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Breeding method of saffron seed ball

A technology of saffron and bulbs, which is applied in the field of plant tissue culture, can solve the problems of many operation steps, complicated procedures, and easy variation, and achieve the effects of accelerating the breeding process, increasing the induction rate, and shortening the induction time

Active Publication Date: 2017-02-08
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, saffron tissue culture generally uses cut bulbs as explant materials to induce callus to redifferentiate into clustered buds. The operation steps are many, the process is complicated, and it is prone to variation.

Method used

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  • Breeding method of saffron seed ball

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Effect test

Embodiment 1

[0039] A method for breeding saffron bulbs, comprising the following steps:

[0040] 1) Selection of explants, disinfection

[0041] Select healthy and plump saffron small bulbs with a weight of <1g, and conduct cold treatment at 4°C for 4 weeks, remove the outer brown film, rinse with water for 1 hour, and use 0.1% mercury chloride on the ultra-clean workbench Disinfect for 10 minutes, rinse with sterile water 5 times, and dry the water for later use.

[0042] 2) Induction culture

[0043] The small seed bulbs prepared in step 1) were cut into 4 parts on average, with the base facing down, and inoculated on the induction medium. After inoculation, the pollution rate was lower than 5%. The induction medium was: MS basic medium, 6-BA 3mg / L, NAA 1mg / L, sucrose 40g / L, hydrolyzed milk protein (LH) 300mg / L, agar 4g / L; pH=5.8; cultivate in the dark for 10 days, then in the light for 10-20 days, Cultivate temperature 25 ℃, humidity 70%, grow cluster buds on the small bulb of cutti...

Embodiment 2

[0050] Embodiment 2 industrialized high-efficiency test tube sphere breeding method

[0051] 1) Selection of explants, disinfection

[0052] Select healthy and plump saffron bulbs with a weight of <1g, and conduct cold treatment at a temperature of 4°C for 6 weeks, remove the outer brown film, rinse with water for 3 hours, and use 0.1% mercuric chloride on the ultra-clean workbench Disinfect for 12 minutes, rinse with sterile water 5 times, dry the water and set aside.

[0053] 2) Induction culture

[0054] The small seed bulbs prepared in step 1) were cut into 2 parts on average, with the base facing down, and inoculated on the induction medium. After inoculation, the pollution rate was lower than 5%. The induction medium was: MS basic medium, 6-BA 2.5mg / L, NAA 1.5mg / L, white sugar 45g / L, hydrolyzed milk protein (LH) 200mg / L, agar 4g / L; pH=5.8; culture in the dark for 10 days, and then in the light for 20 days , the culture temperature is 25°C, the humidity is 70%, cluster...

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Abstract

A breeding method of saffron seed ball comprises the following steps: selection and disinfection of explants, induction of test tube ball, proliferation of test tube ball, and rooting and transplanting of test tube ball. Small saffron seed balls are taken as the explants. The pollution rate is reduced. The induction rate of test tube ball is increased. The callus phase is not needed, and multiple shoots can be induced out. Multiple shoots can be directly induced to obtain test tube seed balls. The induction time is shortened. The test tube seed balls can be applied to multiplication culture. After proliferation, the test tube balls are subjected to rooting. The saffron test tube ball is converted into saffron seed balls in one step, the time of tissue culture is saved, the operation steps are reduced, the excellent properties of original balls are preserved, and the breeding method can be applied to industrial efficient breeding of saffron. The breeding method provides technical support for the development of saffron industry.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for breeding saffron bulbs. Background technique [0002] Saffron, also known as saffron and saffron, belongs to the herbaceous plant of Iridaceae. It is a rare and precious Chinese medicinal material that is currently in short supply in the domestic and foreign markets. It can not only treat various diseases such as epilepsy, postpartum congestion, wet spots, depression, depression, palpitations and madness, but also a water-soluble natural pigment and tonic. It is used as food, beverage, soup, Coloring and flavoring of fragrances and cosmetics. Known as "red gold" and "plant gold", it has extremely high economic value. [0003] With the gradual understanding of consumers' disease prevention and health care functions of saffron, the market demand for saffron is increasing year by year. According to preliminary statistics, the domestic market currently co...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 杨柳燕张永春蔡友铭王艺程
Owner SHANGHAI ACAD OF AGRI SCI
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