Membrane spreading oil suspension agent and preparation method and application thereof
A technology of oil suspension and film spreading, which is applied in the direction of botanical equipment and methods, applications, insecticides, etc., can solve the problems of poor spreading performance and fluctuating product control effect, and achieve remarkable control effect, good effect, and spread Cloth performance good effect
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[0021] In the present invention, the preparation method of Paecilomyces fumigatus comprises the following steps:
[0022] 1) activating the Paecilomyces fumigatus species;
[0023] 2) inoculating the activated Paecilomyces fumigatus obtained in step 1) in the primary liquid culture medium of Paecilomyces fumigatus to carry out primary liquid culture to obtain the primary seed liquid of Paecilomyces fumigatus ;
[0024] 3) inoculating the primary seed liquid obtained in step 2) in the secondary liquid culture medium of Paecilomyces fumigatus for secondary liquid culture to obtain the secondary seed liquid of Paecilomyces fumigatus;
[0025] 4) inoculating the secondary seed solution obtained in step 3) into the solid medium of Paecilomyces fumigatus, and performing solid culture to obtain Paecilomyces fumigatus.
[0026] In the present invention, the activation of the Paecilomyces fumigatus strains is carried out by inoculating the preserved Paecilomyces fumigatus strains in ...
Embodiment 1
[0061] Preparation of the primary seed liquid of Paecilomyces fumigatus: Inoculate the preserved Paecilomyces fumigatus strains into eggplant-shaped bottles equipped with nutrient agar medium, and cultivate them at 28°C for 12 hours for activation culture to complete the activation of the strains; The Paecilomyces fumigatus strain after activation is inoculated in the first-level liquid culture medium of Paecilomyces fumigatus according to the inoculation amount of 1%, and the first-level liquid culture medium comprises the component of following mass content: potato flour 33g / L, White sugar 20g / L, peptone 1.0g / L, sodium nitrate 0.1g / L, potassium chloride 0.25g / L, magnesium sulfate 0.05g / L, iron sulfate 0.05g / L, manganese sulfate 0.05g / L, hydrogen phosphate Dipotassium 0.5g / L, the balance is water. The temperature of the primary liquid culture of Paecilomyces fumigatus is 25°C, and the initial pH value is 6.5. 3 / h ventilation for aeration culture, after 24 hours of liquid cu...
Embodiment 2
[0066] In a mixing tank with agitator, Paecilomyces fumosanum 10kg, methyl oleate 5kg, nonylphenol oxyethylene ether 4kg, fatty alcohol polyoxyethylene ether 3kg and white oil 76.6kg prepared in Example 1 were After fully stirring and mixing at 100rpm, sand mill for 200min to particle size D 90 After ≤5μm, add 3 kg of dodecyl alcohol ester, 3 kg of propylene glycol phenyl ether and 5 kg of butyl ether acetate, and stir at 50 rpm to obtain a film-forming oil suspension.
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