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Detection and evaluation of miRNA markers for strength training effects or combinations thereof and applications thereof

An endurance training, mir-208b technology, applied in the field of miRNA markers or their combination, can solve the problems of sample preservation, intra-assay and inter-assay variation, lack of muscle damage, poor repeatability, etc., to save cost and time, and avoid complicated Detection, easy-to-detect effects

Active Publication Date: 2019-12-06
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, despite gains in strength and skeletal muscle hypertrophy after long-term strength training, these hormones do not change significantly at rest
In addition, the following problems exist in the detection of hormones: First, there are many factors that affect the concentration of hormones in the blood, including storage of samples, intra-assay and inter-assay variability, etc.
Second, some techniques used to detect hormones are poorly reproducible(5)
In addition, some studies have suggested that strength training can cause skeletal muscle cell damage. Studies have speculated that these damage markers may play an important role in the process of exercise-induced skeletal muscle hypertrophy, but muscle damage is usually lacking in the development of hypertrophy (6)

Method used

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  • Detection and evaluation of miRNA markers for strength training effects or combinations thereof and applications thereof
  • Detection and evaluation of miRNA markers for strength training effects or combinations thereof and applications thereof
  • Detection and evaluation of miRNA markers for strength training effects or combinations thereof and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Example 1: Screening of specific miRNA expression profile of strength training program

[0048] (1) The research subjects are healthy young men without strength training experience. All subjects have no neuromuscular, metabolic, hormone-related and cardiovascular diseases. During the whole experiment, all subjects did not take drugs and maintained similar living and eating habits. Forty-five subjects were randomly divided into 3 groups, 15 people in each group (see Table 1 for details of volunteers), and each performed one of muscle endurance training, muscle hypertrophy training, and muscle maximum strength training programs. Each group of strength training includes 5 exercises, namely, bench press, half squat, sitting pull-down, over head lift and standing dumbbell curls. The quiet state before training is used as a control. The subjects were forbidden to perform corresponding strength training after 3 days of physical exercise. The entire strength training lasted about...

Embodiment 2

[0068] Example 2: TaqMan probe Real-time PCR method to measure miRNA in plasma and determine the specific change of miRNA as a biomarker for evaluating the effect of strength training

[0069] Use miRNA-specific reverse primers for reverse transcription to obtain cDNA (product of ABI) that contains a common stem-loop structure but belongs to a specific miRNA. The cDNA reverse reaction system of miRNA is: 2μl RNA, 2μl 5×AMV buffer, 1μl10mmol various dNTP mixtures (Takara company), 0.5μl AMV (Takara company) and 1μl miRNA mature body reverse transcription primer (provided by American ABI company, dedicated For miRNA reverse transcription), use 3.5μl DEPC water to make up the volume to 10μl. The Real-time PCR reaction was subsequently performed, and it was found that the two miRNAs (miR-208b and miR-532) had significant differences before and after muscle endurance training ( figure 2 ), 6 miRNAs (miR-133a, miR-133b, miR-206, miR-21, miR-181a and miR-221) have significant differenc...

Embodiment 3

[0080] Example 3: Statistical analysis of the relationship between specific changes of miRNA in plasma after muscle endurance training and traditional markers of hormones and muscle damage markers

[0081] In order to further verify the feasibility of the three specific miRNAs after strength training as biomarkers for evaluating the effect of strength training, the correlation between miRNAs and 8 traditional strength training biomarkers was analyzed. Correlation analysis found that the levels of miR-532 immediately after muscle endurance training, 1 hour and 24 hours after training have a significant correlation with IL-10 and IGF-1 ( Figure 5 ). Immediately after muscle maximum strength training, the level of miR-133a has a significant correlation with the values ​​of cortisol and testosterone / cortisol ( Image 6 ).

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Abstract

The invention discloses miRNA markers for detecting and evaluating effect of strength training or a combination thereof and use thereof. The miRNA markers or the combination of the miRNA markers comprise / comprises miR-208b, MiR-532, miR-133a, miR-133b, miR-206, miR-21, miR-181a and miR-221, and the combination can be used for detection and / or forecast of the effect of muscle endurance training, muscle hypertrophy training and muscle biggest strength training. Plasma is relative easy to obtain, no other tissue is needed, the detection belongs to non-invasive examination, plasma miRNA can reflect whole body system-wide suffered mechanical, hormonal and metabolic stress during the strength training, the plasma miRNA as an assistant detection index can improve the accuracy level of the detection, can reflect the physiological status of the whole process of the strength training at the molecular level, and provides a new index for the detection of the effect of the strength training and design of strength training programs.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and relates to miRNA markers or combinations thereof for detecting and evaluating strength training effects and applications thereof. Background technique [0002] The quality and function of skeletal muscle play a key role in preventing diseases (such as type 2 diabetes, cardiovascular disease, muscle atrophy, etc.), improving the quality of life, and improving athletic ability (1-4). Strength exercise is the most effective way to improve and maintain the quality and function of skeletal muscles. In the past 30 years, the popularity rate of strength training has increased rapidly. In daily exercise practice, different kinematic parameters (strength training) can be selected according to different purposes (improving skeletal muscle endurance, increasing skeletal muscle hypertrophy, and enhancing muscle maximum strength). Methods, training intensity and amount, choice of exercise methods and sequence, re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/113
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 陈熹崔淑方马继政殷鑫王成
Owner NANJING UNIV
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