Sweetsop peel volatile oil extract with anti-tumor activity as well as preparation method and application thereof

An anti-tumor activity, lychee peel technology, applied in anti-tumor drugs, medical preparations containing active ingredients, drug combinations, etc., can solve the problem of no anti-tumor active ingredients of custard apple, etc. Good killing effect

Active Publication Date: 2017-01-11
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the anti-tumor active ingredients of the pericarp of custard apple fruit

Method used

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  • Sweetsop peel volatile oil extract with anti-tumor activity as well as preparation method and application thereof
  • Sweetsop peel volatile oil extract with anti-tumor activity as well as preparation method and application thereof
  • Sweetsop peel volatile oil extract with anti-tumor activity as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Preparation of volatile oil from custard apple peel

[0023] Take 20g of dried custard apple peel, grind it into a 500mL ground-mouth flask, add 200mL of distilled water, connect the volatile oil extraction device, heat and extract by steam distillation for 5-8h, collect the obtained volatile oil into a centrifuge tube, and rinse the volatile oil extractor twice with ether , Combine the ether layer and volatile oil, evaporate the ether to dryness under a normal temperature fume hood, add anhydrous sodium sulfate, and store at -20°C.

[0024] 2. Analysis of the volatile oil components of the custard apple peel

[0025] GC-MS analysis conditions are: helium flow rate 1.0mL / min, column temperature is set at 60°C for 4 minutes, raised to 170°C at a rate of 5°C / min, maintained at 170°C for 4 minutes, and then maintained at a rate of 5°C / min Rise to 250°C, maintain at 250°C for 5 minutes, inject 1 μL of sample, the GC-MS total ion chromatogram of litchi peel volatile oil ...

Embodiment 2

[0031] Embodiment 2 Inhibitory effect of anemone fruit peel volatile oil on human tumor cells in vitro

[0032] 1. The killing effect of volatile oil on SMMC-7721 cells was tested by MTT method: 8000 cells were planted in each well of a 96-well plate, and after 24 hours, the volatile oils of different concentrations prepared in Example 1 were added, respectively 3.1 μg / mL, 6.2 μg / mL, 12.5 μg / mL, 25 μg / mL, 50 μg / mL, 100 μg / mL and 200 μg / mL, after 48 hours, add 20 μL of MTT to each well, incubate for 4 hours, remove the medium, add 150 μL DMSO, and microplate reader Detect the absorbance value at 490nm, and calculate the IC50 value for anti-tumor results as follows figure 2 As shown, the above experiment was repeated three times, and the IC50 was 54.81 μg / mL.

[0033] 2. Inoculate SMMC-7721 cells in a 6-well plate, 2*10 per well 5 After 12 hours, different concentrations of volatile oil (20 μg / mL, 40 μg / mL) were administered. After 24 hours, the cells were collected, partly ...

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Abstract

The invention discloses sweetsop peel volatile oil extract with anti-tumor activity. The sweetsop peel volatile oil extract takes peels of sweetsop fruits as raw materials, and volatile oil containing spathulenol (32.51%), palmitic acid (9.41%) and the like is extracted through adopting a volatile oil extracting device. By carrying out a lot of experiments to screen, wastes are changed into valuable things, and the sweetsop peels are used as the raw materials and are screened to obtain volatile oil components with the anti-tumor activity; and an in-vitro MTT experiment shows that the prepared sweetsop peel volatile oil has very good killing activity on tumor cells including liver cancers and the like and has important social benefits and economical effects.

Description

technical field [0001] The invention relates to a plant extract, in particular to a volatile oil extract of anemone pericarp with antitumor activity, a preparation method and application thereof. Background technique [0002] Malignant tumors are frequent and common diseases that seriously endanger human health and life. As early as 2000-3000 years ago, there were records about tumors in Egypt and my country. Among them, liver cancer, which has the highest incidence rate in my country, has become the second most deadly cancer because of its high occultity and poor clinical treatment effect. Among the different treatment methods, chemotherapy, especially interventional therapy (local chemotherapy) is an important means for the treatment of advanced liver cancer, but the cardiotoxicity and nephrotoxicity of chemotherapy drugs seriously endanger the health of patients. The role of human beings is getting more and more attention. [0003] Ansanola is an important plant of the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61P35/00C11B1/10
CPCA61K36/185A61K2236/37A61K2236/53C11B1/10
Inventor 李祥陈亚运陈建伟
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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