Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Method for simultaneously preparing six iridoid glycoside components from jasmine

A technology of iridoid glycosides and gardenia, which is applied in the field of traditional Chinese medicine, can solve the problems of extensive extraction process, cumbersome operation process, and environmental pollution, so as to avoid the use of a large amount of toxic organic solvents, simplify the process flow, and reduce pollution Effect

Inactive Publication Date: 2017-03-15
INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
View PDF6 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent (a method for extracting deacetylated cartilinic acid methyl ester and gallinacein methyl ester, CN103242390A) through macroporous resin impurity removal, silica gel column elution and liquid phase separation to obtain deacetylated The two monomer components of acetylpyrroside methyl ester and gallinacein methyl ester, the operation process is cumbersome and pollutes the environment
The Chinese patent (Gardenia iridoids total extract and its preparation method and application, CN1517326A) obtains the total iridoids extract by ethanol percolation, ethanol reflux extraction or water decoction. Obtain monomeric compounds such as geniposide
The above methods can only obtain one or a few iridoid glycoside components, and the operation is cumbersome. At present, there is no method for synchronously preparing 6 kinds of iridoid glycoside components from Gardenia

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for simultaneously preparing six iridoid glycoside components from jasmine
  • Method for simultaneously preparing six iridoid glycoside components from jasmine
  • Method for simultaneously preparing six iridoid glycoside components from jasmine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0024] 　 The technical solution of the present invention will now be described in detail in conjunction with the embodiments and accompanying drawings. The embodiments are only used to illustrate the present invention, but the scope of protection is not limited thereto.

[0025] 1. Preparation of the alcoholic extract of Gardenia jasminoides: Grind the fruits of Gardenia jasmine into coarse particles, extract 3 times with 10 times the amount of 50% ethanol under reflux, each time for 1.0 h, filter, and combine the filtrates. Recover ethanol under reduced pressure until there is no alcohol smell, and obtain 1 g / mL gardenia alcoholic extract.

[0026] 2. Separation and detection of concentrated sites of iridoid glycosides by macroporous adsorption resin column

[0027] (1) Take gardenia jasminoides alcohol extract extract and disperse and dissolve it with appropriate amount of water, then separate it through HPD-100 macroporous adsorption resin column, use 10 times the colum...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for simultaneously preparing six iridoid glycoside components from jasmine flowers efficiently and rapidly. The method comprises the steps that jasmine fruit alcohol extracts are subjected to macroporous resin coarse separation, reverse phase intermediate-pressure preparation solution liquid chromatogram enriched flow separation and reverse phase high-pressure separation and purification, and the six monomer compounds with the purity higher than 95% or above can be obtained. The method can enlarge the laboratory separation scale and improve the purifying efficiency; the preparation technology is simple, the cost is low, the extraction efficiency is high, more importantly, samples are not wasted, and the crystallization purity is high.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a separation and purification method for simultaneously preparing six iridoid glycoside components from the traditional Chinese medicine Gardenia jasminoides. 6 monomeric compounds including genizaside, gallinacein methyl ester, genipin-1-β-D-gentiobioside and geniposide. Background technique [0002] Gardenia is a Rubiaceae plant gardenia ( Gardenia jasminoides Ellis) dry and ripe fruit, has the effects of purging fire and eliminating troubles, clearing heat and promoting dampness, cooling blood and detoxification, and reducing swelling and pain for external use. Pharmacological studies have shown that gardenia has antipyretic and sedative effects, protects the liver and promotes gallbladder, and is clinically used to treat acute jaundice hepatitis, pain relief, sprains, coronary heart disease, etc. [0003] The iridoids represented by geniposide are the main medicin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H17/04C07H1/08A61K36/744
Inventor 张村王云刘慧申立峰姚蓝李玲云
Owner INST OF CHINESE MATERIA MEDICA CHINA ACAD OF CHINESE MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com