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Method for removing heavy metal in algae

A heavy metal and microalgae technology, applied in microorganism-based methods, biochemical equipment and methods, unicellular algae, etc., can solve problems such as seedling heavy metal poisoning, to ensure stability, solve healthy and normal growth, and solve seedling heavy metal poisoning Effect

Inactive Publication Date: 2016-12-07
TIANJIN OCEAN PAL CAROL BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of this, the present invention aims to propose a method for removing heavy metals in algae, which not only removes the problem of heavy metal content, solves the problem of seedling heavy metal poisoning, but also ensures the stability of heavy metal ions in the water environment and the normality of algae. growth requirements, the present invention is suitable for removing heavy metal ions in large-scale aquaculture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The culture environment of the microalgae is 26.5° C.; the light intensity is 1500 Lux, the dissolved oxygen is 4 mg / L, and the humidity is 20%.

[0021] When the concentration of algae is 1 million algae cells / ml, the concentration of 2 mg / L disodium ethylenediaminetetraacetate is added, and the heavy metal in the water is reduced by 15% after three days.

[0022] For the configuration of microalgae culture solution, add BG11 medium to seawater with a salinity of 30‰, and add the following nutritional components to each liter of seawater: 1.5g of sodium nitrate, 0.04g of potassium dihydrogen sulfate, and magnesium sulfate heptahydrate 0.075g, calcium chloride heptahydrate 0.036g, sodium carbonate 0.02g, citric acid 0.006g, iron citrate 0.006g, boric acid 0.286g, manganese chloride tetrahydrate 0.181g, zinc sulfate 0.0222g, sodium molybdate 0.039g, Copper sulfate pentahydrate 0.0079g, cobalt nitrate hexahydrate 0.005g.

[0023] The algae liquid at this time was taken a...

Embodiment 2

[0026] The culture environment of the microalgae is 27° C.; the light intensity is 2000 Lux, the dissolved oxygen is 5 mg / L, and the humidity is 20%.

[0027] When the concentration of algae is 1 million algae cells / ml, the concentration of 4 mg / L disodium ethylenediaminetetraacetate is added, and the heavy metal in the water is reduced by 20% after three days.

[0028] For the configuration of microalgae culture solution, add BG11 medium to seawater with a salinity of 30‰, and add the following nutritional components to each liter of seawater: 1.5g of sodium nitrate, 0.04g of potassium dihydrogen sulfate, and magnesium sulfate heptahydrate 0.075g, calcium chloride heptahydrate 0.036g, sodium carbonate 0.02g, citric acid 0.006g, iron citrate 0.006g, boric acid 0.286g, manganese chloride tetrahydrate 0.181g, zinc sulfate 0.0222g, sodium molybdate 0.039g, Copper sulfate pentahydrate 0.0079g, cobalt nitrate hexahydrate 0.005g.

[0029] The algae liquid at this time was taken at ...

Embodiment 3

[0032] The culture environment of microalgae is 26.5℃; the light intensity is 1000-3000Lux, the dissolved oxygen is 3---6mg / L, and the humidity is 20%.

[0033] When the concentration of algae is 1 million algae cells / ml, the concentration of EDTA disodium is 10 mg / L, and the heavy metals in the water can be reduced by at least 30% after three days.

[0034] For the configuration of microalgae culture solution, add BG11 medium to seawater with a salinity of 30‰, and add the following nutritional components to each liter of seawater: 1.5g of sodium nitrate, 0.04g of potassium dihydrogen sulfate, and magnesium sulfate heptahydrate 0.075g, calcium chloride heptahydrate 0.036g, sodium carbonate 0.02g, citric acid 0.006g, iron citrate 0.006g, boric acid 0.286g, manganese chloride tetrahydrate 0.181g, zinc sulfate 0.0222g, sodium molybdate 0.039g, Copper sulfate pentahydrate 0.0079g, cobalt nitrate hexahydrate 0.005g.

[0035] The algae liquid at this time was taken at a volume fra...

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Abstract

The invention provides a method for removing heavy metal in algae. The method comprises the following step: when the density of microalgae is up to 0.1-1 million cells / ml, adding disodium edetate, wherein the concentration of the disodium edetate is 2-10mg / L. The method provided by the invention solves the problem of removing heavy metal in algae and solves the problem of heavy metal poisoning of seedlings in aquiculture; the method can simultaneously guarantee stability of heavy metal ions in an aquatic environment and normal growth demands of algae; and the method is suitable for removing heavy metal ions in large-scale aquiculture.

Description

technical field [0001] The invention relates to the technical field of aquaculture, in particular to a method for removing heavy metals in algae. Background technique [0002] Microalgae is an important opening bait for aquatic seedlings. It is rich in protein and amino acids, and has a fast growth rate and a wide variety of resources. However, the algae cultivation process contains a certain amount of heavy metals, which will not affect the growth of algae. However, in the process of using algae to cultivate aquaculture seedlings, the heavy metals contained are likely to cause heavy metal poisoning of the seedlings, resulting in a waste of resources. , increasing the cost. The sodium in the disodium edetate added in this experiment is unstable and easily combines with heavy metal ions in water to form a new complexing agent to avoid poisoning of seedlings. Contents of the invention [0003] In view of this, the present invention aims to propose a method for removing hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 柴英辉孙朦朦于宏周文礼王永苓李连星
Owner TIANJIN OCEAN PAL CAROL BIOTECH
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