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Bacillus atrophaeus BA-7 used for preventing and treating clubroot of cruciferae crops

A technology of Bacillus atrophaeus and cruciferous crops, applied in the direction of chemicals for biological control, methods based on microorganisms, bacteria, etc., can solve uneven control effects, hidden dangers to the environment and human and animal health and safety, and biocontrol strains If there are too few problems, it can achieve excellent control effect, broaden the range of bacteria species, and achieve good control effect

Active Publication Date: 2017-02-15
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First of all, the existing biocontrol strains against cruciferous clubroot are too few, and the control effects are uneven; secondly, the application dose of biocontrol agents is too much, and there are potential environmental and human and animal health and safety hazards; the most important Unfortunately, the genus of the existing bacterial strains is too single, mainly concentrated on Bacillus subtilis (such as Bacillus subtilis XF-1 and Bacillus subtilis M3) and Bacillus amyloliquefaciens (such as Bacillus amyloliquefaciens Bam22), whether there are other effective Biocontrol strains for the prevention and treatment of cruciferous clubroot is a major research topic for researchers in this field

Method used

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  • Bacillus atrophaeus BA-7 used for preventing and treating clubroot of cruciferae crops

Examples

Experimental program
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Effect test

Embodiment 1

[0016] (1) Cultivation of strains

[0017] After culturing Bacillus atrophaeus BA-7 on a solid medium, pick a single colony, place it in a liquid medium, and cultivate it with shaking at 30-32°C for 12 hours at a speed of 220rpm, as a seed solution; the liquid medium The ingredients include by mass percentage: 0.3% of yeast extract, 0.2% of beef extract, 0.8% of peptone, 0.3-0.6% of NaCl, the balance is water, pH=7.0-7.2;

[0018] Put the resultant of step 1) into a fermenter for 24 hours of fermentation, the tank temperature is 30° C., and the stirring speed of the fermenter is 250 rpm; the composition of the culture medium in the fermenter includes by mass percentage: soybean powder 4.5%, peptone 0.12% , glucose 0.6%, starch 2.5%, manganese sulfate 0.1%, ferrous sulfate 0.06%, dipotassium hydrogen phosphate 0.2%, calcium carbonate 0.1%, defoamer 0.01%, the balance is water, pH=7.0-7.4.

[0019] (2) Biocontrol effect test

[0020] Dilute the culture obtained in step (1) by ...

experiment example 1

[0033] Farmland: Rapeseed field;

[0034] A row of fungal agent spraying devices are installed on the straw harvester for spraying the fungal agent to the farmland while harvesting the straw.

[0035] Harvest and crush the straw in the farmland, and apply the Bacillus atrophaeus BA-7 bacterial agent to the farmland, and the application rate is 2 trillion bacteria / mu. The bacterial agent is Bacillus atrophaeus BA-7 fermentation liquid, and the bacterial concentration is 1×10 9 cfu / ml.

[0036] After 45 days of treatment, the straw weight loss rate (full name straw biomass weight loss rate) was detected, and the weight loss rate was 61.3%.

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Abstract

The invention provides bacillus atrophaeus BA-7 used for preventing and treating clubroot of cruciferae crops. The bacillus atrophaeus BA-7 is characterized in that the classification name of the bacillus atrophaeus is Bacillus atrophaeus BA-7, and the bacillus atrophaeus BA-7 is preserved in the China Center for Type Culture Collection (CCTCC) on Dec 21, 2015, and is assigned with the accession number of CCTCC NO:M2015765. Under the low concentration, the bacterial strain has the obvious prevention and treatment effects for the clubroot of the cruciferae crops, and the prevention and treatment effects of a 1000-fold diluent (obtained after culture for 24h) for the clubroot of oilseed rape achieve 87.2%

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a bacillus atrophaeus BA-7 used for preventing and treating clubroot of cruciferous crops. Background technique [0002] Clubroot is a disease prevalent in crops, especially vegetables, and is caused by the infection of Plasmodiophora brassicae Woron. In my country, it mainly harms cruciferous vegetables such as rapeseed, Chinese cabbage, green vegetables and mustard greens. After the crops are infected by Plasmodium, smooth or cracked rough tumors of different sizes are formed on the roots. The aboveground part of the plant grows slowly and wilts due to lack of water. Clubroot is mainly transmitted by dormant spores in the soil through diseased seedlings of vegetable plants, fungus soil carried by water flow, etc. Acidic and moist soil is conducive to the germination and invasion of germs. In recent years, vegetable clubroot has a tendency to spread...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P3/00C12R1/07
CPCC12N1/205C12R2001/07
Inventor 刘勇伍文宪黄小琴张蕾尹全刘红雨周西全
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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