CHO (Chinese hamster ovary) cell serum-free protein-free culture medium and preparation method thereof

A protein-free medium, serum-free medium technology, applied in biochemical equipment and methods, culture process, tissue culture, etc., can solve problems such as unfavorable optimization and adjustment of medium, the maximum viable cell density of less than 3.5 million, and complex components.
CN106190950AInactive Publication Date: 2016-12-07BEIJING SL PHARMA +2
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING SL PHARMA
Publication Date
2016-12-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to preparation of a cell culture medium, and particularly provides preparation of a novel serum-free protein-free defined-chemical-component cell culture medium. The culture medium contains multiple amino acids, vitamins, inorganic salts, minor elements, carbohydrates and other supplementary factors. The culture medium is free of any extract or hydrolysate. The culture medium is free of any animal-derived component. The culture medium can well support in-vitro suspension culture of CHO (Chinese hamster ovary) cells, and satisfies the demands for CHO cell culture. The culture medium contains defined chemical components, and is low in cost.
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Description

technical field

[0001] The invention belongs to the field of cell culture, and in particular relates to a serum-free and protein-free medium suitable for large-scale suspension culture of CHO cells and a preparation method thereof. Background technique

[0002] Chinese hamster ovary (CHO) cells are widely used in the production of recombinant protein drugs and antibody drugs, which can provide stable and accurate glycosylation modifications, making the expression products closest to natural protein molecules, with high safety characteristics . Traditionally, the cultivation of CHO cells is accomplished by adding 5-10% fetal calf serum to DMEM / F12 basal medium. In addition to supplying nutrients for cells, serum also provides growth factors necessary for cell proliferation in vitro. However, the use of serum has many disadvantages such as large batch-to-batch variance, easy contamination by mycoplasma and viruses, high cost, unfavorable separation and purification of product...

Claims

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