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Surfactant recovery technology in lignocellulose hydrolyzate fermentation process

A technology of surfactant and lignocellulose is applied in the field of biomass raw material degradation to produce fuel ethanol through fermentation process, which can solve the problems of complex process, high equipment investment cost, sugar loss, etc., achieve wide application prospects and improve atom utilization rate. Effect

Active Publication Date: 2020-01-14
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, water washing, physical and chemical detoxification consume a lot of water resources, equipment investment costs are high and the process is complicated, and the detoxification effect is poor and the loss of sugar is serious.

Method used

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  • Surfactant recovery technology in lignocellulose hydrolyzate fermentation process
  • Surfactant recovery technology in lignocellulose hydrolyzate fermentation process
  • Surfactant recovery technology in lignocellulose hydrolyzate fermentation process

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Lignocellulose hydrolyzate model substance 20mL, containing glucose concentration 71g.L -1 , mixed inhibitors (phenol, guaiacol, vanillin, HMF, furfural, levulinic acid mixture) (2.0g.L -1 ), add 0.2g.mL -1 PEG-400 (no PEG added in the comparative example), adding yeast cell concentration 0.8*10 8 ~0.96*10 8 per mL, the pH value was adjusted to 4.3, placed in a shaker for shaking, and fermented at 33°C for 48 hours. Solid-liquid separation is carried out after fermentation, and the recovered yeast cells are directly used for the next fermentation. Glucose conversion rate and ethanol concentration were as figure 2 shown. It can be seen from the comparative data in the figure that when PEG is not added to the fermentation system, the glucose is hardly converted, and the yeast cells are almost completely dead, which cannot be recycled and reused. However, when PEG was added to the fermentation system, the glucose was completely converted 100% and obtained 32g.L -1 ...

Embodiment 2

[0033] Lignocellulose hydrolyzate model substance 20mL, containing glucose concentration 71g.L -1 , mixed inhibitors (phenol, guaiacol, vanillin, HMF, furfural, levulinic acid mixture) (2.0g.L -1 ), add 0.2g.mL -1 PEG-1000, add yeast cell concentration 0.8*10 8 ~0.96*108 per mL, the pH value was adjusted to 4.3 with sulfuric acid solution, placed in a shaker for shaking, and fermented at 33°C for 48 hours. After fermentation, solid-liquid separation is carried out, and the upper liquid is distilled, extracted, and re-distilled, and the remaining liquid is surfactant, buffer, and the recovered yeast can be directly used in the next fermentation process (such as figure 1 ), add some new yeast. PEG-1000 and the recovered yeast were recycled 3 times and the results were as follows: image 3 shown. It can be seen from the figure that PEG-1000 can be recycled and reused, and the glucose conversion rate and ethanol concentration remain basically unchanged.

Embodiment 3

[0035] The experimental procedure is the same as in Example 2, except that 0.2g.mL is added -1 PEG-200, glucose conversion rate, ethanol yield and concentration data are shown in Table 1. It can be seen from the table that PEG-200 can be recycled and reused. After three cycles, the glucose is completely converted, and the ethanol concentration remains basically unchanged.

[0036] Table 1 Glucose conversion rate and ethanol concentration data

[0037]

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PUM

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Abstract

The invention provides a technique for recovering surfactants in the fermentation process of lignocellulose hydrolyzate, which belongs to the field of biomass degradation and fermentation to produce fuel ethanol. This technology does not require detoxification treatment for lignocellulose hydrolyzate pretreated by acid hydrolysis or steam explosion, and directly adjusts the pH value, adds surfactants, inserts Saccharomyces cerevisiae for ethanol fermentation, and directly separates and recovers yeast cells; The enrichment of surfactants, fermentation inhibitors, and buffers is achieved simultaneously with ethanol; the coupled separation and recovery of surfactants, buffers, and fermentation inhibitors is realized by extracting fermentation inhibitors. The advantages of the present invention are: not only improves the recycling efficiency of yeast, but also realizes the coupled recovery of surfactants, buffers, and fermentation inhibitors; compared with the process of distilling ethanol, the recovery of surfactants does not increase energy consumption, and recovery The process is simple, effectively reduces pollution and cost, and further promotes the industrialization process of cellulosic ethanol.

Description

technical field [0001] The invention relates to the field of producing fuel ethanol through a fermentation process through the degradation of biomass raw materials, in particular to a surfactant recovery method for detoxifying lignocellulose hydrolyzate. Background technique [0002] With the depletion of fossil energy and the aggravation of environmental pollution, the development and utilization of renewable clean energy fuel ethanol has received widespread attention. Traditional ethanol fermentation uses sugar or starch as raw materials, both of which are the main sources of food. The production of fuel ethanol from grains has already constituted a threat to world food security, and it is imperative to find other raw materials to replace grains. Lignocellulose is the most abundant biomass resource in the world. The production of fuel ethanol from lignocellulose can not only effectively alleviate the energy crisis and reduce environmental pollution, but also meet the susta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J11/08C07C41/34C07C43/11
CPCY02W30/62
Inventor 张宗超刘秀梅
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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