A kind of method for the production of liprestatin by multi-stage fermentation

Abstract

The invention discloses a method for producing Lipstatin through multi-stage fermentation, comprising the stages of strain activation, seed culture and fermentation culture. The first 10-40 hours of the fermentation culture stage is a cell growth stage, with dissolved oxygen level controlled at 50-100%. The Lipstatin production stage occurs after 40 hours. When the wet weight of bacteria reaches 10-12 g / L, the bacteria are transferred into a micro-oxygen fermentation stage, with the dissolved oxygen level controlled at 20-30%. When glucose concentration is less than 1 g / L, flow feeding with a substrate I is started, and fermentation is conducted for 60-80 hours with the glucose concentration in a fermentation tank controlled in the range of 1.0-1.5 g / L. 10-20 hours after feeding with the substrate I, flow feeding with a substrate II is begun, and flow feeding with a substrate III is begun 20-30 hours after feeding with the substrate I. The fermentation adopts multi-stage dissolved oxygen control and feeding fermentation in batches, and makes more than 80% of the product secreted outside the mycelium by multi-stage fermentation production of Lipstatin and simultaneously controls the permeability of Streptomyces mycelium. The method has the advantages of low cost, simple operation, short fermentation time, high yield of Lipstatin and easy separation of the product, and is beneficial to industrial production.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a method for producing riprestatin by multi-stage fermentation. Background technique [0002] With the development of the economy, great changes have taken place in the diet life. People consume more junk food and sugar, and lack of exercise, which leads to excessive accumulation and abnormal distribution of fat, causing obesity. Obesity can cause disturbances in the levels of many parameters in the body, such as blood sugar, insulin, and blood lipids. Of course, in recent years, scientists have paid more and more attention to the relationship between obesity and cancer. [0003] The pancreatic lipase inhibitor liprestatin is produced by the metabolism of Streptomyces toxins. It selectively inhibits pancreatic lipase in the gastrointestinal tract to reduce the decomposition and absorption of fat by 30%, so as to achieve the purpose of weight loss. It ...

AI Technical Summary

Problems solved by technology

[0005] At present, domestic research on Lipstatin fermentation is mostly focused on the optimization of culture medium and culture conditions, but there are still problems such as long fermentation cycle, complicated extraction and separation steps and low yield.

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