A kind of preparation method of calcium dibutyryl cyclic adenosine phosphate

Abstract

The invention discloses a calcium dibutyryladenosine cyclophosphate preparation method, which is characterized by comprising: (1) adding adenosine cyclophosphate and calcium hydroxide to water according to a molar ratio of 1:1-1.2, carrying out a reaction for 30-40 min, filtering to remove insoluble matters, adding ethanol having the volume 2-3 times the volume of the filtrate to the filtrate, filtering after a calcium adenosine cyclophosphate salt is precipitated, and carrying out vacuum drying at a temperature of 60-80 DEG C to obtain calcium adenosine cyclophosphate; (2) adding the calcium adenosine cyclophosphate obtained in the step (1) and butyric anhydride to a reaction kettle according to a ratio of 1 g:7-12 ml, protecting with nitrogen, and carrying out a stirring reaction for 1-3 h; and (3) adding 0-4 DEG C water having the volume 3-4 times the volume of the reaction liquid obtained in the step (2) to the reaction liquid, stirring for 30-40 min, carrying out nano-filtration on the reaction liquid, concentrating the trapping liquid, and carrying out freeze drying or spray drying to obtain the calcium dibutyryladenosine cyclophosphate. According to the present invention, the selectivity of the obtained calcium dibutyryladenosine cyclophosphate is more than 90%, the reaction time is short, the reaction process is simple and environmental friendly, and the method is suitable for industrial application.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and specifically relates to a method for preparing cyclic adenosine phosphate calcium from cyclic adenosine monophosphate and performing acylation reaction with butyric anhydride to obtain dibutyryl cyclic adenosine phosphate calcium as a product. Background technique [0002] Cyclic Adenosine-3',5'-Mconophosphate (cAMP for short) is a derivative of nucleotides, which is an important substance in the human body that can transmit nitrogen-containing hormones. When the nitrogen-containing hormone is secreted from a certain cell, it travels to the target cell with the body fluid, and when it acts on the specific receptor on the cell membrane, it activates the adenylyl cyclase in the cell membrane. 2+ or Ca 2+ Under the condition of existence, the adenosine triphosphate (ATP) in the cell is converted into cAMP, and then protein kinase is activated by cAMP, and various enzyme systems are activated...

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Problems solved by technology

[0005] The traditional synthetic route is to make ethylamine salt first, then acylate and then make calcium salt. Not only is the process cumbersome and the yield is low, but also the quality of the product obtained is poor, and solvents with reproductive toxicity such as pyridine are used, which consumes cost, which increases the difficulty of separation

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