Supercharge Your Innovation With Domain-Expert AI Agents!

Separation technique of Spirulina phycocyanin by polyvinylether palmic acid double-water-phase extraction

A polyvinyl ether and phycocyanin technology, applied in the field of food separation engineering, can solve the problems of high price, increase production cost, poor selectivity of separation phycocyanin, etc., achieve high selectivity, low production cost, solve utilization and high The effect of the value problem

Inactive Publication Date: 2017-02-08
JIANGNAN UNIV
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported patented technology of two-phase extraction and separation of phycocyanin for the preparation of algal phycocyanin, such as Chinese patent CN103880950A, uses expensive ionic liquids to form a two-water phase, which increases production costs. CN102993297A, CN101891809A, etc. use commercially available PEG Composition of polymer two-phase aqueous phase, the selectivity of separating phycocyanin is poor, it needs to be used in combination with other extraction methods such as salting out to obtain high-purity products, and the process is cumbersome

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Preparation of spirulina broken wall serum

[0017] Take the dry powder of Spirulina platensis produced in Cibaiian Biological Engineering Co., Ltd., Dongtai City, Jiangsu Province, use deionized water as the extraction agent, and the solid-liquid ratio of algae powder to deionized water is 1:50, and freeze at -20 °C for 4 After one hour, it was thawed at 37°C, and so repeated freezing and thawing 4 times, the thawed cyanobacteria broken wall liquid was centrifuged at 6000 rpm for 10 minutes, and the supernatant was taken as the broken wall supernatant, and the protein concentration in the supernatant was 1.0mg / ml, phycocyanin purity A 620 / A 280 It is 0.72mg / ml.

[0018] (2) Preparation of polyvinyl ether palmitic acid

[0019] In a 1000ml round bottom flask, put 100g of polyethylene glycol PEG-1000 (purchased from Haian Petrochemical Plant), 2g of triethylamine (purchased from Hebei Shijiazhuang Jinna Chemical Co., Ltd.) and 400ml of toluene (purchased from Suz...

Embodiment 2

[0025] (1) Preparation of spirulina wall breaking liquid

[0026] Take fresh spirulina produced in Jiangsu Dafengci Century Biotechnology Co., Ltd., use 50mmol / L phosphate buffer as the extractant, the solid-liquid ratio of spirulina to deionized water is 1:100, and freeze at -10°C After 4 hours, it was thawed at 37°C and repeated freezing and thawing 5 times. The thawed cyanobacteria wall-broken liquid was centrifuged at 6000 rpm for 10 minutes with a centrifuge, and the supernatant was taken as the wall-broken supernatant. The protein concentration in the supernatant was 3.1 mg / ml, and the purity of phycocyanin was A 620 / A 280 is 0.5.

[0027] (2) Preparation of polyvinyl ether palmitic acid

[0028] Put 100g of polyethylene glycol PEG-3000 (purchased from Haian Petrochemical Plant), 4g of triethylamine (purchased from Hebei Shijiazhuang Jinna Chemical Co., Ltd.) and 700ml of toluene (purchased from Suzhou Qianlihang) into a 1000ml round bottom flask Chemical Co., Ltd.)...

Embodiment 3

[0034] (1) Preparation of spirulina wall breaking liquid

[0035] Take the spirulina powder produced in the spirulina base in Chenghaiyuan, Lijiang, Yunnan, use 150mmol / L phosphate buffer as the extractant, the solid-liquid ratio of spirulina to deionized water is 1:150, and freeze at -20°C for 4 hours Finally, thaw at 37°C, and repeat the freezing and thawing process 6 times. The thawed cyanobacteria wall-broken liquid was centrifuged at 6000 rpm for 10 minutes with a centrifuge, and the supernatant was taken as the wall-broken supernatant, and the purity of phycocyanin in the supernatant was A 620 / A 280 was 0.77, and the protein concentration of the broken wall fluid was 4.6mg / ml.

[0036] (2) Preparation of polyvinyl ether palmitic acid

[0037]In a 1500ml round bottom flask, put 100g of polyethylene glycol PEG-4000 (purchased from Haian Petrochemical Plant), 5g of triethylamine (purchased from Hebei Shijiazhuang Jinna Chemical Co., Ltd.) and 800ml of toluene (purchased...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
purityaaaaaaaaaa
recovery rateaaaaaaaaaa
purityaaaaaaaaaa
Login to View More

Abstract

The invention relates to a separation engineering technology of Spirulina phycocyanin by polyvinylether palmic acid double-water-phase extraction, belonging to the technical field of biochemical separation engineering. The technique comprises the following steps: preparing polyvinylether palmic acid; carrying out two-phase extraction on phycocyanin; and carrying out concentration and freeze-drying on the phycocyanin water solution to obtain phycocyanin powder. The protein recovery rate can reach 85-90%. The method is simple: the Spirulina wall breaking solution is directly enriched and separated by using the polyvinylether palmic acid, thereby simplifying the steps of the purification process. The method has high selectivity; and the spectral purity of the separated phycocyanin reaches A620 / A280>3.2. The raw material can adopt Spirulina fresh algae or dry algae powder, thereby providing a high-value large-scale utilization technique of Spirulina resources.

Description

technical field [0001] The invention provides a preparation method of polyvinyl ether palmitic acid, which is used for extracting phycocyanin from spirulina broken wall serum. The invention belongs to the technical field of food separation engineering. Background technique [0002] Spirulina phycocyanin is present in the phycobilisomes of Spirulina. Phycobilisomes are supramolecular light-harvesting pigment complexes unique to red and cyanobacteria. Phycocyanin is bright blue in color and bright in color. It is the first choice of pure natural pigment for food, high-end eye shadow and lipstick. Phycocyanin can also regulate and synthesize a variety of important enzymes required by human metabolism, has obvious anti-oxidation and anti-inflammatory effects, regulates the human immune system, and enhances the function of the immune system. High-purity phycocyanin has strong fluorescence, and it is made of pure natural fluorescent reagents, which are used in research fields s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/795C07K1/14
CPCC07K14/795
Inventor 王峰
Owner JIANGNAN UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com