Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method and application of hypocrellin A in inhibiting botrytis cinerea of tomatoes

A technology of tomato cinerea and hypocretin A, applied in the field of microorganisms, can solve the problem of not providing tomato cinerea, and achieve the effects of easy availability and purification of raw materials, high phototoxicity, and high triplet quantum yield

Inactive Publication Date: 2017-01-04
胡莉莉
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But does not provide the method that is applied to inhibiting Botrytis cinerea

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of Hypocretin A (HA) Extract

[0026] Take 50-100g of Zhuhuangzizuo, add 500mL of absolute alcohol and soak for 10-24h, preferably more than 12 hours. Filter the alcohol extract with qualitative filter paper, preferably filter 4 times, evaporate the alcohol under reduced pressure with a rotary evaporator, and concentrate to obtain the HA extract.

[0027] Preparation of HA solution: Take a certain amount of HA extract, add a small amount of absolute ethanol as a solvent, and make HA mother liquor. Measure the absorbance value at 465nm with a UV spectrophotometer, and refer to [Huang Xiaobo. Optimization of the liquid fermentation process of the medicinal fungus Zhuhuang and the extraction of hypocrellin. Zhejiang: Zhejiang Agriculture and Forestry University, 2010] HA standard regression equation, calculate HA content. Dilute the HA mother liquor to 10mg / L, 20mg / L, 40mg / L, 60mg / L with 0.05M PBS buffer solution containing 5% ethanol (except for CAT activity...

Embodiment 6

[0053] Effect of HA on SOD activity of Botrytis cinerea: Weigh 1g mycelium, put it into a 150mL conical flask, add 30mL 40mg. The buffer solution replaced the HA solution as the control group, and the treatment time was set to 0h, 3h, 6h, 12h, 24h, and 36h. Three sets of repetitions were set for each time gradient.

[0054] Analysis of the results: There was no significant difference in the SOD activity of Botrytis cinerea without HA treatment among different treatment times. Using 40mg.L-1 HA to treat Botrytis cinerea, with the prolongation of treatment time, the SOD activity showed a downward trend. After 24h of treatment, the SOD activity was lower, compared with 0h, 3h, 6h, 12h, the difference was significant (P<0.5). At 36h, the activity of SOD decreased to the lowest level, which was 27.7% lower than that of the control group. This shows that HA inhibits the activity of SOD, reduces the activity of SOD, and cannot remove superoxide anions in the cells in time, resulti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of microbes and relates to a method and application of hypocrellin A in inhibiting botrytis cinerea of tomatoes. The method comprises the step of contact of a hypocrellin A solution with botrytis cinerea of tomatoes and / or an object infected with botrytis cinerea of tomatoes; and when the hypocrellin A solution is in contact with the botrytis cinerea of tomatoes and / or object infected with the botrytis cinerea of tomatoes, the botrytis cinerea of tomatoes and / or object infected with the botrytis cinerea of tomatoes is irradiated under light intensity of 12000LX for 10-24h. The invention provides a nontoxic and nuisance-free method for inhibiting the botrytis cinerea of tomatoes.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a method for inhibiting Botrytis cinerea of ​​tomato by hypocrellin A and an application thereof. Background technique [0002] Tomato gray mold is a worldwide disease caused by a plant pathogenic fungus, the pathogen is Botrytis cinerea (B. cinerea) belonging to the subphylum Deuteromycota. The pathogen mainly harms the flowers, fruits, stems and leaves of tomato, causing the fruit and leaves to rot, resulting in a large-scale reduction in tomato production. Generally, the production can be reduced by 20% to 30%, and in severe cases, it can be reduced by 50%. It has spread to my country since the 1980s. . [0003] Chemical control methods have the advantages of wide control range, fast control speed, and simple control operation, so the traditional control methods are still dominated by chemical pesticides. The common chemical pesticides used to control tomato botrytis mainly in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01N35/06A01P3/00
CPCA01N35/06
Inventor 胡莉莉
Owner 胡莉莉
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com