Primer group and kit for detecting seven kinds of diarrhea viruses through multiple PCR (polymerase chain reaction), and detecting method of seven kinds of diarrhea viruses

A diarrhea virus and detection method technology, applied in the biological field, can solve the problems of long time consumption, poor coverage, expensive instrument platform, etc., and achieve the effect of improving sensitivity

Active Publication Date: 2016-12-07
CHINA NAT CENT FOR FOOD SAFETY RISK ASSESSMENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the problems of expensive instrument platform, time-consuming, poor sensitivity and specificity, low detection rate and poor coverage existing in the existing diarrhea virus detection technology, and provide a new detection primer and method for diarrhea virus

Method used

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  • Primer group and kit for detecting seven kinds of diarrhea viruses through multiple PCR (polymerase chain reaction), and detecting method of seven kinds of diarrhea viruses
  • Primer group and kit for detecting seven kinds of diarrhea viruses through multiple PCR (polymerase chain reaction), and detecting method of seven kinds of diarrhea viruses
  • Primer group and kit for detecting seven kinds of diarrhea viruses through multiple PCR (polymerase chain reaction), and detecting method of seven kinds of diarrhea viruses

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Experimental program
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Embodiment 1

[0090] 1. Primer synthesis:

[0091] According to the sequences shown in Table 3, primers of SEQ ID NO.1 and 3-18 were synthesized.

[0092] Table 3 Primer sequence list

[0093]

[0094]

[0095] Taking the amount of substance as the unit, 1 part by weight of each of the primers of SEQ ID NO.3-18 was mixed with 4 parts by weight of the primer of SEQ ID NO.1 to constitute the primer set of this embodiment. Then the specificity verification and sensitivity verification were carried out on the above primer sets.

[0096] 2. Specificity verification:

[0097] The following 16 unrelated pathogens were selected as simulated interference samples: Yersinia pseudotuberculosis (purchased from China Medical Culture Collection Center, No. 53501), Escherichia coli (purchased from China Medical Culture Collection Center, No. 44101), Enterobacter sakazakii (purchased from China Medical Culture Collection Center, No. 21665), Shigella (purchased from China Medical Culture Collection ...

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Abstract

The invention provides a primer group for detecting seven kinds of diarrhea viruses through multiple PCR (polymerase chain reaction), wherein the primer group comprises primers shown as SEQ ID NO.1 and 3-16. The invention also provides a kit for detecting seven kinds of diarrhea viruses through multiple PCR. The kit comprises a multiple PCR primer group, revertase and DNA (deoxyribonucleic acid) polymerase. Through the technical scheme, the sensitivity, the specificity and the simplicity and convenience of the simultaneous detection on the seven kinds of diarrhea viruses are obviously improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer set and kit for multiplex PCR detection of seven diarrhea viruses and a detection method thereof. Background technique [0002] Diarrhea virus infection is a common disease that endangers human health. In developing countries, it has become the main cause of infant mortality. Among them, rotavirus group A, norovirus type GI, norovirus type GII, Zarovirus, astrovirus, enteric adenovirus, boca virus, etc. are the most common causes of diarrhea. And unlike the diarrhea caused by bacteria and parasites, there is currently no effective drug treatment for viral diarrhea. However, due to the habitual application of antibiotics clinically, not only cannot kill the diarrhea virus, but also kill the normal flora in the intestinal tract, causing flora disorder and prolonging the diarrhea time. In the prevention and control of viral diarrhea, vaccination is the most potential method,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/93
Inventor 吴永宁郭云昌李薇薇王晓艳王雷张志强
Owner CHINA NAT CENT FOR FOOD SAFETY RISK ASSESSMENT
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