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Preparation method of total flavonoids in psoralea corylifolia and new medical application of total flavonoids in psoralea corylifolia

A technology of psoralen and total flavonoids, which can be applied in the direction of medical formulas, medical preparations containing active ingredients, drug combinations, etc., and can solve problems such as unsuitable for long-term application

Inactive Publication Date: 2017-02-22
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that coumarin components are the main active components of psoralen for the treatment of vitiligo, while bakuchiol, a monophenolic component with a high content in psoralen, shows nephrotoxicity in animal models, so it is not suitable for use in the treatment of vitiligo. long-term clinical application

Method used

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  • Preparation method of total flavonoids in psoralea corylifolia and new medical application of total flavonoids in psoralea corylifolia
  • Preparation method of total flavonoids in psoralea corylifolia and new medical application of total flavonoids in psoralea corylifolia
  • Preparation method of total flavonoids in psoralea corylifolia and new medical application of total flavonoids in psoralea corylifolia

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Experimental program
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preparation example

[0019] Preparation of total flavonoids from psoralen

[0020] According to the polarity difference between the coumarins contained in the psoralen and the monoterpene phenolic components and the flavonoid components, the method adopts a solvent method to separate and purify the total flavonoids of the psoralen, and the method is simple, economical and efficient. The economic advantage of the present invention is that all extraction solvents can be recycled, petroleum ether and ethyl acetate can be recycled conveniently, and the ethanol used in the impurity removal step can be prepared and recycled after density measurement, the cost is low, and it is suitable for industrial production.

[0021] Step 1: Pulverize the psoraleae medicinal material, add 10 times the volume (w:v) of petroleum ether to soak overnight, heat and reflux for extraction twice, each time for 1 hour.

[0022] Step 2: Take the degreased medicinal material powder, add 12 times the volume (w:v) of the original ...

experiment example 1

[0026] Determination of Main Components of Total Flavonoids of Psoraleae

[0027] The present invention adopts high-performance liquid chromatography to detect and quantify 11 main components in the traditional Chinese medicine Psoraleae and the total flavonoids of Psoraleae prepared by the present invention, and use this as an investigation index of the preparation process and a quality control method of the finished product,

[0028] details as follows:

[0029] (1) Total flavonoids of psoralen for test: Accurately weigh total flavonoids of psoralen, dissolve in methanol to prepare a solution with a concentration of 1 mg / mL, pass through a 0.45 μm microporous membrane, and take the subsequent filtrate to obtain total flavonoids The test solution.

[0030] (2) Reference substance: 11 chemical reference substances, including psoralen, isopsoralen, new psoralen isoflavone, psoralen, psoralen, psoralen , isopsoralen chalcone, psoralen cinnin, bakuchiol A, psoralen chalcone, ba...

experiment example 2

[0039] Multi-target in vitro anti-AD activity analysis of main components of psoralen

[0040] In our previous study, we used multiple drug targets such as BACE1, Aβ42, GSK3β, AChE, etc. to screen the activity of nearly 70 commonly used traditional Chinese medicines related to the clinical treatment of AD, and found that the methanol extract of psoraleae had the same effect on the above targets. It showed strong inhibitory activity. In order to determine the main active ingredients of psoralen, this experiment carried out the in vitro anti-AD activity analysis of the above targets for the 11 main components contained in psoralen, and the results proved that the flavonoids of psoralen It is the main active site of its anti-AD effect in vitro.

[0041] The test product: 11 chemical reference substances, including psoralen, isopsoralen, new psoralen isoflavone, psoralen, psoralen, psoralen, isoflavone Psoralen Chalcone, Psoralen Cinning, Bakuchiol A,

[0042] Psoralen chalcone ...

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Abstract

The invention discloses a method for preparing total flavonoids from traditional Chinese medicine psoralea corylifolia and a new application of the total flavonoids in psoralea corylifolia to preparation of drugs for preventing or treating the AD (alzheimer's disease). According to different polarities of main ingredients contained in the psoralea corylifolia, low-polarity monoterpene phenols with toxic and side effects are removed through petroleum ether degreasing, flavonoids are extracted with ethyl acetate, the total flavonoids in the psoralea corylifolia are obtained through impurity removal and refinement with activated carbon, and the purity can reach 50% or higher. The method is economical, efficient and suitable for industrial production. In-vitro activity research confirms that the main flavonoids contained in the psoralea corylifolia have different degrees of inhibition activity for multiple related targets of the AD; animal experiments prove that the total flavonoids in psoralea corylifolia can remarkably improve neurodegeneration memory loss symptoms of AD model mice through oral medication, have no toxic and side effects after long-term medication and can be used for preparing drugs for preventing or treating the AD.

Description

technical field [0001] The invention relates to a preparation method of total plant flavonoids and its new medical application in the field of preventing or treating Alzheimer's disease. Background technique [0002] Alzheimer's Disease (AD), also known as senile dementia, is a neurodegenerative disease with frequent occurrence in the elderly. The incidence rate is about 5% among the elderly over 65 years old, and the incidence rate gradually increases with age. The incidence rate reaches 30-40% in the elderly population over 85 years old. Patients suffering from this disease are due to the progressive death and loss of memory-related neurons, which leads to progressive decline in memory and cognitive ability. Generally, it takes about 8-10 years since the onset to develop severe dementia, often accompanied by bedsores, fractures, Pneumonia, malnutrition and other secondary physical diseases or organ failure lead to death. Due to the complex pathogenic mechanism of the dis...

Claims

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Application Information

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IPC IPC(8): A61K36/487A61P25/28
CPCA61K36/487A61K2236/15A61K2236/33A61K2236/35A61K2236/39A61K2236/53
Inventor 张英涛杨雁芳陈芝婧胡莹
Owner PEKING UNIV
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