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Separation method of spirulina phycocyanin via polyvinylether benzoate two aqueous phase extraction

A technology of polyvinyl ether and benzoate, which is applied in the field of preparation of polyvinyl ether benzoate, can solve the problems of high price, increased production cost, and poor selectivity of separating phycocyanin, and achieve high selectivity and production Effects of low cost, solving problems of utilization and high value

Inactive Publication Date: 2017-05-31
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported patented technology of two-phase extraction and separation of phycocyanin for the preparation of algal phycocyanin, such as Chinese patent CN103880950A, uses expensive ionic liquids to form a two-water phase, which increases production costs. CN102993297A, CN101891809A, etc. use commercially available PEG Composition of polymer two-phase aqueous phase, the selectivity of separating phycocyanin is poor, it needs to be used in combination with other extraction methods such as salting out to obtain high-purity products, and the process is cumbersome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] (1) Preparation of spirulina broken wall serum

[0017] Take the dry powder of Spirulina platensis produced in Cibaiian Biological Engineering Co., Ltd., Dongtai City, Jiangsu Province, use deionized water as the extraction agent, and the solid-liquid ratio of algae powder to deionized water is 1:50, and freeze at -20 °C for 4 After one hour, it was thawed at 37°C, and so repeated freezing and thawing 4 times, the thawed cyanobacteria broken wall liquid was centrifuged at 6000 rpm for 10 minutes, and the supernatant was taken as the broken wall supernatant, and the protein concentration in the supernatant was 1.0mg / ml, phycocyanin purity A 620 / A 280 It is 0.52mg / ml.

[0018] (2) preparation of polyvinyl ether benzoate

[0019] Putting 100g polyethylene glycol PEG-1000 (purchased from Haian Petrochemical Plant) and 0.8% sodium hydroxide (purchased from Nanjing Chemical Reagent Co., Ltd.) aqueous solution in the round bottom flask of 1000ml and weight percentage conce...

Embodiment 2

[0025] (1) Preparation of spirulina wall breaking liquid

[0026] Take fresh spirulina produced in Jiangsu Dafengci Century Biotechnology Co., Ltd., use 50mmol / L phosphate buffer as the extractant, the solid-liquid ratio of spirulina to deionized water is 1:100, and freeze at -10°C After 4 hours, it was thawed at 37°C and repeated freezing and thawing 5 times. The thawed cyanobacteria wall-broken liquid was centrifuged at 6000 rpm for 10 minutes with a centrifuge, and the supernatant was taken as the wall-broken supernatant. The protein concentration in the supernatant was 3.1 mg / ml, and the purity of phycocyanin was A 620 / A 280 is 0.5.

[0027] (2) preparation of polyvinyl ether benzoate

[0028] In the round bottom flask of 1500ml, put into 100g polyethylene glycol PEG-3000 (purchased from Hai'an Petrochemical Plant) and concentration by weight percentage and be 1.8% sodium hydroxide (purchased from Nanjing Chemical Reagent Co., Ltd.) aqueous solution, sodium hydroxide a...

Embodiment 3

[0034] (1) Preparation of spirulina wall breaking liquid

[0035] Take the spirulina powder produced in the spirulina base in Chenghaiyuan, Lijiang, Yunnan, use 150mmol / L phosphate buffer as the extractant, the solid-liquid ratio of spirulina to deionized water is 1:150, and freeze at -20°C for 4 hours Finally, thaw at 37°C, and repeat the freezing and thawing process 6 times. The thawed cyanobacteria wall-broken liquid was centrifuged at 6000 rpm for 10 minutes with a centrifuge, and the supernatant was taken as the wall-broken supernatant, and the purity of phycocyanin in the supernatant was A 620 / A 280was 0.77, and the protein concentration of the broken wall fluid was 4.6mg / ml.

[0036] (2) preparation of polyvinyl ether benzoate

[0037] In the round bottom flask of 1500ml, put into 100g polyethylene glycol PEG-3000 (purchased from Hai'an Petrochemical Plant) and concentration by weight percentage and be 2.5% sodium hydroxide (purchased from Nanjing Chemical Reagent C...

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Abstract

The invention discloses a separation method of spirulina phycocyanin via polyvinylether benzoate two aqueous phase extraction, and belongs to the technical field of food separation engineering. The separation method comprises following steps: polyvinylether benzoate is prepared; two aqueous phase extraction of spirulina phycocyanin is carried out; an obtained spirulina phycocyanin aqueous solution is subjected to condensation and freeze drying so as to obtain spirulina phycocyanin powder. Protein recovery rate ranges from 70 to 90%. The separation method is simple; polyvinylether benzoate is used for enrichment and separation of spirulina wall-broken liquid directly; purification process is simplified; selectivity is high; the spectral purity A<620> / A<280> of spirulina phycocyanin obtained via separation is larger than 3.7; fresh spirulina or dried spirulina powder can be taken as a raw material; and the separation method is capable of realizing spirulina resource high value large-scale utilization.

Description

technical field [0001] The invention provides a preparation method of polyvinyl ether benzoate, which is used for extracting phycocyanin from spirulina broken wall serum. The invention belongs to the technical field of food separation engineering. Background technique [0002] Spirulina phycocyanin is present in the phycobilisomes of Spirulina. Phycobilisomes are supramolecular light-harvesting pigment complexes unique to red and cyanobacteria. Phycocyanin is bright blue in color and bright in color. It is the first choice of pure natural pigment for food, high-end eye shadow and lipstick. Phycocyanin can also regulate and synthesize a variety of important enzymes required by human metabolism, has obvious anti-oxidation and anti-inflammatory effects, regulates the human immune system, and enhances the function of the immune system. High-purity phycocyanin has strong fluorescence, and it is made of pure natural fluorescent reagents, which are used in research fields such a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/795C07K1/14C08G65/332
CPCC07K14/795C08G65/3326
Inventor 王峰
Owner JIANGNAN UNIV
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