A kind of biological control method that stimulates Cryptocaryoniasis
A technology that stimulates Cryptocaryoniasis and stimulates Cryptocaryoniasis can be applied in applications, climate change adaptation, fish farming, etc. It can solve the problems of time-consuming, labor-intensive and expensive dragging of cages, and difficulty in large-scale application, so as to strengthen prevention and control Effects, highly operable effects
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Embodiment 1
[0027] Biological control of Cryptocaryon irritans with clam
[0028] 1 Basis for the selection of clams
[0029] Put 5 clams in 5 20L water tanks, and their wet weights are 32.5g, 36.2g, 41.1g, 33.6g and 35.6g respectively. The other 5 20L tanks do not contain clams. Count the ciliated larvae within 2 hours of incubation and place them in 10 tanks at a density of 100 / mL. After placing ciliated larvae for 3h, 6h, 12h and 24h, take 2mL water samples in the 4 corners and the middle water layer of the center of each tank respectively with a pipette and put them in a 50mL small beaker and fix with 10% formaldehyde for 10min. Allow it to settle at the bottom of a small beaker. Then, carefully suck up the upper layer of sea water, and transfer all the remaining about 5 mL of sea water with ciliated larvae to the observation dish. Shake gently to concentrate the ciliated larvae in the center of the observation dish and count under the microscope. Repeat sampling and count 3 times, an...
Embodiment 2
[0035] According to Table 1, the filtration rate of the soft clam dry weight 1.38g is 3.0L / (g﹒h). The dry weight of the shellfish soft part is about 25 times the wet weight of the shell, so the wet weight of 1.38g of clam is about 35g. Therefore, the filtration rate of clams calculated by fresh weight is 0.12L / (g﹒h).
[0036] According to Q t = 2V s / 1000CR can calculate 40m 3 The amount of clams used in indoor cement pool treatment = 2×40×10 3 ÷0.12L / (g﹒h)÷1000=666kg. Every 20kg is evenly installed in a cage, and a total of 33 cages are evenly suspended in the cement.
[0037] 40m 3 Indoor cement ponds breed 1,000 groupers of about 100 g. It was found that the fish did not eat enthusiastically and their activity decreased. Take 10 groupers randomly. The mucus was scraped from the surface of each fish, and it was found that there were 1-2 Cryptocaryon irritans under a microscope field of 400 times. Confirm that the infection stimulates Cryptocaryon irritans. Hang the clams ev...
Embodiment 3
[0039] According to Table 1, the filtration rate of the hat oyster with the dry weight of the soft part of 0.98g is 74.8mL / (g﹒min). The dry weight of the soft part is 0.98g equivalent to 25g of fresh weight. Therefore, the filtration rate calculated by wet weight is 2.9mL / (g﹒min), which is 0.17L / (g﹒h).
[0040] According to Q t = 2V s / 1000CR can calculate the amount of hat oysters used in shallow sea culture cage treatment of 4×4×3=2×4×4×3×10 3 ÷0.17L / (g﹒h)÷1000=565kg. Every 20kg is evenly installed in a cage, and a total of 28 cages are evenly suspended in the cage.
[0041] A 4×4×3 shallow sea aquaculture cage is used to cultivate 500 groupers of about 200 g in size. Fish eat and move normally. When patrolling at night, when the flashlight illuminates the fish body, some fish bodies are occasionally found with white spots. Take 10 fish to scrape the mucus for microscopic examination. There are 5-10 Cryptocaryon irritans in the mucus of each fish in a 400-fold field of view....
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