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Culture medium for detecting fungi carried by rice seeds

A rice seed and culture medium technology, applied in the direction of microorganism-based methods, microorganism measurement/inspection, and separation of microorganisms, can solve the problems of small number of fungi, long colony diameter, difficult separation, etc., and achieve the effect of not being easily contaminated by bacteria

Active Publication Date: 2017-02-15
YUNNAN LINPENG AGRI SCI & TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to overcome the existence of isolated fungi in the prior art of seed health detection, which are susceptible to bacterial interference, so that the number of isolated fungi is small, and the fungi carried by the seeds cannot be fully reflected, and when separated, the growth rate of some fungi is relatively slow. Fast, long colony diameter, interference to the isolation of other fungi, and the defect that it is difficult to isolate fungi alone

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  • Culture medium for detecting fungi carried by rice seeds
  • Culture medium for detecting fungi carried by rice seeds
  • Culture medium for detecting fungi carried by rice seeds

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Experimental program
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Effect test

Embodiment 1

[0025] The concrete steps that detect rice seed with substratum of the present invention are as follows:

[0026] (1) The preparation of the medium of the present invention, according to the formulation of the medium of the present invention, each component is weighed and placed in a conical flask, then 1L of water is added, after boiling for 15 minutes, according to the damp heat sterilization method, at 121° C., 0.15 MPa, damp heat is extinguished Bacteria 20min, sub-package in 9cm diameter petri dish for subsequent use (if not being used for the time being, can stay in the triangular flask, sub-pack again after next time sterilization); The formula of culture medium of the present invention is: add peptone (Peptone ) 5g, glucose (Glucose) 10g, potassium dihydrogen phosphate (KH 2 PO 4 )1g, MgSO 4 ·7H 2 O 0.25g, agar (Agarpowder) 15g, Bengal red (Tetrachlorofluorescein) 0.033g, chloramphenicol (Chloramphenicol) 0.1g, copper sulfate (CuSO 4 ) 0.5g, zinc nitrate (Zn(NO 3 ...

Embodiment 2

[0034] Embodiment 2 except being the Yuanyang red rice (Moon Valley) rice seed that gets in addition, all the other experimental methods are identical with embodiment 1, and experimental effect sees for details figure 2 and Figure 4 .

[0035] figure 2 Among them, 2 kinds of fungi were detected in the culture medium of the present invention, ( figure 2 As shown in A, figure 2 In A, 1 is a fungus of the genus Penicillium (Penicillium), and 2 is a fungus of the genus Pythium. See the picture of the spores of the isolated fungi Figure 4 ), the diameter of the fungal colony is 2cm to 3cm, which is convenient for picking and observing. Two kinds of fungi were detected in PDA medium ( figure 2 As shown in B, figure 2 In B, 3 is a fungus of the genus Pythium, and 4 is a fungus of the genus Penicillium. See the picture of the spores of the isolated fungi Figure 4 ), the fungal colonies almost covered the petri dish, the growth of the two fungi overlapped, and it was di...

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Abstract

The invention discloses a culture medium for detecting fungi carried by rice seeds. Each liter of sterilization water of the culture medium contains 5 g of peptone, 10 g of glucose, 1 g of monopotassium phosphate, 0.25 g of MgSO4.7H2O, 15 g of agar, 0.033 g of rose Bengal, 0.1 g of chloramphenicol, 0.05 g of CuSO4, 0.029 g of Zn(NO3)2 and 0.0001 g of 1,5-pentanediamine. The culture medium is improved from a rose Bengal culture medium, more fungi carried by rice seeds can be detected by the aid of the culture medium, the operation is convenient, the fungi cannot be contaminated by bacteria easily, and the diameter of fungus colonies is smaller than that of fungus colonies cultured by a PDA culture medium in the prior art, so that separation and statistics of the fungi are facilitated.

Description

technical field [0001] The invention belongs to the technical field of seed quality detection, and in particular relates to improving the Bengal red culture medium for detecting fungi carried by rice seeds. Background technique [0002] At present, seed health testing has become a routine measure in the process of seed "quality assessment" and "phytosanitary inspection" in many countries, and has become an indispensable part of the seed quality control system. Some countries have established special seed health inspections. Assay laboratory. Seed health measurement is to determine whether the seeds carry pathogenic bacteria (such as fungi, bacteria and viruses), harmful animals (such as nematodes and pests) and other health conditions, that is, to detect the types and quantities of diseases and insect pests carried by seeds, and the detection of fungi carried by seeds It is an important content in the detection of seed health. For example, since the establishment of the Se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04C12N1/02C12R1/80C12R1/66C12R1/845C12R1/77C12R1/645
CPCC12N1/02C12Q1/045
Inventor 李小林徐雨然谷安宇邓伟张锦文谭静安华
Owner YUNNAN LINPENG AGRI SCI & TECH CO LTD