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Application of Ascaroside △c9 in Controlling Monochamus alternata and Blocking the Spread of Pine Wood Nematode

A technology for P. pineapple and pine wood nematode, applied in the application, nematicide, insecticide and other directions, can solve the problems such as the inability to form and cut off the spread, the delay of the emergence time of P. It is easy to popularize and apply, has obvious control effect and does not pollute the environment.

Active Publication Date: 2019-03-22
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, interfering with and regulating the development of Monochamus alternatus larvae can delay or prevent the eclosion of Monochamus alternatus, thus making L. Ⅳ Unable to form and cut off the way of its spread

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] In the area where B. xylophilus occurs, before the pupation of Monochamus alternatus begins at the end of April and the beginning of May, 40 Pinus massoniana plants containing multiple monochamus alternatus larvae were selected. The identification method is as follows: pine trees with pine wood nematode damage do not flow fat after drilling, there are traces of monochamus alternatus spawning grooves, and some pine needles lose green and yellow. 20 strains are used as the experimental group, and 20 strains are used as the control group. On the sunny side of the tree body, the xylem monochamus alternata bores into the hole with an electric drill to drill a hole with a diameter of 1 cm and a depth of 2 cm, which communicates with the pupal chamber of the monochamus alternatus larvae. The dose of ascaroside △C9 was directly added to the wells of the experimental group at a dose of 6nM / well, and the dosage of ascaroside △C9 was 30nM / m 3 Pine tree body. In the control group,...

Embodiment 2

[0024] Ascaroside ΔC9 was dissolved in methanol and adsorbed on a rubber septa (0.5 cm diameter, 1 cm length). In the area where B. xylophilus occurs, 40 Pinus massoniana plants containing multiple monochamus larvae were selected. The identification method is as follows: pine trees with pine wood nematode damage do not flow fat after drilling, there are traces of monochamus alternatus spawning grooves, and some pine needles lose green and yellow. 20 strains are used as the experimental group, and 20 strains are used as the control group, and holes with a diameter of 1 cm and a depth of 2 cm are drilled with an electric drill in the xylem of the tree body on the sunny side of the tree. In the experimental group, a number of silicone rubber plugs containing 1 μl of ascaroside △C9 were scattered and inserted into the holes (after mixing 5 μl of ascaroside △C9 and 100 μl of methanol, 20 μl was dropped into the silicone rubber plug, and the ascaroside could be used after the solven...

Embodiment 3

[0026] Ascaroside △C9 was dissolved in ethanol and adsorbed on a silicone rubber stopper (0.5 cm diameter, 1 cm length). In the area where B. xylophilus occurs, 40 Pinus massoniana plants containing multiple monochamus larvae were selected. The identification method is as follows: pine trees with pine wood nematode damage do not flow fat after drilling, there are traces of monochamus alternatus spawning grooves, and some pine needles lose green and yellow. 20 strains are used as the experimental group, and 20 strains are used as the control group, and holes with a diameter of 1 cm and a depth of 2 cm are drilled with an electric drill in the xylem of the tree body on the sunny side of the tree. In the experimental group, a number of silicone rubber plugs containing ascaroside △C9 were scattered and inserted into the holes (take 20 μl of 3 μM ascaroside △C9 ethanol solution and drop it into the silicone rubber plug, and it can be used after the solvent evaporates). The dosage o...

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Abstract

The invention relates to a method for controlling Monochamus ahernatus Hope and intercepting pine wood nematode propagation by using ascaroside deltaC9. The method for controlling Monochamus ahernatus Hope by using ascaroside deltaC9 is characterized in that the ascaroside deltaC9 is placed in pine trees containing larvae of Monochamus ahernatus Hope. The method can be used in control of woodland Monochamus ahernatus Hope and treatment of pine wood nematode infected pine trees to solve the problems of large quantity of Monochamus ahernatus Hope populations, heavy damages and rapid diffusion of pine wood nematodes by Monochamus ahernatus Hope in the outbreak site of the pine wood nematodes.

Description

technical field [0001] The invention relates to the application of ascaroside ΔC9 in the prevention and control of Monochamus alternata and the transmission of pine wood nematode. Background technique [0002] Monochamus alternatus does not occur much in areas where pine wood nematode does not occur, and the damage is not enough to attract people's attention. After the pine wood nematode invaded China, it cooperated with the medium monochamus monochamus, and the population densities of both were greatly increased, forming a trend of rapid expansion and spread. [0003] Pine wood nematode disease, known as the "cancer" of pine trees, is the most dangerous forest disease in the world. It is a devastating disease of pinaceae plants. The host plant can die within 2-3 months after being infected with the disease, and 3- The entire pine forest can be destroyed within 5 years, which is extremely harmful. Since the first discovery of pine wood nematode disease in Nanjing in 1982 i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A01P7/04A01P5/00A01G13/00
CPCA01G13/00A01N43/16
Inventor 赵莉蔺孙江华
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI