Application of lactobacillus reuteri in replacement of feed antibiotic

A technology of Lactobacillus reuteri and antibiotics, applied in application, animal feed, animal feed, etc., can solve the problems of decreased immune function, endogenous infection of the body, influence of drugs, etc., to reduce intestinal inflammatory response and promote repair , the effect of high safety

Inactive Publication Date: 2017-02-01
ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While the use of antibiotics in feed promotes the growth of livestock and poultry, its disadvantages are becoming more and more obvious, including: the emergence and prevalence of drug-resistant strains, causing endogenous infection or superinfection of the body, resulting in the decline of the body's immune function, drug residues in animal products and Affecting human health and the ecological environment through the food chain

Method used

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  • Application of lactobacillus reuteri in replacement of feed antibiotic
  • Application of lactobacillus reuteri in replacement of feed antibiotic
  • Application of lactobacillus reuteri in replacement of feed antibiotic

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1 Lactobacillus reuteri (Lactobacillus reuteri) LR1 freeze-dried bacterial powder preparation preparation

[0026] Prepare MRS broth medium, its components are: casein peptone 10g, beef extract powder 10g, glucose 20g, yeast extract 5g, dipotassium hydrogen phosphate 2g, sodium citrate 2g, magnesium sulfate heptahydrate 0.58g, tetrahydrate Manganese sulfate 0.25g, sodium acetate 5g, Tween-801mL; mix the above ingredients in 1000mL of water, adjust the pH to 2.5 with concentrated hydrochloric acid, autoclave at 121°C for 15min, and put it in a refrigerator at 4°C for later use;

[0027] Inoculate Lactobacillus reuteri LR1 strain into MRS broth medium, culture at 50rpm at 37°C for 24 hours, inoculate into MRS broth medium according to the volume ratio of 4% inoculum, cultivate at 50rpm at 37°C for 24 hours, then 4°C Centrifuge at 10,000rpm for 10min under the same conditions, collect the cells, and then add a lyoprotectant (15-20% skimmed milk powder (m / v) + 5%...

Embodiment 2

[0028] Example 2 Lactobacillus reuteri (Lactobacillus reuteri) LR1 replaces feed antibiotics on weaned piglets anti-diarrhea effect

[0029] 30 weaned boars of 21-day-old Du × (long × large) three-way hybridization were selected for the experiment. The piglets were divided into three groups, namely the control group (basic diet), the antibiotic group (basic diet + antibiotics), and the Luo Lactobacillus reuteri group (basic diet + Lactobacillus reuteri), 10 pigs in each group. See Table 1 for the grouping treatments of the feeding trials. The basal diet was configured according to NRC2012 and national standards to meet the requirements of various nutrients (Table 2). The test period was 28 days. During the experiment period, the piglets had free access to drinking water and added feed at fixed points every day. Keep the sanitation of the pig house, take insulation measures, and record the diarrhea rate of piglets.

[0030] Table 1 Test grouping

[0031] group F...

Embodiment 3

[0039] Example 3 Lactobacillus reuteri (Lactobacillus reuteri) LR1 preparation replaces feed antibiotics and is applied on weaned piglets

[0040] The feeding experiment adopted a single-factor experimental design, and 90 healthy grown-up binary hybrid weaned barrows (initial weight 6.11 ± 0.05kg) were selected at 21 days of age, and were randomly divided into 3 groups, each of which was the control group (basal diet ), antibiotic group (basic diet+antibiotics), and Lactobacillus reuteri group (basic diet+Lactobacillus reuteri), each group had 5 replicates, and each replicated 6 heads. See Table 4 for the grouping treatments of the feeding experiment. The basal diet was configured according to NRC2012 and national standards to meet the requirements of various nutrients (Table 1). The test period is 42 days. Specifically, from the age of 21 days to the end of 63 days. The experiment was carried out in the Nutrition Experimental Pig Farm of the Institute of Animal Science, Gu...

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Abstract

The invention discloses application of lactobacillus reuteri in replacement of feed antibiotic and belongs to the field of microorganisms and application of the microorganisms. The lactobacillus reuteri is obtained through separation from healthy piglets, and therefore safety is high. The bacterial strain is added into antimicrobial-free feed so as to well prevent the piglets from diarrhea after ablactation, and has a good effect on growth promotion. The bacterial strain can obviously promote repair of intestinal mucous membrane damage caused by ablactation of the piglets, and has an immunity-adjusting function. In this way, the lactobacillus reuteri can be used for resisting diarrhea of the piglets after ablactation and promoting growth as a substitude of antibiotics.

Description

technical field [0001] The invention belongs to the field of microorganisms and applications thereof, in particular to an application of Lactobacillus reuteri LR1 in replacing feed antibiotics. Background technique [0002] In modern pig production, piglet diarrhea is the most common cause of serious economic losses. Diarrhea can lead to slow growth of piglets, decreased immunity, intestinal dysfunction, etc., thereby reducing feed utilization and significantly increasing piglet mortality (Fairbrother et al. al., 2005). Diarrhea caused by pathogenic bacteria is the most common disease in weaned piglets (Fairbrother et al., 2005). The main method currently used to control diarrhea caused by pathogenic bacteria in weaned piglets is the use of in-feed antibiotics (Barton, 2000). In the decades since antibiotics were discovered and started to be used on a large scale, they have become common additives to improve livestock performance and prevent diseases (Thacker, 2013). Whil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/195A23K10/18
Inventor 王丽王志林蒋宗勇温晓鹿杨雪芬马现永胡胜兰
Owner ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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