Natural fibroin-base macroporous scaffold for bone defect repair and preparation method of macroporous scaffold

Abstract

The invention relates to a natural fibroin-base macroporous scaffold for bone defect repair and a preparation method of the macroporous scaffold. The macroporous scaffold consists of silk fibroin and formylated glucan. The preparation method comprises the following steps: mixing a silk fibroin solution and a formylated glucan solution; and then, injecting a mixture into a die, standing by at 37 DEG C for 24h, taking materials out of the die, and conducting freeze-drying, so that the natural fibroin-base macroporous scaffold for the bone defect repair is obtained, wherein the mass ratio of the silk fibroin to the formylated glucan is at 1:2-20:3. The method disclosed by the invention is simple to operate, free from the application of complex equipment, low in preparation cost, green and pollution-free; the prepared macroporous scaffold is biodegradable in vivo, so that secondary injury on a patient is avoided; and the macroporous scaffold can achieve a better therapeutic effect, and the macroporous scaffold is higher in mechanical strength than a pure-fibroin scaffold.

Description

technical field [0001] The invention belongs to the field of tissue engineering scaffolds and preparation thereof, in particular to a natural silk fibroin-based macroporous scaffold for repairing bone defects and a preparation method thereof. Background technique [0002] Bone tissue engineering uses biological and tissue engineering techniques to plant in vitro cultured seed cells on synthetic or natural extracellular matrix carriers, compound with related inducing factors, and implant them into the body to achieve the purpose of repairing bone defects. The problem of bone defect repair provides a broader application prospect. [0003] Silk fibroin is a natural fibrous protein secreted by non-vertebrate Lepidoptera insects. It has high purity and is harmless to the human body. It not only has good biocompatibility, but also has no allergies and irritations to organisms. coagulation. However, the poor mechanical properties of pure silk fibroin affect its wide application i...

AI Technical Summary

Problems solved by technology

However, the poor mechanical properties of pure silk fibroin affect its wide application in the field of biomedicine. Therefore, compounding with other natural polymer materials is an important means to improve the mechanical strength of materials.

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