Preparation method for crop fungicide
A technology for fungicides and crops, applied in the field of fungicides preparation, can solve the problems of poor broad-spectrum fungicides, environmental pollution, and enhanced resistance of pathogenic bacteria, and achieve the effects of significant bactericidal effect, effective prevention and control methods, and convenient use.
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example 1
[0016] First, weigh 10g of glucose, 5g of peptone, 3g of beef extract, 0.5g of sodium chloride and 800mL of deionized water in turn, add them to a petri dish, stir and mix evenly, transfer to a sterilizing tank, and sterilize at 100°C After 5 minutes, the medium was prepared, and then Bacillus polymyxa was inoculated into the medium according to the inoculation amount of 8%, and the medium was placed in a shaker, and cultivated at 35°C and 120r / min for 12h; Place the culture in a centrifuge, centrifuge at 1000r / min for 30min, collect the bacteria in the lower layer, and weigh 30g of mashed potatoes, 10g of sucrose, 3g of soybean cake powder, 1g of disodium hydrogen phosphate, 0.3g of magnesium sulfate and 800mL of deionized Water, stirred and mixed, placed in a fermenter, 6g of the above-mentioned lower-layer bacteria was added to the fermenter, fermented at 25°C for 3 days, after the fermentation was completed, the temperature was raised to 100°C for high-temperature steriliza...
example 2
[0019]First, weigh 15g of glucose, 7g of peptone, 4g of beef extract, 0.7g of sodium chloride and 900mL of deionized water in sequence, add them to a petri dish, stir and mix evenly, transfer to a sterilizing tank, and sterilize at 105°C 8min, prepare the medium, then inoculate Bacillus polymyxa into the medium according to the inoculum amount of 10%, put the medium in a shaker, and cultivate it at 40°C and 130r / min for 18h; Place the culture in a centrifuge, centrifuge at 1100r / min for 35min, collect the bacteria in the lower layer, then weigh 35g of mashed potatoes, 13g of sucrose, 4g of soybean cake powder, 2g of disodium hydrogen phosphate, 0.4g of magnesium sulfate and 900mL of deionized Water, stirred and mixed, placed in a fermenter, 7g of the above-mentioned lower-layer bacteria was added to the fermenter, fermented at 30°C for 3.5 days, after the fermentation was completed, the temperature was raised to 105°C for high-temperature sterilization for 8min, and then the fe...
example 3
[0022] First, weigh 20g of glucose, 8g of peptone, 5g of beef extract, 0.8g of sodium chloride and 1000mL of deionized water in turn, add them to a petri dish, stir and mix evenly, transfer to a sterilizing tank, and sterilize at 110°C After 10 minutes, the medium was prepared, and then Bacillus polymyxa was inoculated into the medium according to the inoculation amount of 12%, and the medium was placed in a shaker, and cultivated at 45°C and 140r / min for 24h; Place the culture in a centrifuge, centrifuge at 1200r / min for 40min, collect the bacteria in the lower layer, and weigh 40g of mashed potatoes, 15g of sucrose, 5g of soybean cake powder, 3g of disodium hydrogen phosphate, 0.5g of magnesium sulfate and 1000mL of deionized Water, stirred and mixed, placed in a fermenter, 8g of the above-mentioned lower-layer bacteria was added to the fermenter, fermented at 35°C for 4 days, after the fermentation was completed, the temperature was raised to 110°C for high-temperature steri...
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