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Preparation method for crop fungicide

A technology for fungicides and crops, applied in the field of fungicides preparation, can solve the problems of poor broad-spectrum fungicides, environmental pollution, and enhanced resistance of pathogenic bacteria, and achieve the effects of significant bactericidal effect, effective prevention and control methods, and convenient use.

Inactive Publication Date: 2017-03-22
CHANGZHOU YAHUAN ENVIRONMENTAL PROTECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem mainly solved by the present invention: for the existing crop fungicides have poor broad-spectrum and poor control effect, long-term application will easily lead to enhanced resistance of pathogenic bacteria, doubling of the application amount, and easy to cause serious serious diseases during use. In order to solve the problem of environmental pollution, a preparation method of a crop fungicide is provided. In the method, at first Bacillus polymyxa is inoculated into the culture medium, and the resulting culture is centrifuged, and the bacteria in the lower layer are collected together with mashed potatoes, sucrose, and soybean cake. Powder and other mixed fermentation, after high temperature sterilization, collect the fermented liquid and centrifuge to collect the supernatant, then crush the cloves, cinnamon and aloe, mix with rice wine and ultrasonically extract, and finally mix the obtained extract with the supernatant and fish essence Protein, tung oil, etc. are stirred and packaged to obtain crop fungicides. The antibacterial substances produced by the fermentation of Bacillus polymyxa can kill and control pathogenic bacteria through site competition and induction of resistance. Protamine has a strong Bactericidal ability and broad-spectrum antibacterial property, the crop fungicide prepared by the present invention is easy to use, can adopt various methods such as soaking seeds, root irrigation, spraying treatment, etc., and the fungicide has remarkable bactericidal and control effects, effectively reducing the use of fungicides The occurrence and development of quantity and resistance, and can expand the bactericidal spectrum. While reducing the cost of medication, it also reduces the impact on the environment and has a broad application space.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] First, weigh 10g of glucose, 5g of peptone, 3g of beef extract, 0.5g of sodium chloride and 800mL of deionized water in turn, add them to a petri dish, stir and mix evenly, transfer to a sterilizing tank, and sterilize at 100°C After 5 minutes, the medium was prepared, and then Bacillus polymyxa was inoculated into the medium according to the inoculation amount of 8%, and the medium was placed in a shaker, and cultivated at 35°C and 120r / min for 12h; Place the culture in a centrifuge, centrifuge at 1000r / min for 30min, collect the bacteria in the lower layer, and weigh 30g of mashed potatoes, 10g of sucrose, 3g of soybean cake powder, 1g of disodium hydrogen phosphate, 0.3g of magnesium sulfate and 800mL of deionized Water, stirred and mixed, placed in a fermenter, 6g of the above-mentioned lower-layer bacteria was added to the fermenter, fermented at 25°C for 3 days, after the fermentation was completed, the temperature was raised to 100°C for high-temperature steriliza...

example 2

[0019]First, weigh 15g of glucose, 7g of peptone, 4g of beef extract, 0.7g of sodium chloride and 900mL of deionized water in sequence, add them to a petri dish, stir and mix evenly, transfer to a sterilizing tank, and sterilize at 105°C 8min, prepare the medium, then inoculate Bacillus polymyxa into the medium according to the inoculum amount of 10%, put the medium in a shaker, and cultivate it at 40°C and 130r / min for 18h; Place the culture in a centrifuge, centrifuge at 1100r / min for 35min, collect the bacteria in the lower layer, then weigh 35g of mashed potatoes, 13g of sucrose, 4g of soybean cake powder, 2g of disodium hydrogen phosphate, 0.4g of magnesium sulfate and 900mL of deionized Water, stirred and mixed, placed in a fermenter, 7g of the above-mentioned lower-layer bacteria was added to the fermenter, fermented at 30°C for 3.5 days, after the fermentation was completed, the temperature was raised to 105°C for high-temperature sterilization for 8min, and then the fe...

example 3

[0022] First, weigh 20g of glucose, 8g of peptone, 5g of beef extract, 0.8g of sodium chloride and 1000mL of deionized water in turn, add them to a petri dish, stir and mix evenly, transfer to a sterilizing tank, and sterilize at 110°C After 10 minutes, the medium was prepared, and then Bacillus polymyxa was inoculated into the medium according to the inoculation amount of 12%, and the medium was placed in a shaker, and cultivated at 45°C and 140r / min for 24h; Place the culture in a centrifuge, centrifuge at 1200r / min for 40min, collect the bacteria in the lower layer, and weigh 40g of mashed potatoes, 15g of sucrose, 5g of soybean cake powder, 3g of disodium hydrogen phosphate, 0.5g of magnesium sulfate and 1000mL of deionized Water, stirred and mixed, placed in a fermenter, 8g of the above-mentioned lower-layer bacteria was added to the fermenter, fermented at 35°C for 4 days, after the fermentation was completed, the temperature was raised to 110°C for high-temperature steri...

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PUM

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Abstract

The invention discloses a preparation method for a crop fungicide, and belongs to the technical field of fungicide preparation. The preparation method comprises the following steps of firstly, inoculating a culture medium with bacillus polymyxa and performing culture; then, centrifugally treating obtained culture, collecting strains at a lower layer to be mixed with mashed potatoes, saccharose, soybean cake meal and the like, performing fermentation, performing sterilizing at the high temperature, collecting fermentation liquor, centrifugally treating the fermentation liquor, collecting the obtained supernatant, crushing clove, cinnamon and aloes, mixing the crushed clove, cinnamon and aloes with yellow rice or millet wine, and performing ultrasonic extraction treatment on the mixture; and finally, stirring the obtained extract, the supernatant, protamine, tung oil and the like, and performing packaging, thereby obtaining the crop fungicide. The crop fungicide prepared by the preparation method is convenient to use, and can adopt various ways such as seed soaking, root irrigating and spraying; moreover, the fungicide is remarkable in sterilization and control effect, effectively reduces usage amount of the fungicide as well as occurrence and development of resistance, can expand a bacteriocidal spectrum, reduces influences on environment while drug cost is reduced, and has a wide application space.

Description

technical field [0001] The invention discloses a method for preparing a fungicide for crops, and belongs to the technical field of fungicide preparation. Background technique [0002] There are many types of crops in my country, with a wide distribution range, and relatively many types of diseases. According to the survey, there are more than 2,000 kinds of various diseases of crops in the country, and more than 700 kinds of serious ones occur. The losses caused by crop diseases every year are huge, seriously affecting the development of agriculture. Although people have developed a variety of medicaments for disease prevention and control, and achieved good control effects, most chemical pesticides will bring relatively large negative effects, especially the harm of pesticides to humans and animals and the damage to the natural environment. Pollution and other issues pose a greater threat to human health and the ecological environment. A series of problems such as pesticid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/42A01N63/02A01P1/00A01P3/00
CPCA01N63/10A01N65/00A01N65/42A01N2300/00
Inventor 高玉梅孟中立薛蕾
Owner CHANGZHOU YAHUAN ENVIRONMENTAL PROTECTION TECH
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