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Method for simultaneously preparing six iridoid glycoside components from jasmine

A technology of iridoid glycosides and gardenia, which is applied in the field of traditional Chinese medicine, can solve the problems of extensive extraction process, cumbersome operation process, and environmental pollution, so as to avoid the use of a large amount of toxic organic solvents, simplify the process flow, and reduce pollution Effect

Inactive Publication Date: 2017-03-15
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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Problems solved by technology

Chinese patent (a method for extracting deacetylated cartilinic acid methyl ester and gallinacein methyl ester, CN103242390A) through macroporous resin impurity removal, silica gel column elution and liquid phase separation to obtain deacetylated The two monomer components of acetylpyrroside methyl ester and gallinacein methyl ester, the operation process is cumbersome and pollutes the environment
The Chinese patent (Gardenia iridoids total extract and its preparation method and application, CN1517326A) obtains the total iridoids extract by ethanol percolation, ethanol reflux extraction or water decoction. Obtain monomeric compounds such as geniposide
The above methods can only obtain one or a few iridoid glycoside components, and the operation is cumbersome. At present, there is no method for synchronously preparing 6 kinds of iridoid glycoside components from Gardenia

Method used

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  • Method for simultaneously preparing six iridoid glycoside components from jasmine
  • Method for simultaneously preparing six iridoid glycoside components from jasmine
  • Method for simultaneously preparing six iridoid glycoside components from jasmine

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Embodiment Construction

[0024] 　 The technical solution of the present invention will now be described in detail in conjunction with the embodiments and accompanying drawings. The embodiments are only used to illustrate the present invention, but the scope of protection is not limited thereto.

[0025] 1. Preparation of the alcoholic extract of Gardenia jasminoides: Grind the fruits of Gardenia jasmine into coarse particles, extract 3 times with 10 times the amount of 50% ethanol under reflux, each time for 1.0 h, filter, and combine the filtrates. Recover ethanol under reduced pressure until there is no alcohol smell, and obtain 1 g / mL gardenia alcoholic extract.

[0026] 2. Separation and detection of concentrated sites of iridoid glycosides by macroporous adsorption resin column

[0027] (1) Take gardenia jasminoides alcohol extract extract and disperse and dissolve it with appropriate amount of water, then separate it through HPD-100 macroporous adsorption resin column, use 10 times the colum...

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Abstract

The invention provides a method for simultaneously preparing six iridoid glycoside components from jasmine flowers efficiently and rapidly. The method comprises the steps that jasmine fruit alcohol extracts are subjected to macroporous resin coarse separation, reverse phase intermediate-pressure preparation solution liquid chromatogram enriched flow separation and reverse phase high-pressure separation and purification, and the six monomer compounds with the purity higher than 95% or above can be obtained. The method can enlarge the laboratory separation scale and improve the purifying efficiency; the preparation technology is simple, the cost is low, the extraction efficiency is high, more importantly, samples are not wasted, and the crystallization purity is high.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a separation and purification method for simultaneously preparing six iridoid glycoside components from the traditional Chinese medicine Gardenia jasminoides. 6 monomeric compounds including genizaside, gallinacein methyl ester, genipin-1-β-D-gentiobioside and geniposide. Background technique [0002] Gardenia is a Rubiaceae plant gardenia ( Gardenia jasminoides Ellis) dry and ripe fruit, has the effects of purging fire and eliminating troubles, clearing heat and promoting dampness, cooling blood and detoxification, and reducing swelling and pain for external use. Pharmacological studies have shown that gardenia has antipyretic and sedative effects, protects the liver and promotes gallbladder, and is clinically used to treat acute jaundice hepatitis, pain relief, sprains, coronary heart disease, etc. [0003] The iridoids represented by geniposide are the main medicin...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08A61K36/744
Inventor 张村王云刘慧申立峰姚蓝李玲云
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
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