Boar seminal fluid long-acting diluting powder and application thereof

A pig semen, long-acting technology, applied in the field of pig breeding, can solve the problems of short warranty period, complicated preparation method, pollution, etc., and achieve the effect of saving the amount of breeding

Active Publication Date: 2017-02-22
武汉品格饲料有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention of application number 201210238501.4 closely related to the present invention discloses a long-acting pig semen dilution powder and its preparation method. The storage time of pig semen

Method used

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  • Boar seminal fluid long-acting diluting powder and application thereof
  • Boar seminal fluid long-acting diluting powder and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A long-acting diluted pig semen powder, which is formed by mixing the following components by weight: 4.80 g of glucose, 5.80 g of trisodium citrate dihydrate, 2.18 g of citric acid monohydrate, 7.00 g of ethylenediaminetetraacetic acid, and sodium bicarbonate 5.25g, potassium chloride 2.25g, tris 6.50g, cysteine ​​0.20g, trehalose 2.00g, antibiotic enrofloxacin 0.40g and polymyxin sulfate 0.06g.

[0020] (1) The raw materials of the examples are of analytical grade, weighed, vacuum-packed and sealed to obtain diluted powder, which is stored at 2-8°C.

[0021] (2) When using dilution powder, stir with double distilled water heated to 35-37°C, mix evenly, and set the volume to 1000ml to obtain semen dilution, which is used for dilution of pig semen after 1 hour.

[0022] (3) After the pig semen is collected, use a sperm density meter to test the density of the pig semen with normal smell and color, and then check the sperm motility under a 100-fold microscope on a prehea...

Embodiment 2

[0029] An optimized long-acting dilution powder of porcine semen is formed by mixing the following components by weight: 5.06g of glucose, 6.12g of trisodium citrate dihydrate, 2.30g of citric acid monohydrate, 7.38g of ethylenediaminetetraacetic acid, Sodium bicarbonate 5.54g, potassium chloride 2.37g, tris 6.85g, cysteine ​​0.21g, trehalose 2.11g, antibiotic enrofloxacin 0.42g and colistin sulfate 0.06g.

[0030] (1) The raw materials of the examples are of analytical grade, weighed, vacuum-packed and sealed to obtain diluted powder, which is stored at 2-8°C.

[0031] (2) When using dilution powder, stir with double distilled water heated to 35-37°C, mix evenly, and set the volume to 1000ml to obtain semen dilution, which is used for dilution of pig semen after 1 hour.

[0032] (3) After the pig semen is collected, use a sperm density meter to test the density of the pig semen with normal smell and color, and then check the sperm motility under a 100-fold microscope on a pre...

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Abstract

The invention belongs to the field of swine reproduction, and relates to boar seminal fluid long-acting diluting powder and an application thereof. The boar seminal fluid long-acting diluting powder is prepared from, by weight, formula 1, which comprises 4-6 g of glucose, 5-7 g of trisodium citrate dihydrate, 2-3 g of citric acid monohydrate, 7-8 g of edetic acid, 5-6 g of sodium bicarbonate, 2-3 g of potassium chloride, 6-7 g of tris(hydroxymethyl) aminomethane, 0.1-0.3 g of cysteine, 2-3 g of trehalose, 0.3-0.5 g of antibiotic enrofloxacin and 0.05-0.07 g of polymyxin B standard solution; formula 2 (optimized), which comprises 5.06 g of glucose, 6.12 g of trisodium citrate dehydrate, 2.3 g of citric acid monohydrate, 7.38 g of edetic acid, 5.54 g of sodium bicarbonate, 2.37 g of potassium chloride, 6.85 g of tris(hydroxymethyl) aminomethane, 0.21 g of cysteine, 2.11 g of trehalose, 0.42 g of antibiotic enrofloxacin and 0.06 g of polymyxin B standard solution.

Description

technical field [0001] The invention belongs to the technical field of pig reproduction. It specifically relates to a long-acting dilution powder of pig semen and an application thereof. Background technique [0002] Pig artificial insemination technology is a breeding method that uses artificial equipment to collect boar semen, inspects the quality and dilutes the semen, and then injects the semen into the reproductive tract of estrous sows with equipment to replace the natural mating of boars. In the 1980s It is rapidly popularized all over the world. At present, in Europe and North America, where it is widely used, the penetration rate has exceeded 80%. It is an important means for scientific pig raising and modernization of pig production. It has the following advantages: (1) Saving boars, and reduce the number of times each boar is used, overcome time and regional differences, and can conveniently and timely breed; (2) save manpower, material resources, and financial r...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0226
Inventor 宋忠旭雷彬孙华李良华梅书棋彭先文吴俊静武华玉乔木李明波董斌科刘贵生黄藏玉胡华
Owner 武汉品格饲料有限公司
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