Separation enrichment and measurement of agricultural product antibiotic residue method
A technology for separation and enrichment and antibiotics, applied in the field of environmental analysis, can solve the problems of time-consuming, low repeatability, and solvent consumption, and achieve the effect of broad prospects, low detection limit, and green separation and enrichment
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Embodiment 1
[0026] (1) Sample pretreatment:
[0027] ①Pork sample: Take a representative pork sample (the fat part should be avoided as much as possible), crush it with a tissue chopper, and homogenize it. Accurately weigh 2 g (accurate to 0.01 g) of a homogeneous sample into a 15 mL centrifuge tube with stopper, add antibiotic internal standard 50 μg / kg, and then add 2 mL phosphate buffer solution (50 mmol / L) and 8 mL Acetonitrile, vortex for 2 min, and centrifuge at 2,500 × g for 10 min at a temperature below 10 °C. Transfer the supernatant to another clean 50 mL centrifuge tube with stopper. Store the filtrate in a refrigerator at 4°C for later use. ② Milk sample: accurately take 5 mL milk sample in a 50 mL centrifuge tube with stopper, weigh and record its quality. Add internal standard antibiotic 4 μg / kg, then add 5 mL phosphate buffer solution (50 mmol / L) and 30 mL acetonitrile, vortex for 2 min, and centrifuge at 2500×g for 10 min at a temperature below 10 ℃. Take 20 mL of the sup...
Embodiment 2
[0030] (1) Sample pretreatment:
[0031] ①Pork sample: Take a representative pork sample (the fat part should be avoided as much as possible), crush it with a tissue chopper, and homogenize it. Accurately weigh 2 g (accurate to 0.01 g) of a homogeneous sample into a 15 mL centrifuge tube with stopper, add antibiotic internal standard 50 μg / kg, and then add 2 mL phosphate buffer solution (50 mmol / L) and 8 mL Acetonitrile, vortex for 2 min, and centrifuge at 2,500 × g for 10 min at a temperature below 10 °C. Transfer the supernatant to another clean 50 mL centrifuge tube with stopper. Store the filtrate in a refrigerator at 4°C for later use. ② Milk sample: accurately take 5 mL milk sample in a 50 mL centrifuge tube with stopper, weigh and record its quality. Add internal standard antibiotic 4 μg / kg, then add 5 mL phosphate buffer solution (50 mmol / L) and 30 mL acetonitrile, vortex for 2 min, and centrifuge at 2500×g for 10 min at a temperature lower than 10 ℃. Take 20 mL of th...
Embodiment 3
[0034] (1) Sample pretreatment:
[0035] ①Pork sample: Take a representative pork sample (the fat part should be avoided as much as possible), crush it with a tissue chopper, and homogenize it. Accurately weigh 2 g (accurate to 0.01 g) of a homogeneous sample into a 15 mL centrifuge tube with stopper, add antibiotic internal standard 50 μg / kg, and then add 2 mL phosphate buffer solution (50 mmol / L) and 8 mL Acetonitrile, vortex for 2 min, and centrifuge at 2,500 × g for 10 min at a temperature below 10 °C. Transfer the supernatant to another clean 50 mL centrifuge tube with stopper. Store the filtrate in a refrigerator at 4°C for later use. ② Milk sample: accurately take 5 mL milk sample in a 50 mL centrifuge tube with stopper, weigh and record its quality. Add internal standard antibiotic 4 μg / kg, then add 5 mL phosphate buffer solution (50 mmol / L) and 30 mL acetonitrile, vortex for 2 min, and centrifuge at 2500×g for 10 min at a temperature lower than 10 ℃. Take 20 mL of th...
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