A Polyploid Cell Breeding Technique of Euonymus euonymus

Abstract

The invention relates to a polyploidy cell breeding technique for red winged euonymus leaves, belongs to the field of plant ploidy breeding and relates to a method for cultivating a triploid plant by inducing the cells of red winged euonymus leaves. The method comprises the processes of adopting the endosperms of the red winged euonymus leaves for inducing callus and free protoplast and sorting the triploid protoplast under an axenic cultivation condition and inducing and cultivating the triploid protoplast into a triploid plant. The method further comprises the process of eliminating the apical bud dormancy of the triploid red winged euonymus leaves plant. According to the invention, the rich triploid cells are sorted from abundant diploid cells of diploid wild plant seed endosperms and cultivated into the triploid plant and the growth of the triploid plant is boosted.

Description

technical field

[0001] The invention belongs to the field of plant ploidy breeding and relates to a method for engineering breeding of Euonymus euonymus triploid cells. It involves the processes and methods of inducing callus formation from endosperm cells of wild Euonymus euonymus seeds; dissociating protoplasts, staining and ploidy sorting triploid protoplasts; protoplast-induced regeneration of plantlets; terminal bud dormancy relief of plantlets and other processes and methods. Background technique

[0002] Red leaf euonymus ( Euonymus alatus 'Compactus') is a deciduous shrub of the genus Euonymus in the family Euonymus, with green, glabrous branches when young and corky wings on older branches. Flowers light red or light yellow. The leaves are dark green in spring and summer, and turn purple or fiery red in autumn, so it is called "flaming euonymus". As a garden flower and tree, it is planted alone, in rows, or in groups in scenic spots, campuses, courtyards, lakesid...

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