Supercharge Your Innovation With Domain-Expert AI Agents!

A fluorescent bioprobe for simultaneously detecting three ions of cu2+, mg2+ and pb2+ and its detection method

A fluorescent biological probe, fluorescence technology, applied in the field of detection, can solve the problems of interfering with the catalytic activity of deoxynuclease, affecting the sensitivity of metal detection, etc.

Inactive Publication Date: 2019-06-14
CHONGQING TECH & BUSINESS UNIV
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing metal detection methods based on deoxynuclease technology can only detect one metal ion, Li L et al [Li L, Feng J, Fan Y, et al.Simultaneous imaging of Zn 2+ and Cu 2+ inliving cells based on DNAzyme modified gold nanoparticles[J] reported that two different deoxynucleases were immobilized on the surface of gold nanoparticles, which can simultaneously detect Zn 2+ and Cu 2+ However, due to the large volume of gold nanoparticles, it will interfere with the catalytic activity of deoxynuclease immobilized on its surface, affecting the sensitivity of metal detection, etc.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A fluorescent bioprobe for simultaneously detecting three ions of cu2+, mg2+ and pb2+ and its detection method
  • A fluorescent bioprobe for simultaneously detecting three ions of cu2+, mg2+ and pb2+ and its detection method
  • A fluorescent bioprobe for simultaneously detecting three ions of cu2+, mg2+ and pb2+ and its detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The preparation of embodiment 1 graphene oxide (GO)

[0059] Weigh 2g of graphite powder and dissolve it in 50mL of concentrated sulfuric acid, stir for 2h; slowly add 10g of potassium permanganate at 20°C, then transfer to a 35°C water bath and stir vigorously for 4h; add 600mL of ultrapure water to dilute the reaction solution; gradually add dropwise 20mL 30%H 2 o 2 solution; the reaction mixture was washed 5 times with 0.1mol / L HCl and ultrapure water, and ultrasonically dispersed for 1h; the GO mixture was centrifuged at 5000rpm for 10min, and the supernatant was taken as GO for the experiment.

Embodiment 2

[0060] Embodiment 2 The effectiveness analysis of fluorescent biological detection system of the present invention

[0061] See image 3 , group 1 is to measure the fluorescence intensity value that only contains single nucleic acid fragment S1, S2 or S3 of the present invention respectively; mixture, heated to 95°C for 5min, and then gradually cooled to room temperature); group 3 is the fluorescence intensity value measured by adding 100ug / mL graphene oxide to group 1; group 4 is the value measured by adding 100ug / mL graphene oxide to group 2 The fluorescence intensity value obtained; group 5 is the fluorescence intensity value measured by adding 100ug / mL graphene oxide after only adding a certain metal in group 2; group 6 is adding 100ug / mL graphene oxide after adding three metals mL of graphene oxide measured fluorescence intensity values.

[0062] As can be seen from the comparison between group 1 and group 2, the fluorescence intensity of the fluorescent bioprobe formin...

Embodiment 3

[0063] Embodiment 3 Selectivity Analysis of the Fluorescent Biodetection System of the Present Invention

[0064] Target ions were added to 100 μM Cu 2+ Concentration, 2000μM mg 2+ concentration and 500nM Pb 2+ , interfering ion 100 μM Mn 2+ , Ca 2+ ,Hg 2+ ,Zn 2+ ,Co 2+ ,Sn 2+ Tris-HCl buffer solution (50mmol / L, pH 7.5, containing 50mmol / L MgCl 2 ) and mix well, incubate at room temperature for 5 min, then add 100 μg / mL GO in Tris-HCl buffer solution (pH 7.5, containing 50 mmol / L MgCl2), mix well, and incubate for 25 min. Fluorescence emission spectra were detected with a fluorophotometer.

[0065] See the experimental results Figure 4 , the system for the target ion Cu 2+ ,Mg 2+ , Pb 2+ Has a good fluorescence signal response, interfering ions (Mn 2+ , Ca 2+ ,Hg 2+ ,Zn 2+ ,Co 2+ ,Sn 2+ ) of the fluorescent signal response is not obvious, therefore, the fluorescent biodetection system provided by the invention has high sensitivity and selectivity, and can d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the detection field and more particularly, specially to a fluorescent biological probe, comprising a biological detection system for the fluorescent bioprobe and a method for detecting the concentration of Cu2 +, Mg2 + and Pb2 +. The fluorescent biological probe comprises a detection system which comprises a fluorescent bioprobe capable of detecting the concentration of Cu2 +, Mg2 + and Pb2 + at the same time. The fluorescent biological probe has the advantages of high sensitivity, simple operation and low cost and can be widely used in the areas as medicine, food safety, clinical and environmental detection.

Description

technical field [0001] The invention belongs to the field of detection, and in particular relates to a method capable of simultaneously detecting Cu 2+ , Mg 2+ and Pb 2+ Fluorescent bioprobe of three ions, biodetection system containing the fluorescent bioprobe and detection method thereof. Background technique [0002] With the development of science and technology, soil, water sources, food, etc. are gradually polluted by industrial waste gas, wastewater, and waste residues. Mercury (Hg 2+ ), copper (Cu 2+ ), lead (Pb 2+ ), magnesium (Mg 2+ ) and other metal pollutants are enriched and accumulated in large quantities, and accumulate in organisms through the food chain, which has brought serious health hazards to people, such as lead (Pb 2+ ), as the most harmful heavy metal to the human body, even at a very low concentration, it also has a serious toxic effect on various systems of the human body, and has a great impact on the growth and development of children, seri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68G01N21/64
CPCG01N21/6486
Inventor 云雯杜云峰吴虹尤琳烽刘兴燕黄昱
Owner CHONGQING TECH & BUSINESS UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com