Method for removing aflatoxin B1 (AFB1) by use of fusarium and enzyme produced by fusarium

A technology of aflatoxin and Fusarium, applied in the field of microorganisms, can solve the problems of rare and difficult biodegradable compounds

Pending Publication Date: 2017-02-15
石家庄北科盛安生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

AFB1 has a cyclic structure and is a difficult biodegradable compound. Currently, there are few studies on its biodegradation

Method used

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  • Method for removing aflatoxin B1 (AFB1) by use of fusarium and enzyme produced by fusarium
  • Method for removing aflatoxin B1 (AFB1) by use of fusarium and enzyme produced by fusarium
  • Method for removing aflatoxin B1 (AFB1) by use of fusarium and enzyme produced by fusarium

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preparation example Construction

[0044] The preparation method for the bacterial agent that is used to remove the aflatoxin B1 in the biomass material that the third aspect of the present invention relates to, it comprises:

[0045] Step B, inoculating the fermentation strain into the fermentation medium for fermentation culture to obtain a fermentation culture;

[0046] In step C, the fermentation culture is concentrated and separated, and then cleaned and separated to obtain Fusarium wet bacteria (that is, the bacterial agent used to remove aflatoxin B1 in the biomass material);

[0047] Wherein, the fermented strains are obtained from corresponding bacterial strains through seed cultivation.

[0048] As known to those skilled in the art, at present, 18S Ribosomal RNA (18S rRNA) is often used internationally for molecular identification of bacteria, so 18S rRNA can be used for comparison of similarities to obtain their homology. Therefore, the fermentation strains used in the present invention are not limi...

Embodiment 1

[0100] Embodiment 1: preparation is used to remove the bacterial agent of aflatoxin B1 in the biomass material

[0101] (1) Prepare the fermentation medium, put 100ml of the prepared liquid fermentation medium into a 500ml triangular flask, seal the bottle mouth with a parafilm, sterilize it at high temperature (121°C) and high pressure (0.15MPa) for 20min, and then place it on a clean bench Use after 20 minutes of internal ultraviolet irradiation sterilization.

[0102] Described fermenting medium comprises the following components in 1L of water in 1L of water:

[0103]

[0104]

[0105] In some embodiments of the present invention, a solution of 40% (weight) sodium hydroxide and 36% (v / v) hydrochloric acid is used to adjust the initial pH value of the fermentation medium to 7.2.

[0106] (2) Seed cultivation, picking the monoclonal colonies of Fusarium solani R30 strain provided by the present invention and inoculating them into 10ml of fermentation broth, cultivatin...

Embodiment 2

[0112] Embodiment 2: Adopt Fusarium solani R30 to remove aflatoxin B1 in corn

[0113] Take by weighing 50g initial AFB1 content and be 20mg / kg, the cornflour that water content is 16.7%, the wet thalline that makes in the embodiment 1 of 0.1g is added in the cornflour, put into airtight plastic belt after fully mixing, Cultured statically at a temperature of 30°C.

[0114] On the 0th, 2nd, 4th, 6th, 8th, and 15th day, 4 grams of samples were taken respectively, of which 2g of corn flour was placed in a 50mL Erlenmeyer flask, 10mL of ethanol was added to vibrate and extracted for 1h, filtered through a 0.22u inorganic filter membrane, and nitrogen was placed at 60°C Blow dry to 2ml for HPLC determination of AFB1 concentration. The water content of another 2g sample was measured by the 130°C timed drying method, which was used for the conversion of AFB1 concentration in the final corn.

[0115] Kinetic process of Fusarium solani R30 biodegradation of AFB1 in maize image 3 s...

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Abstract

The invention relates to a method for removing aflatoxinn B1 (AFB1) by use of fusarium and an enzyme produced by the fusarium. A fusarium solani R30 microbial inoculum obtained by fermentation culture of fusarium solani R30 and a fusarium solani R30 enzyme preparation obtained by ultrasonic disruption of fusarium solani R30 cells both can be used for efficient degradation of the AFB1 in biological materials. The fusarium solani R30 microbial inoculum and the fusarium solani R30 enzyme preparation can achieve the purpose of rapid, safe and efficient bio-degradation removal of the AFB1 in biological materials. The method is mild in degradation conditions for the AFB1, the optimum reaction temperature is 30 DEG C, and the product quality may not be damaged.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a method for removing aflatoxin B1 by using Fusarium and the enzyme produced therefrom. Background technique [0002] Aflatoxin has strong toxicity, strong teratogenicity and strong mutagenicity, and is one of the most harmful mycotoxins. It not only pollutes food and feed, but also seriously endangers the health of humans and animals. Among the aflatoxins, aflatoxin B1 (Aflatoxin B1, referred to as: AFB1) is the most common and most harmful, and has already brought huge economic losses to related industries. The General Administration of Quality Supervision, Inspection and Quarantine stipulates that AFB1 in food is a mandatory inspection item. China's food hygiene standards stipulate the maximum allowable standards in several major contaminated foods: corn, peanuts, and peanut oil ≤ 20μg / kg; edible oil ≤ 10μg / kg ; other grains, beans, fermented food ≤ 5μg / kg. [0003] Afl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23L5/20C12R1/77
CPCC12N1/14C12N1/145C12R2001/77
Inventor 杨俊明闫海张治涛刘晓璐齐增虎吕乐胡彦卿尹春华许倩倩张海洋
Owner 石家庄北科盛安生物科技有限责任公司
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