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Activating fluorescent latex microsphere for D-dipolymer fluorescence immunochromatography test card

A fluorescent latex microsphere and fluorescent immunochromatography technology, which is applied in measurement devices, analytical materials, biological tests, etc., can solve problems such as reduced detection sensitivity, uneven dispersion, and chromatography failure, and achieve stable detection results. Accurate, easy-to-use results

Active Publication Date: 2017-02-01
BEIJING ELCOTEQ BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: after drying, the fluorescent latex microspheres of the D-dimer fluorescent immunochromatography detection card are easy to agglomerate and unevenly dispersed, resulting in failure of chromatography or reduction of detection sensitivity

Method used

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  • Activating fluorescent latex microsphere for D-dipolymer fluorescence immunochromatography test card
  • Activating fluorescent latex microsphere for D-dipolymer fluorescence immunochromatography test card
  • Activating fluorescent latex microsphere for D-dipolymer fluorescence immunochromatography test card

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1) Dissolve the surfactant (4.5mg sodium laurylalanine, 4.5mg N-laurylalanine and 3mg sodium stearate) in 0.5M carbonic acid buffer (H value to 9.6), then add in 0.1ml of DMF, 0.1ml of DMF, add N,N'-dicyclohexylcarbodiimide (DCC) 1mg and 0.6mg N-hydroxysuccinimide (NHS) Stir at room temperature for 3 hours.

[0047] 2) Adjust the pH value of 1ml of fluorescent latex microsphere solution containing 1% solids on the amino surface to 9.6 with 0.5M carbonic acid buffer solution of pH 9.6, then add it to step 1), and continue to stir and react for 3 hours. Acquired activated latex microspheres.

[0048] 3) Use a high-speed centrifuge to centrifuge the fluorescent latex microsphere reaction solution at high speed, remove the supernatant of the reaction solution, and redissolve the fluorescent latex microspheres with 1mL microsphere buffer to form a marker working solution.

[0049] Microsphere phosphate buffer preparation: 0.01M pH7.4 phosphate buffer, containing 0.1 wt% BS...

Embodiment 2

[0051] The operation method of this example is similar to that of Example 1, except that the surfactants in step 1) are: 3 mg sodium dodecylalanine, 3 mg N-dodecylalanine and 6 mg sodium stearate.

Embodiment 3

[0053] The operation method of this example is similar to Example 1, except that the surfactant in step 1) is: 4.5 mg sodium dodecylalanine, 4.5 mg N-dodecylalanine and 1.5 mg mg sodium stearate.

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Abstract

The invention discloses an activating fluorescent latex microsphere for D-dipolymer fluorescence immunochromatography test card and application thereof. The preparation method for the activating fluorescent latex microsphere for D-dipolymer fluorescence immunochromatography test card includes the following steps: 1, the surfactant is put into a buffer solution with pH 8-10, then dimethylformamide, N, N'dicyclohexylcarbodiimide and N-H-hydroxy succinimide are added in; 2, the dispersion liquid of fluorescent latex microsphere on the surface of amidogen is taked out, after regulate the pH to 8-10 with the buffer solution, the dispersion liquid is added into the liquid mixture obtained from step 1, stirring and reaction are carried out, after the reaction is finished, the core and the supernatant are removed, and the surface-activated fluorescent latex microsphere is obtained; wherein, the surface activator is a mixture of dodecyl amidogen propionate, N-dodecyl amidogen propionate and stearate, the weight proportion of the three is 0.5-3:0.5-3:1.

Description

technical field [0001] The invention belongs to the technical field of fluorescence immunochromatography, and in particular relates to an activated fluorescent latex microsphere for a D-dimer fluorescence immunochromatography detection card and its preparation and application. Background technique [0002] Fluorescence immunochromatography detection card (reagent card) is widely used in the field of rapid detection because of its fast and convenient characteristics. The analyte antibody is labeled with fluorescent latex microspheres, and the latex microspheres have surface electron repulsion and van der Waals forces in the liquid phase to keep the latex particles in the liquid phase evenly distributed. However, after spraying on the glass cellulose film and drying, as the solution is evaporated to dryness, the surface tension pushes the latex particles infinitely closer together, resulting in the disappearance of the intermolecular repulsion, and the increase of the van der ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/533G01N33/558G01N33/58G01N33/68
CPCG01N33/531G01N33/533G01N33/558G01N33/585G01N33/68G01N2800/226
Inventor 林斯
Owner BEIJING ELCOTEQ BIO TECH
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