Propolis flavone, propolis flavone refining method and application of propolis flavone

A technology of propolis flavonoids and refining methods, which is applied in applications, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of low total flavonoid content, strong viscosity, and unknown active ingredients, and achieve high total flavonoid content and dosage Small, low-viscosity effect

Active Publication Date: 2017-02-22
上海沪郊蜂业联合社有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for refining propolis flavonoids in order to overcome the shortcomings of existing propolis ethanol extracts such as relatively low total flavonoid content, strong viscosity, and unknown active ingredients.

Method used

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  • Propolis flavone, propolis flavone refining method and application of propolis flavone
  • Propolis flavone, propolis flavone refining method and application of propolis flavone
  • Propolis flavone, propolis flavone refining method and application of propolis flavone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The refining method of propolis flavonoids, its steps are as follows:

[0037] 1) Freezing and pulverizing the propolis, and sieving to obtain propolis powder;

[0038] 2) Add water to the propolis powder, fully oscillate and mix, and add NaHCO with a mass percentage concentration of 3%. 3 Aqueous solution, adjust the pH value to 10, fully oscillate and mix at 75-100°C for 40 minutes, then cool and stand, extract with a separatory funnel, stand still, and separate the extract to obtain the first aqueous phase and the first solid phase;

[0039] 3) Add water to the first solid phase, fully oscillate and mix, add NaOH aqueous solution with a mass percentage concentration of 0.1%, adjust the pH value to 11, fully oscillate and mix at 75-100°C for 45 minutes, then cool and stand, divide Liquid funnel extraction, standing still, the extract liquid is separated to obtain the second aqueous phase and the second solid phase, and the second solid phase is discarded;

[0040] 4...

Embodiment 2

[0049] The refining method of propolis flavonoids, its steps are as follows:

[0050] 1) Freezing and pulverizing the propolis, and sieving to obtain propolis powder;

[0051] 2) Add water to the propolis powder, fully oscillate and mix, and add NaHCO with a mass percentage concentration of 5%. 3 Aqueous solution, adjust the pH value to 9, fully oscillate and mix at 75-100°C for 60 minutes, then cool and stand, extract with a separatory funnel, stand still, and separate the extract to obtain the first aqueous phase and the first solid phase;

[0052] 3) Add water to the first solid phase, fully oscillate and mix, add NaOH aqueous solution with a mass percentage concentration of 0.2%, adjust the pH value to 10, fully oscillate and mix at 75-100°C for 40 minutes, then cool and stand, divide Liquid funnel extraction, standing still, the extract liquid is separated to obtain the second aqueous phase and the second solid phase, and the second solid phase is discarded;

[0053] 4)...

Embodiment 3

[0063] The refining method of propolis flavonoids, its steps are as follows:

[0064] 1) Freezing and pulverizing the propolis, and sieving to obtain propolis powder;

[0065] 2) Add water to the propolis powder, fully oscillate and mix, and add NaHCO with a mass percentage concentration of 8%. 3 Aqueous solution, adjust the pH value to 11, fully shake and mix at 75-100°C for 30 minutes, then cool and stand, extract with a separatory funnel, stand still, and separate the extract to obtain the first aqueous phase and the first solid phase;

[0066] 3) Add water to the first solid phase, fully oscillate and mix, add NaOH aqueous solution with a mass percentage concentration of 0.4%, adjust the pH value to 9, fully oscillate and mix at 75-100°C for 60 minutes, then cool and stand, divide Liquid funnel extraction, standing still, the extract liquid is separated to obtain the second aqueous phase and the second solid phase, and the second solid phase is discarded;

[0067] 4) Pre...

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Abstract

The invention discloses a propolis flavone refining method. The propolis flavone refining method includes the steps of 1) subjecting propolis to freezing and smashing to obtain propolis powder; 2) adding water into the propolis powder, shaking for mixing, adding an NaHCO3 (sodium bicarbonate) solution, adjusting the pH (potential of hydrogen) value, fully shaking for full mixing, cooling, standing, extracting by a separating funnel, standing and separating extracted liquid into a first water phase and a first solid phase; 3) adding water into the first solid phase, shaking for mixing, adding an NaOH (sodium hydroxide) solution, adjusting the pH (potential of hydrogen) value, fully shaking for full mixing, cooling, standing, extracting by the separating funnel, standing and separating extracted liquid into a second water phase and a second solid phase; 4) precipitating out a first flavone compound and a second flavone compound from the first water phase and the second water phase by acid; 5) drying the first flavone compound and the second flavone compound respectively by steaming, and combining to obtain propolis flavone. The invention further discloses the propolis flavone prepared by the propolis flavone refining method and application of the propolis flavone. The propolis flavone refining method has the advantage that propolis flavone content is larger than 75%.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of active ingredients in natural products. More specifically, the present invention relates to a method for refining propolis flavonoids, propolis flavonoids and applications. Background technique [0002] Propolis is a resin that bees collect from plant sprouts, bark and trunk cracks, and mixed with their mandibular gland secretions and beeswax to process a colloidal solid with an aromatic smell. Propolis has various biological effects such as preventing wound infection, regulating immunity, anti-tumor, lowering blood fat, lowering blood sugar and resisting pathogenic microorganisms. Propolis has become one of the most popular health care products in the market in the past 30 years. [0003] The chemical composition of propolis is complex, mainly including the following categories: resins, which account for 50% to 55% of propolis, are the main part of propolis; aromatic oils an...

Claims

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Application Information

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IPC IPC(8): A61K35/644A61P39/06A23L21/20A23L33/00
CPCA61K35/644A23V2002/00A23V2200/30A23V2300/14
Inventor 孙德官
Owner 上海沪郊蜂业联合社有限公司
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