Reagent kit for detecting avian leukemia virus J sub-groups

An avian leukemia virus and kit technology, applied in the field of molecular biology, can solve the problems of ALV-J gene becoming faster, more difficult to detect, unsuitable for detection, etc., and achieve the effects of improving detection efficiency, convenient operation and high specificity
CN106399588AActive Publication Date: 2017-02-15CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2017-02-15

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Abstract

The invention provides a reagent kit for detecting avian leukemia virus J sub-groups (ALV-J), and belongs to the technical field of RT-PCR (reverse transcription-polymerase chain reaction) detection. The reagent kit contains a pair of specific primers. Nucleotide sequences of the specific primers are respectively shown as SEQ ID NO.1-2. The invention further provides a method for detecting the avian leukemia virus J sub-groups. The reagent kit and the method have the advantages of high specificity, sensitivity and efficiency, good universality and low cost. Besides, quick differential diagnosis can be carried out on clinical disease samples in 6.5 h, technical means can be provided for early quick diagnosis on the ALV-J and conduction of molecular epidemiological investigation, and accordingly avian leukemia prevention and control can be effectively guided during poultry raising production.
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Description

technical field

[0001] The invention relates to the field of molecular biology, in particular to a kit for detecting the J subgroup of avian leukosis virus and its application. Background technique

[0002] Avian Leukosis (AL) is a general term for a class of neoplastic diseases mainly caused by malignant proliferation of hematopoietic cells caused by Avian Leukosis virus (ALV). ALVs were divided into 10 subgroups from A to J according to the range of hosts, differences in viral envelope antigens, virus interference tests and genome molecular biological characteristics. Avian Leukosis Virus Subgroup J (ALV-J) is a new type of avian leukosis virus isolated from broiler chickens for the first time in 1991, and its prototype strain is HPRS103. ALV-J mainly causes myeloid neoplasia (ML) and various other malignant tumors in broilers. Almost all types of chickens are susceptible to ALV-J. In recent years, ALV-J infection is the main infection in my country, accounting for 65-7...

Claims

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