,K 2 HPO 4 0.39, NaCl 0.1, yeast hydrolyzed powder 1, agar 23, medium pH 7.2, and all the other ingredients are the same as in Example 1.

[0060] shake flask seed culture

[0061] Put the slant seeds into the liquid medium, and cultivate them on a shaker at 220 r / min for 18 hours at 31° C., and use them as seed liquid for later use, wherein the composition of the medium is the same as that in Example 1.

[0062] seed pot seed culture

[0063] Put the seed liquid into the liquid culture medium of the seed tank according to the inoculation amount of 10%. After 24 hours of cultivation, i...

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Abstract

The microorganism polysaccharide is made with the CCTCC M 205108 Chinese peashrub rhizobium as bacterial, using the carbohydrate as carbon source, and using ammonium salt as nitrogen source. The method comprises the following steps: culturing the slant face seed, culturing shaking bottle seed, culturing seed jug, batch fermentation or supplying material batch fermentation, and extracting polysaccharide. The output of batch fermentation is 7.7g / L-10.2g / L, and the output of supplying material batch fermentation is 15.1g / L-16.2g / L. The molecular weight of the polysaccharide is between 2.7X104Da and 3.0X104Da. According to mouse test, the polysaccharide possesses the action of improving immunity.

Description

technical field [0001] The invention relates to a fermentation method for synthesizing desired compounds, and specifically belongs to a microbial polysaccharide, a production method thereof, and an application of the polysaccharide. Background technique [0002] Biological polysaccharides in nature come from plants, animals and microorganisms. According to their biological functions, they can be divided into storage polysaccharides, structural polysaccharides and polysaccharides with special functions. Many studies have shown that all kinds of polysaccharides play a very important role in life activities. In recent years, active polysaccharides, especially microbial active polysaccharides, have become a hot spot in scientific research and application development. In addition to their biological significance to microorganisms themselves, microbial active polysaccharides, more importantly, their special structures and properties determine their use in It has extensive and impo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C08B37/00
Inventor 赵良启黄晓波韩勇史清亮张建国
Owner SHANXI UNIV
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