Test strip kit for detecting duck-derived materials in food and feed and application of test strip kit

A component detection and detection test paper technology, which is applied in the field of test strip kits for duck source component detection, can solve problems such as difficult to distinguish, difficult to implement, and result discrimination obstacles, and achieve high specificity, accurate results, and improved detection sensitivity. Effect

Inactive Publication Date: 2017-02-15
BEOSON JIANGSU FOOD SAFETY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Various animal-derived ingredients mixed in feed ingredients are easily damaged during high-temperature treatment and processing, and the protein sequences of different species of animals have little difference and are difficult to distinguish. The invention patent "SDS-PAGE method to identify meat and meat products Animal-derived ingredients in China" (publication number: CN102213720A) provides a method for identifying animal proteins from pigs, cattle, sheep, chickens, and fish by means of SDS-PAGE electrophoresis of proteins, but the electrophoresis profiles of proteins from different sources and processing methods Different belts, which bring obstacles to the judgment of results, are more difficult to implement for animal ingredients in feed

Method used

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  • Test strip kit for detecting duck-derived materials in food and feed and application of test strip kit
  • Test strip kit for detecting duck-derived materials in food and feed and application of test strip kit

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Embodiment 1

[0020] In an embodiment of the present invention, a test strip kit for detecting duck-derived components in food and feed, including a primer set and a nucleic acid detection test strip with a nucleotide sequence as shown in SEQ ID No.1-2; the primer set The nucleotide sequence is upstream primer 5'-TGTTACAGGAAGTTACTCGCCT-3', as shown in SEQ ID No.1, downstream primer 5'-GCGGAAGATACAAAAAGACACT-3', as shown in SEQ ID No.2; the 5' of this primer set The ends are labeled separately for easy detection, specifically, two different methods of labeling can be selected from biotin molecules, fluorescein isothiocyanate or digoxin. The substrate of the nucleic acid detection test strip is provided with a sample pad, an absorbent pad and a conjugate pad in sequence; the sample pad is composed of a lateral chromatography matrix, a detection strip embedded in the lateral chromatography matrix, and a quality control strip. The chromatographic matrix is ​​nitrocellulose membrane or nylon mem...

Embodiment 2

[0048] The sensitivity of the nucleic acid test strip detection method, the PCR template is 1 / 10, 1 / 10 2 , 1 / 10 3 , 1 / 10 4 , 1 / 10 5 , 1 / 10 6 , 1 / 10 7 , 1 / 10 8 , 1 / 10 9 , 1 / 10 10 After dilution, the PCR system was established for amplification.

[0049] 1 Materials and methods Duck genomic DNA

[0050] 1.2 Primer design

[0051] 1.3 PCR amplification system

[0052] PCR reaction system, including 10×PCR buffer 10 μL, upstream primer 1.8 μL, downstream primer 1.8 μL, HS TaqDNA polymerase 0.5 μL, DNA template 4 μL, ddH 2 O 1.9 μL, a total of 20 μL.

[0053] PCR reaction conditions: pre-denaturation at 95°C for 5 min, 1 cycle; denaturation at 94°C for 30 sec, annealing at 60°C for 1 min, extension at 72°C for 30 sec, amplification for 30 cycles; extension at 72°C for 10 min, storage at 4°C.

[0054] Take 5 μL each for agarose gel electrophoresis. The gel electrophoresis conditions are: 1×TBE buffer, voltage 100V, electrophoresis time 30min. At the same time, take 5 μ...

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Abstract

The invention disclose a test strip kit for detecting duck-derived materials in food and feed and an application of the test strip kit. The kit comprises a primer group comprises a nucleotide sequence as shown in SEQ ID No.1-2 and a nucleic acid test strip, wherein a sample pad, an absorbent pad and a conjugate pad are arranged on a substrate of the nucleic acid test strip in sequence; the sample pad comprises a lateral chromatography substrate as well as a detection band and a quality control band which are embedded in the lateral chromatography substrate, the lateral chromatography substrate is a nitrocellulose membrane or a nylon film, an antibody of a second nucleotide sequence is fixed on the detection band, and an antibody capable of being bound with an antibody with a specific species source is fixed on the quality control band; colloidal gold granules or latex granules of an antibody or a ligand of a first nucleotide sequence is fixed on the conjugate pad. The kit is easy and quick to operate, high in sensitivity and specificity and low in price.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a test strip kit for detection of duck source components in food and feed and its application. Background technique [0002] The European Union and the United States enacted regulations prohibiting the addition of ruminant-derived protein to feed for ruminants in 1994 and 1997, respectively, and in 2000 the European Union extended this ban to prohibit feeding processed mammals to animals intended for food , bird and fish protein ingredients. The "Administrative Measures for the Safety and Hygiene of Animal-derived Feed Products" promulgated by the Ministry of Agriculture of my country in 2004 stipulates that the use of animal-derived feed products (except milk and dairy products) in ruminant feed is prohibited, but artificially mixed with ruminant The phenomenon of animal (mainly cattle, sheep) meat and bone products occurs from time to time. [0003] Duck-derived materia...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6888
Inventor 周朱晨张根义胡彬张进吴念绮朱倩倩
Owner BEOSON JIANGSU FOOD SAFETY TECH CO LTD
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