Open cultivation method of sugarcane tissue culture seedling

A technology for open cultivation and tissue culture seedlings, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve problems such as high investment and technical requirements, high production costs, and restrictions on popularization and application, and achieve simplified production operations, Improve work efficiency and improve quality

Active Publication Date: 2017-01-11
SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the strict aseptic operation and culture environment, traditional tissue culture requires high investment and techn

Method used

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  • Open cultivation method of sugarcane tissue culture seedling

Examples

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Example Embodiment

[0025] Example 1

[0026] An open method for cultivating sugarcane tissue culture seedlings includes the following steps:

[0027] 1. Culture equipment and disinfection: culture flasks for conventional tissue culture for induction culture; seedling trays for proliferation and rooting culture. Seedling trays are 57cm long and 37cm wide. Each tray has a mouth width of 5cm in diameter, a bottom width of 2cm in diameter, and a depth of 2cm. 54 holes. It also includes a spray system composed of nutrient liquid tanks, air compressors, time-controlled switches, water pipes, and spray heads. During cultivation, the seedling tray is placed in a plastic tray, which is slightly smaller than the seedling tray. Culture bottles, bottle caps, seedling trays, plastic trays, etc., are soaked in an effective concentration of 0.05% sodium hypochlorite aqueous solution for 7-8 hours before use. The inoculation room of the incubation room is irradiated with ultraviolet light and sterilized with brom...

Example Embodiment

[0035] Example 2

[0036] An open method for culturing sugarcane tissue culture seedlings includes the following steps:

[0037] 1. Culture equipment and disinfection: culture flasks for conventional tissue culture for induction culture; seedling trays for proliferation and rooting culture. Seedling trays are 57cm long and 37cm wide. Each tray has a mouth width of 5cm in diameter, a bottom width of 2cm in diameter, and a depth of 2cm. 54 holes. It also includes a spray system composed of nutrient liquid tanks, air compressors, time-controlled switches, water pipes, and spray heads. During cultivation, the seedling tray is placed in a plastic tray, which is slightly smaller than the seedling tray. Culture bottles, bottle caps, seedling trays, plastic trays, etc. are soaked with an effective concentration of 0.05% sodium hypochlorite aqueous solution for 7-8 hours before use. The inoculation room of the incubation room is irradiated with ultraviolet light and sterilized with bromi...

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Abstract

The invention relates to a plant seedling cultivation method, in particular to an open cultivation method of a sugarcane tissue culture seedling and belongs to the technical field of biology. The open cultivation method of the sugarcane tissue culture seedling comprises: selecting and sterilizing a cultivation apparatus, preparing an antibacterial agent, carrying out induced cultivation of a tissue culture seedling, carrying out proliferation culture, and carrying out rooting culture; tissue culture seedling production is simplified, the antibacterial agent is added only to a culture medium, and inoculation and cultivation can be carried out under bacterial environmental conditions; working efficiency is greatly improved, production cost is reduced, and popularization and application of tissue culture seedlings is facilitated; the breeding process of the tissue culture seedling is open, photosynthetic and respiratory abilities of the tissue culture seedling are enhanced, and the quality of the tissue culture seedling is good; a culture medium nutrient solution is attached to the surface of a tissue culture plant in a sprayed manner, sufficient oxygen is provided for the growth of the tissue culture seedling, and harm of phenolic metabolites to the tissue culture seedling is reduced.

Description

technical field [0001] The invention relates to a method for cultivating plant seedlings, in particular to an open method for cultivating sugarcane tissue culture seedlings, which belongs to the field of biotechnology. Background technique [0002] Sugarcane is an important sugar crop in my country, and its planting area and sugar production account for more than 90% of my country's sugar crops and sugar production. Therefore, the production of sucrose is directly related to the safe supply of sugar in our country. At present, there are many factors affecting the safe production of sucrose, among which the quality of seedlings is one of the main factors affecting the yield and quality of raw material cane. [0003] At present, there is a single variety of sugarcane production in my country, and the new Taiwan sugar series account for about 80% of the total area. The long-term single variety has led to a decrease in the ability of the sugar industry to resist natural risks a...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 李松刘红坚刘俊仙卢曼曼淡明余坤兴刘丽敏刘欣何毅波谭芳谭宏伟杨荣仲王维赞张伟珍陈科义
Owner SUGARCANE RES INST GUANGXI ACADEMY OF AGRI SCI
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