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Grub polysaccharide component and preparation method and application thereof

A grub and polysaccharide technology, which is applied to the field of grub polysaccharide components and their preparation, can solve problems such as differences in structural characteristics, and achieve the effects of narrow molecular weight distribution range, clear physical and chemical characteristics and monosaccharide composition.

Active Publication Date: 2017-02-22
HAINAN HONZ PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the polysaccharides found to inhibit tumor vascular metastasis are significantly different from the grub polysaccharides in their structural characteristics.

Method used

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  • Grub polysaccharide component and preparation method and application thereof
  • Grub polysaccharide component and preparation method and application thereof
  • Grub polysaccharide component and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Weigh 10kg of dried grub medicinal material, crush it into medium powder; reflux extract with 40000mL of absolute ethanol for 2 hours (skim off), filter out the ethanol extract, process the grub residue once more according to the above method, filter out the ethanol extract, and the residue After evaporating ethanol to dryness at 40°C, reflux extraction with 60,000 mL of 0.1 mol / L NaOH aqueous solution for 3 times, 2 hours each time. The combined water extracts were concentrated to 10,000 mL by a rotary evaporator at 60°C under reduced pressure. After cooling to room temperature, 3 times the volume of absolute ethanol was added. After standing at 4°C for 12 hours, centrifuge at 3,000g for 15 minutes to collect the precipitate. The precipitate was dissolved in 4000 mL of water, and the freeze-thaw was repeated 8 times, and then the Sevag method (i.e., V 多糖溶液 :V 氯仿 :V 正丁醇 =20︰4︰1 Mix and shake for half an hour, centrifuge, let stand to separate layers, take the water la...

Embodiment 2

[0059]Weigh 1kg of dried grub medicinal material, crush it into a coarse powder; reflux extract with 8000mL 95% ethanol for 2 hours, filter out the ethanol extract, evaporate the dregs to dry with ethanol, dry at 50°C, and reflux extract with 8000mL 0.1mol / L NaOH aqueous solution 3 times, 3 hours each time. The water extracts were combined and concentrated to 800mL under reduced pressure with a rotary evaporator at 65°C. After cooling to room temperature, 2 times the volume of absolute ethanol was added. After standing at 4°C for 18 hours, centrifuge at 3500g for 15 minutes to collect the precipitate. Dissolve the precipitate in 300 mL of water, freeze and thaw repeatedly 10 times, centrifuge and discard the precipitate; then decolorize with 30% aqueous hydrogen peroxide solution 1000 times the weight of the sample at 85°C and reflux for 2 hours. The aqueous solution was dialyzed against distilled water for 24 hours to remove small molecular impurities using a dialysis membran...

Embodiment 3

[0061] Weigh 500g of dried grub medicinal material, pulverize it into fine powder; place it in a Soxhlet extractor and extract with 1000mL petroleum ether under reflux for 5 hours, evaporate the residue to dry petroleum ether, dry at 40°C, and then extract with 3000mL 0.2mol / L KOH aqueous solution under reflux 3 times, 2h each time. The combined water extracts were concentrated to 500mL under reduced pressure by a rotary evaporator at 65°C. After cooling to room temperature, 2 times the volume of acetone was added. After standing at 4°C for 6 hours, centrifuge at 3000g for 10 minutes to collect the precipitate. The precipitate was dissolved in 250mL of water, and the Sevag method was adopted (i.e. V 多糖溶液 :V 氯仿 :V 正丁醇 =20︰4︰1 Mix and shake for half an hour, centrifuge, let stand to separate layers, take the water layer) to remove protein, repeat 5 times, centrifuge and discard the precipitate; repeat freezing and thawing the aqueous solution 2 times, centrifuge, discard the p...

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Abstract

The invention belongs to the field of traditional Chinese medicine modernization, and particularly relates to a grub polysaccharide component and a preparation method and application thereof. The grub polysaccharide component has one or more of the following characteristics that monosaccharide is prepared from rhamnose, xylose, glucose and galactose according to the mole ratio of 1:(2.8-3.4):(11.6-12.5):(12.6-13.1), the weight-average molecular weight is 7*10<3>-10*10<3> Da, the total sugar content is 40%-55%, the protein content is 10%-20%, the uronic acid content is 25%-37%, and the sulfuric group content is 8%-11%. The preparation method comprises the steps that after grubs are crushed into coarse powder, total polysaccharide is extracted through alkaline water extraction and alcohol liquid precipitation methods; after deproteinization, depigmentation and dialysis are conducted, grading is conducted with an anion exchange column; the acidic part is separated and purified through size exclusion chromatography, and the obtained component with the molecular weight of 20 kD or below is dialyzed and then concentrated and dried. The grub polysaccharide component has the obvious effect of resisting tumors or reducing blood glucose or reducing blood pressure or resisting oxidation or resisting senescence or enhancing the immunity.

Description

technical field [0001] The invention belongs to the field of modernization of traditional Chinese medicine, and in particular relates to a grub polysaccharide component and a preparation method thereof, and the use of the polysaccharide component in the preparation of anti-tumor, immune-enhancing, blood-sugar-lowering, anti-oxidation, blood-pressure-lowering, anti-aging medicines and health care products application. Background technique [0002] Carbohydrates are one of the three major energy sources (fat, protein, and sugar compounds) for humans or animals and plants. They are an important class of organic compounds widely distributed in nature and play an important role in the life activities of the human body. It is the main source of energy required by all life to sustain life activities. Because sugars are polyhydroxy compounds, and the diversity of hydroxyl stereo configurations, the variability of the bonding positions of polysaccharide monosaccharide residues, the ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P3/10A61P9/12A61P39/06A61P37/04
CPCA61K31/715A61K35/63C08B37/0003C08B37/006
Inventor 曹蔚
Owner HAINAN HONZ PHARMA
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