Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for operating magnetic body particles and device for operating magnetic body particles

A technology of a magnetic substance and an operation method, which is applied in the field of magnetic substance particle manipulation and magnetic substance particle manipulation device fields, can solve the problems of complicated dissolving/fixing operation, increased device manufacturing cost, etc.

Active Publication Date: 2017-02-22
SHIMADZU SEISAKUSHO CO LTD
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there are problems such as complicated dissolving / fixing operations, complicated processing such as installing a partition wall in the device, and increasing the manufacturing cost of the device.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for operating magnetic body particles and device for operating magnetic body particles
  • Method for operating magnetic body particles and device for operating magnetic body particles
  • Method for operating magnetic body particles and device for operating magnetic body particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0102] Hereinafter, the present invention will be described more specifically by using an experimental example of extracting DNA from human whole blood using silica-coated magnetic beads. In addition, this invention is not limited to the following example.

reference example 1

[0104]

[0105] Take 200 μL of human whole blood into a polypropylene tube with a capacity of 1.5 mL (Eppendorf Safe-Lock Tube Cat. No. 0030120.086), add 5 μL of proteinase K (20 mg / mL) aqueous solution, and mix for 10 seconds. Here, 100 μL of dissolving / fixing solution (30 mM Tris-HCl pH 8.0, 30 mM EDTA, 5% Tween-20, 0.5% Triton X-100, 800 mM guanidine hydrochloride) was added and mixed for 10 seconds. Incubate in an aluminum block type constant temperature bath for 5 minutes. Remove the tube from the thermostat, and immediately add magnetic beads with an average particle size of about 3 μm (compared with Toyobo’s nucleic acid extraction kit “MagExtractor”) suspended in isopropanol (75 μL). TM -1 mg of silica-coated magnetic beads for nucleic acid extraction supplied with Genome", and stirred for 5 minutes with a vortex mixer equipped with an adapter for continuous stirring. Set the tube on the rack for magnetic substance particle separation, and after standing for 1 minute...

Embodiment 1

[0111] Take 200 μL of human whole blood into a polypropylene resin tube with a capacity of 1.5 mL, add a dissolving / fixing solution (50 mM Tris-HCl pH 6.4, 10% Triton X-100, 4M guanidine isocyanate) without adding proteinase K, and mix 10 seconds. Here, magnetic beads suspended in isopropanol were added in the same manner as in Reference Example 1 above, and one steel ball (manufactured by Shinto Kogyo Co., Ltd.) with a particle diameter of 1 mm was added. Next, a neodymium magnet (cylindrical shape with a diameter of 6 mm and a length of 23 mm, trade name "NE127" manufactured by Niroku Seisakusho) was moved five times per second along the outer wall surface of the tube at a distance of about 2 cm from the bottom of the tube to the vicinity of the lid. Reciprocating movement at a reciprocating speed. At this time, it was visually confirmed that the magnetic beads were dispersed in the liquid while moving the steel balls following the magnet.

[0112] The tube was set on the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The present invention relates to: a method for operating magnetic body particles that is for fixing a target substance in a liquid sample to the surface of the magnetic body particles; and a device for operating magnetic body particles that is used for said method. The magnetic body particles are particles to which a target substance can be fixed selectively. In this method, in a state where a liquid sample (31), the magnetic body particles (71), and a magnetic solid body (60) that has a larger particle diameter than that of the magnetic body particle are made to coexist in a container (10), the magnetic body particles (71) are moved together with the magnetic solid body (60) within the liquid sample (31) by operating a magnetic field from outside the container. By this operation, the target substance can be fixed selectively to the surface of the magnetic body particles (71).

Description

technical field [0001] The present invention relates to a method of operating magnetic substance particles for selectively immobilizing a target substance in a sample on the surface of the magnetic substance particles. In addition, the present invention relates to an apparatus for handling magnetic substance particles used in the method. Background technique [0002] For medical inspection, food safety and sanitation management, and environmental protection testing, it is required to extract target substances from samples containing various inclusions for detection and reaction. For example, in genetic testing, it is necessary to efficiently extract DNA and RNA from organism-derived samples such as animal and plant blood, serum, cells, urine, feces, and viruses before amplifying target nucleic acid by PCR or the like. [0003] In order to extract and purify the target substance in the sample, the following method has been developed and put into practical use: using a magnet...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): B01J19/08B01J20/28B03C1/00C07K1/22C12N1/00C12N1/02C12Q1/02C12Q1/68G01N1/28G01N33/553
CPCB01J20/28004B01J20/28009B03C1/01B03C1/288B03C1/30B03C2201/18B03C2201/26C07K1/047C07K1/22C12N1/066C12N13/00C12N15/1013G01N33/54326C12Q1/6806G01N1/405G01N2001/4038
Inventor 大桥铁雄
Owner SHIMADZU SEISAKUSHO CO LTD